EPR Plasmid #560: CVS-N2c-nl.mCherry-GCaMP7s
Sequence: download sequence
| Purpose of plasmid | Generation of RVdG-CVS-N2c particles for Trans-synaptic retrograde labeling |
| Depositing Lab |
Peter Jonas Institute of Science and Technology Austria (ISTA) |
| Depositing Scientist | Flavia Leite |
| Antibiotic Resistance | Ampicillin |
| Bacterial Growth Temp | 37 |
| Copy Number | Low |
| Selectable Marker | |
| Retransformation Info: | Retransforms well into TOP10 bacteria, but not into XL1blue. |
| Reference |
Fast, high-throughput production of improved rabies viral vectors for specific, efficient and versatile transsynaptic retrograde labeling. Sumser A et al. Elife 2022 PubMed DOI |
| MTA and Terms |
MTA (PDF download) Ancillary Terms for UCSD Tsien FP Material |
How to cite this reagent:
In the Materials & Methods:
CVS-N2c-nl.mCherry-GCaMP7s was a gift from Peter Jonas (European Plasmid Repository plasmid #560).
In the References:
Sumser A et al.. Fast, high-throughput production of improved rabies viral vectors for specific, efficient and versatile transsynaptic retrograde labeling.. Elife 2022 doi:10.7554/eLife.79848 PubMed 36040301
CVS-N2c-nl.mCherry-GCaMP7s was a gift from Peter Jonas (European Plasmid Repository plasmid #560).
In the References:
Sumser A et al.. Fast, high-throughput production of improved rabies viral vectors for specific, efficient and versatile transsynaptic retrograde labeling.. Elife 2022 doi:10.7554/eLife.79848 PubMed 36040301
Sequence: download sequence
ACGCTTAACAACAAAACCAGAGAAGAAAAAGACAGCGTCAATTGCAAAGCAAAAATGTAACACCCCTACAATGGATGCCGACAAGATTGTGTTCAAAGTCAATAATCAGGTGGTCTCTTTGAAGCCTGAGATTATCGTGGATCAATATGAGTACAAGTACCCTGCCATCAAGGATTTGAAAAAGCCTTGTATCACCCTAGGGAAAGCCCCCGACTTGAACAAAGCATACAAATCAGTTTTATCAGGCATGAATGCCGCCAAACTTGATCCGGATGATGTATGCTCCTACTTGGCAGCAGCAATGCAGTTCTTTGAGGGGACATGTCCGGAAGACTGGACCAGCTATGGAATCCTGATTGCACGAAAAGGAGATAGGATCACCCCAAACTCTCTAGTGGAGATAAAGCGTACTGATGTAGAAGGGAATTGGGCTCTGACAGGAGGCATGGAATTGACAAGGGACCCCACTGTCTCTGAACATGCATCTTTAGTCGGTCTTCTCCTGAGTCTGTACAGGTTGAGCAAAATATCAGGACAGAACACTGGTAACTATAAGACAAACATTGCAGATAGGATAGAGCAGATTTTCGAGACAGCACCTTTTGTTAAGATCGTGGAACACCATACCCTAATGACAACTCACAAGATGTGTGCTAATTGGAGTACTATACCGAACTTCAGATTTTTGGCCGGAACCTACGACATGTTTTTCTCACGGATTGAGCATCTATATTCGGCAATCAGAGTGGGCACAGTCGTCACCGCTTATGAAGACTGCTCAGGACTGGTATCGTTTACAGGGTTCATAAAGCAGATCAATCTCACCGCAAGGGAAGCAATACTATATTTCTTCCACAAGAACTTTGAGGAAGAGATAAGAAGAATGTTCGAGCCAGGGCAAGAGACAGCTGTTCCTCACTCTTATTTCATCCACTTCCGTTCACTAGGCTTGAGTGGGAAGTCTCCTTATTCATCGAATGCTGTCGGTCATGTGTTCAATCTCATTCACTTTGTTGGATGCTACATGGGTCAAGTCAGATCTCTAAATGCGACGGTTATTGCTGCGTGTGCCCCTCATGAGATGTCTGTTCTAGGGGGCTATTTGGGAGAGGAATTCTTCGGAAAAGGGACATTTGAAAGAAGGTTCTTCAGAGACGAGAAAGAACTTCAAGAATATGAGGCGGCTGAACTAACAAAGTCCGACGTGGCACTGGCAGATGACGGAACCGTCAACTCTGATGACGAGGACTATTTCTCTGGTGAAACCAGAAGTCCAGAAGCTGTCTATACTCGAATCATGATGAATGGAGGTCGACTGAAGAGATCTCATATACGGAGATATGTCTCAGTCAGTTCCAATCATCAAGCCCGTCCAAACTCATTCGCCGAATTTTTAAACAAGACGTATTCGAATGACTCATAAGGAGTTGATTGACAGGGTGCCAGAAATCTATAGATTGTATATATCCATCATGAAAAAAACTAACACTCCTCCTTTCAAACCATCCCAAATATGAGCAAGATCTTTGTTAATCCGAGTGCAATCAGAGCCGGTCTGGCCGATCTTGAGATGGCCGAAGAGACTGTTGATCTGATCAACAGAAACATAGAAGACAATCAGGCTCATCTCCAGGGAGAACCCATAGAAGTGGACAACCTCCCTGAGGACATGAAGCGACTTCACCTGGACGATGAAAAATCGTCCAACCTTGGTGAGATGGTTAGGGTGGGAGAAGGCAAGTATCGAGAGGACTTTCAGATGGATGAGGGAGAGGACCCCAACCTCCTGTTCCAATCGTACCTGGATAATGTTGGAGTCCAAATAGTCAGACAAATGAGGTCAGGAGAGAGATTCCTCAAGATATGGTCACAGACCGTAGAGGAAATTGTATCCTATGTCACGGTCAACTTTCCTAACCCTCCAAGAAGGTCTTCGGAGGATAAATCAACCCAGACTACTGGCAGAGAGCTCAAGAAGGAGACAACGTCTGCTTTCTCTCAGAGAGAAAGCCAACCTTCGAAAGCTAGGATGGTGGCTCAAGTTGCCCCTGGTCCTCCAGCCCTTGAATGGTCAGCCACCAATGAAGAAGATGATCTATCAGTAGAGGCTGAGATCGCTCATCAGATTGCTGAAAGCTTTTCCAAGAAGTACAAGTTTCCCTCCCGATCTTCAGGAATATTCTTGTATAATTTTGAGCAACTGAAGATGAACCTTGATGACATAGTTAAAGAGGCAAAAAATGTACCGGGCGTGACCCGTCTGGCCCATGATGGATCCAAAATCCCCCTGAGATGCGTACTGGGATGGGTCGCTTTGGCTAATTCCAAAAAATTCCAATTACTAGTCGAGGCTGACAAGCTAAGCAAAATCATGCAAGATGATTTGAATCGCTATACATCCTGCTAACCGAGTTTTCGAACTCAGTCCCTCCAGATAATGAAAACTGAGATGTTATGGAGTAGACATGAAAAAAACAGGCAACACCACTGATAAAATGAACGTTCTACGCAAGATAGTGAAAAAATGTAGGGATGAGGACACTCAAAAGCCCTCTCCTGTGTCAGCCCCTCCGTATGACGATGACCTGTGGCTTCCACCTCCTGAATATGTCCCGCTGAAAGAACTCACAAGCAAGAAGAACATGAGGAACTTTTGTGTCAACGGGGAGGTTAAAGCGTGTAGCCCAAATGGTTACTCATTCAGGATTTTGCGGCACATTCTGAGATCATTCAACGAGATATACTCTGGGAATCATAGGATGATTGGGTTAGTCAAAATTGTTGTTGGACTAGCTTTATCAGGAGCTCCAGTACCTGAGGGCATGAACTGGGTATACAAATTGAGGAGAACCCTTATATTCCAGTGGGCTGATTCCAGGGGCCCTCTTGAAGGGGAGGAGTTAGATTACTCTCAAGAGATCACTTGGGATGATGATACTGAATTCGTCGGATTGCAAATAAGAGTGAGCGCAAGACAATGTCATATTCAAGGCAGGATCTGGTGTATCAACACGAACTCGAGGGCATGTCAACTATGGTCTGACATGTCTCTTCAGACACAAAGGTCTGAAGAGGACAAAGACTCTTCTCTGCTTCTAGAATAATCAGATTATATCCTGCAAGTGTATCACTTGTTTACCTCTGGAGGAGAGAGCATACAGGCTTGACTCCGATCCTTGGGAGCAATAGAACAAAAAAACACACGTTATGGTGCCGTTAAATCGCTGCATTTTATCAAAGTCAAGTTGATAACCTTTACATTTTGAGCCTCTTGGATGTGAAAAAAACTATTAACATCCCTCAAAAGACCCCGGtaaagccaccatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccctgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagagatctcgagctgatccaaaaaagaagagaaaggtagatccaaaaaagaagagaaaggtagatccaaaaaagaagagaaaggtaggatccagataactgatcataatcagccataccacatttggaattcgtcgagggggcgcgccGCTAGACATGAAAAAAActAACACTCCTCCggtacgccaccatgggttctcatcatcatcatcatcatggtatggctagcatgactggtggacagcaaatgggtcgggatctgtacgacgatgacgataaggatctcgccaccatggtcgactcatcacgtcgtaagtggaataagacaggtcacgcagtcagagctataggtcggctgagctcactcgagaacgtctatatcaaggccgacaagcagaagaacggcatcaaggcgaacttcaagatccgccacaacatcgaggacggcggcgtgcagctcgcctaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcgtgcagtccaaactttcgaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagggcggtaccggagggagcatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggtgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacatccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagcttaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaacctgccggaccaactgactgaagagcagatcgcagaatttaaagagctgttctccctatttgacaaggacggggatgggacaataacaaccaaggagctggggacggtgatgcggtctctggggcagaaccccacagaagcagagctgcaggacatgatcaatgaagtagatgccgacggtgacggcacaatcgacttccctgagttcctgacaatgatggcaagaaaaatgaaatacacagacagtgaagaagaaattagagaagcgttccgtgtgtttgataaggatggcaatggctacatcagtgcagcagagcttcgccacgtgatgacaaaccttggagagaagttaacagatgaagaggttgatgaaatgatcagggaagcagacatcgatggggatggtcaggtaaactacgaagagtttgtacaaatgatgacagcgaagtgaCGGCGCCTAGCCGGTCATCCTTTTGACGATTCCAGTCCCGAGGATAACCTCCTCTCGGGATTGGGGGGAATCTTTGGATCCAGTAGTCCTCCTTGAACTCCGTCCAACAGGGCAGATTCAAGAGTCATAAGACTTTCATTAATCATTTCAGTTGATCAGACATGGTCGTGTAGATTCTCTTAATACGGGAGATCTTCTAGCAGTTTCAGTGACCAACGGTGCTTTCATTCTCCAGGAACTGATACCAAAGGTTGTGGACAGGCCAAGGGGTACTTCGGATGACTCTGTGCTTGGGCACAGAAAGAGGTCGTAGTGTGCCCCCCGATAGCGGACTCGACATGAATCAACTAAGAAAGGCAATCTGCCTCCCATGATGGACATAAGCAATAGTTCACAACCATCTTGCATCTCAGTGAAGTGTACATAACTATTGAGGGCTGGGTCATCTAAGCATTTCAGTCGAGAAAAAAACTGTAGACCAAAAGAACAACTAGCAACACTTCTCATCCAGAGACCCATATCAAGATGCTAGATCCGGGAGAGGTTTATGATGACCCTATTGATCCAATTGAGTCAGAGGCTGAACCCAGAGGAACCCCCACTGTCCCCAACATCTTGAGGAACTCCGACTACAATCTCAATTCTCCTTTGATAGAGGATCCTGCCAAACTAATGTTAGAATGGTTGAAGACAGGGAACAGACCTTATCGGATGACTTTGACAGACAATTGCTCCAGGTCTTACAAAGTTTTGAAAGATTATTTCAAGAAAGTAGATTTGGGTTCTCTCAAAGTGGGCGGAACTGCTGCACAGTCAATGGTTTCTCTCTGGTTGTGTGGTGCCCACTCTGAATCAAACAGGAGCCGGAGATGTATAACCGACTTGGCCCATTTCTATTCCAAGTCATCCCCCATAGAGAAGCTATTGAATTGTACGCTAGGAAACAGAGGCCTGAGAATCCCACCAGAGGGGGTGTTAAATTGCCTCGAGAGGGTCAATTATGACAAGGCATTTGGGAGGTATCTGGCCAACACGTATTCCTCTTACTTGTTTTTCCATGTAATCACCTTATACATGAATGCCTTAGACTGGGAAGAGGAAAAGACCATCCTGGCATTATGGAAAGATATAACCTCAGTGGATACCGAGAAGGACTTGGTCAAATTCAAAGATCAAATATGGGGACTGTTGATTGTGACAAAGGACTTTGTTTACTCTCAGAGTTCAAACTGTCTTTTTGACAGAAACTACACACTGATGCTAAAGGATCTTTTCTTGTCTCGATTCAACTCCTTAATGATTCTGCTTTCTCCCCCTGAGCCCCGATACTCAGATGACTTAATATCTCAGCTGTGCCAGCTATACATCGCTGGGGATCAAGTCTTGTCCATGTGTGGGAACTCCGGCTATGAAGTCATCAAAATATTGGAGCCATATGTCGTGAACAGTTTGGTCCAGAGGGCAGAGAAGTTTAGGCCTCTCATCCACCCCTTGGGAGACTTTCCTATGTTCATAAAAGACAAGGTGAATCAACTTGAAGGGACTTTTGGTCCCAGTGCAAAGAGGTTTTTTAGGGTTTTAGATCAATTCGACAACATACATGACTTAGTATTTGTGTATGGCTGTTACAGACATTGGGGGCACCCCTATATAGATTATCGGAAGGGTCTGTCGAAACTATATGATCAAGTTCACATTAAGAAAGTAATAGATAAGTCCTACCAGGAGTGTTTAGCAAGTGACTTGGCCAGAAGGATCCTCAGATGGGGATTTGACAAGTACTCCAAATGGTATCTAGATTCGAGATTCCTTGCCCGAGACCACCCCTTGACTCCTTATGTCAAGACCCAAACATGGCCACCCAAACATATAGTAGACTTGGTGGGGGACACATGGCATAAGCTCCCGATCACGCAGATCTTTGAAATTCCTGAATCAATGGACCCGTCAGAGATACTGGATGACAAATCACATTCTTTCACCAGAACAAGGTTAGCTTCTTGGCTGTCAGAGAACCGAGGGGGGCCTGTTCCTAGCGAGAAGGTCATTATCACGGCCCTGTCTAAGCCACCTGTCAATCCCCGAGAGTTTTTGAAATCTATAGACCTCGGAGGATTGCCAGATGAGGATTTGATAATTGGCCTCAAACCAAAGGAACGGGAGTTGAAGATCGAGGGCCGATTCTTTGCTCTAATGTCATGGAATCTAAGATTATATTTTGTCATCACCGAAAAGCTCTTGGCCAACTACATTTTGCCACTTTTTGACGCACTGACTATGACAGACAACCTGAACAAGGTGTTCAAAAAGTTGATCGACAGGGTCACCGGGCAAGGGCTTTTGGACTATTCTAGGGTCACATACGCATTTCACCTGGACTATGAGAAATGGAACAATCATCAAAGATTGGAGTCAACAGAGGATGTATTCTCTGTCCTAGATCAGGTGTTTGGATTGAAGAGGGTGTTTTCTAGAACACACGAGTTTTTTCAGAAGTCCTGGATCTATTATTCAGACAGATCAGACCTCATTGGGTTATGGGAGGACCAAATATATTGCTTGGATATGTCTAACGGCCCAACCTGCTGGAATGGCCAAGATGGCGGGCTAGAGGGCTTGCGGCAGAAGGGCTGGAGTCTAGTCAGTTTATTAATGATAGATAGAGAATCTCAAACCAGGAACACAAGAACCAAGATACTAGCTCAAGGAGACAACCAGGTTCTGTGTCCTACATACATGTTGTCACCGGGATTGTCTCAAGAGGGGCTTCTCTATGAGTTAGAGAGCATATCAAGGAATGCACTCTCAATATACCGAGCTATCGAGGAAGGAGCATCTAAGCTGGGGCTGATCATCAAGAAGGAAGAGACCATGTGTAGTTATGACTTTCTCATATATGGGAAGACCCCCTTATTTCGAGGCAACATATTGGTGCCTGAATCCAAAAGATGGGCCCGAGTCTCTTGCATCTCTAACGACCAAATAGTCAACCTCGCCAATATAATGTCGACAGTATCCACCAATGCGCTGACAGTGGCACAACACTCTCAATCTCTGATCAAACCTATGAGGGATTTTCTGCTCATGTCAGTACAGGCAGTTTTCCACTACCTGTTGTTTAGCCCAATCCTAAAAGGCAGAGTTTATAAGATTCTGAGTGCTGAAGGGGAGAGCTTTCTCCTAGCCATGTCGCGGATAATCTACCTAGATCCTTCTTTGGGAGGGGTGTCTGGAATGTCTCTCGGGAGGTTCCATATACGTCAGTTCTCAGACCCTGTCTCTGAAGGGTTGTCATTCTGGAGAGAGATCTGGTTAGGCTCTCATGAGTCCTGGATTCACGCGTTGTGTCAGGAGGCCGGGAACCCCGATCTTGGAGAGAGAACACTCGAGAGCTTCACTCGCCTTTTAGAAGATCCTACTACCTTAAATATCAAAGGAGGGGCCAGCCCTACCATTCTACTCAAGGATGCTATCAGAAAGGCTCTGTACGACGAGGTGGACAAGGTGGAAAATTCAGAGTTTCGAGAGGCAATCCTGCTGTCCAAGACCCATAGAGATAACTTTATACTCTTTTTAAAATCTGTTGAGCCTCTGTTCCCTCGATTTCTCAGTGAGCTCTTCAGTTCGTCTTTCTTGGGAATACCGGAGTCAATCATTGGACTGATACAAAACTCCCGGACAATAAGAAGGCAGTTTAGAAAGAGTCTCTCAAGAACTTTAGAAGAGTCCTTCTACAACTCAGAGATCCACGGGATTAATCGGATAACCCAGACACCTCAAAGGGTCGGAAGGGTGTGGCCTTGCTCTTCAGAGAGGGCGGATCTACTTAGGGAGATCTCTTGGGGGAGGAAAGTGGTAGGCACGACAGTTCCTCACCCTTCCGAGATGTTGGAGTTGTTTCCCAAATCCTCCATTTCCTGCACTTGTGGAGCAACAGGGGGAGGCAATCCTAGAGTCTCTGTATCAGTACTCCCGTCCTTCGATCAGTCATTTTTCTCACGGGGCCCCCTAAAGGGATACTTGGGCTCGTCCACCTCTATGTCAACCCAGCTATTCCATGCATGGGAAAAAGTCACTAATGTTCATGTGGTGAAAAGGGCTCTATCGTTAAAAGAATCTATAAACTGGTTCATCAATAGGAATTCCAATTTGGCTCAAACTCTAATTGGAAACATCATGTCTCTGACAGGCCCTGACTTCCCTCTAGAAGAGGCCCCTGTTTTCAAACGGACAGGGTCAGCCTTGCATAGGTTCAAGTCTGCCAGATACAGCGAAGGAGGGTATTCTTCTGTTTGCCCTAACCTTCTCTCCCATATCTCTGTTAGTACAGACACTATGTCTGATTTGACCCAAAACGGGAAGAACTATGATTTCATGTTTCAGCCATTGATGCTTTATGCGCAAACATGGACATCGGAACTGGTACAGAGGGATACAAGACTTAGAGACTCCACGTTTCACTGGCACCTTCGGTGCAACAGATGTGTGAGGCCCATTGATGATATAACACTGGAAACTTCTCAGATCTTCGAGTTCCCGGATGTGTCAAAAAGGATATCCAGGATGGTTTCTGGAGCTGTCCCTCAATTTCAGAAGCTTCCTGATATCCGTCTAAGACCAGGTGATTTTGAATCCCTAAGTGGTAGAGAAAAGTCTCGCCATATAGGGTCAGCTCAGGGGCTCTTATACTCAATCTTAGTAGCAATTCACGACTCAGGATACAATGATGGGACCATCTTCCCTGTCAACATATACGGCAAAGTTTCCCCCAGAGACTATTTGAGAGGGCTTGCAAGAGGGATCTTGATAGGGTCCTCGATTTGCTTCTTGACACGAATGACAAATATTAACATTAACAGACCTCTTGAATTGATCTCAGGGGTAATTTCCTATATTCTCCTGAGGCTGGATAATCATCCCTCTCTGTATATAATGCTCAGAGAACCGTCTCTTAGAGGAGAAATATTCTCTATCCCTCAGAAAATCCCCGCCGCTTACCCAACCACTATGAGAGAAGGCAACAGATCGATTTTGTGTTACCTCCAACACGTGCTACGCTATGAGCGAGAGGCAATCACGGCGTCCCCGGAGAATGACTGGCTGTGGATCTTCTCAGACTTCAGAAGTGTGAAAATGACGTACTTGACCCTCATTACTTACCAGTCTCACCTCCTACTCCAGAGGGTTGAGAGAAACTTGTCTAAGAGTATGAGAGCTACTCTGCGACAAATGGGTTCCTTAATGAGGCAAGTGCTGGGTGGGCACGGAGAAGATACCTTGGAGTCAGACGATGACATTCAACGATTACTAAAAGACTCTTTGCGAAAGACAAGGTGGGTGGATCAAGAGGTGCGCCATGCAGCTAGAACCATGAATGGAGATTACAGCCCCGACAAGAAAGTATCCCGTAAGGTAGGATGTTCAGAATGGGTCTGCTCTGCTCAACAGATTGCCGTCTCCACCTCAGCCAACCCGGCCCCTGTCTCAGAGCTTGACATTAGGGCCCTCTCTAAGCGGTTTCAAAACCCCTTGATCTCGGGCCTGAGAGTGGTTCAGTGGGCAACCGGTGCCCATTATAAGCTTAAGCCTATTCTAGATGATCTAAATGTTTTCCCATCTCTCTGTCTTGTAATTGGAGACGGGTCAGGGGGAATATCAAGGGCAGTTCTCAACATGTTTCCAGATTCTAAGCTTGTGTTCAACAGCCTATTGGAGGTGAATGATCTGATGGCTTCCGGAACACATCCACTGCCTCCTTCAGCAATCATGAGTGGAGGAGATGACATCATCTCCAGAGTGATAGACTTTGACTCAATATGGGAGAAACCGTCCGACCTGAGGAACTCGGCCACCTGGAGATACTTCCAGTCGGTTCAAAAACAGGTCAACATGTCGTATGACCTCATTATTTGTGATGCAGAAGTTACTGATATTGCATCTATCAACCGGATAACTCTGTTGATGTCTGATTTCGCATTGTCTATAGATGGACCACTTTATCTGGTCTTCAAAACTTACGGGACTATGCTAGTAAACCCGGACTATAAAGCTATTCAACATCTGTCAAGAGCGTTCCCTTCGGTCACAGGGTTTGTAACCCAAGTAACTTCATCCTTTTCTTCTGAGCTATACCTCCGGTTCTCTAAACGAGGAAAGTTTTTCAGGGACGCCGAGTACTTGACCTCTTCCACCCTTCGAGAGATGAGCCTTGTGTTGTTCAATTGCAGCAGCCCCAAAAGTGAGATGCAGAGAGCTCGTTCCTTAAACTATCAAGACCTGGTAAGGGGATTTCCTGAAGAGATCATATCAAATCCTTACAACGAAATGATCATAACTCTGATTGACAATGATGTAGAGTCCTTCCTAGTCCACAAGATGGTGGATGATCTTGAGCTACAGAGGGGAACTCTGTCTAAAGTGGCTATCATTATATCCATCATGATCGTTTTTTCCAATAGAGTCTTCAACATTTCCAAACCTTTGACTGACCCCTTGTTCTATCCACCGTCTGATCCTAAAATCCTGAGGCACTTCAACATATGTTGCAGTACTATGATGTATCTATCTACCGCTTTAGGCGACGTCCCTAACTTCGCAAGACTTCATGACCTGTATAACAGACCTATAACTTGTTACTTCAGAAAACAAGTTATTCGAGGGAATATTTATCTATCTTGGAGTTGGTCCGATGATACCCCAGTGTTCAAGAGAGTAGCCTGTAATTCTAGCTTGAGTCTGTCATCTCACTGGATCAGGTTGATCTACAAGATAGTGAAGACTACCAGACTCATTGGCAGCATAAAAGACCTATCAGGAGAGGTAGAACGACATCTTCATGGGTATAACAGATGGATCACCCTCGAGGATATCCGATCTAGATCATCCCTACTAGATTACAGTTGTTTGTAAGCCGGATATTACCGAAAGCCTGTGCATGCTAAAATTCTTGTATGATGCATCTTGAAAAAAACAAGATCTTGAATCCGGACCTCTGGTTGTTTGATTGTTTTTTCCATCTTTATTGTTTTTTTGTTAAGCGTGGGTCGGCATGGCATCTCCACCTCCTCGCGGTCCGACCTGGGCATCCGAAGGAGGACGCACGTCCACTCGGATGGCTAAGGGAGGGGCGGGGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGTCGCGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTTCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAGCGCGCCTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCTCTGGCTAACTAGAGAACCCACTGCTTACTGGCTTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTGGCTAGATTAAGCGTCTGATGAGTCCGTGAGGACGAAACCCGGCGTACCGGGTC
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