EPR Plasmid #740: pAAV-CMV-H2B-3xFLAG-2A-G(N2c)
Sequence: download sequence
| Purpose of plasmid | AAV constitutively expressing nuclear FLAG and Rabies Glycoprotein (G) of CVS-N2c strain |
| Depositing Lab |
Marco Tripodi MRC Laboratory of Molecular Biology |
| Depositing Scientist | Fabio Morgese |
| Antibiotic Resistance | Ampicillin |
| Bacterial Growth Temp | 30 |
| Copy Number | High |
| Selectable Marker | |
| Reference |
Combining long-term circuit mapping and network transcriptomics with SiR-N2c. Lee H et al. Nat Methods 2023 PubMed DOI |
| MTA and Terms |
MTA (PDF download) Rabies CVS-N2c glycoprotein (G) |
How to cite this reagent:
In the Materials & Methods:
pAAV-CMV-H2B-3xFLAG-2A-G(N2c) was a gift from Marco Tripodi (European Plasmid Repository plasmid #740).
In the References:
Lee H et al.. Combining long-term circuit mapping and network transcriptomics with SiR-N2c.. Nat Methods 2023 doi:10.1038/s41592-023-01787-1 PubMed 36864202
pAAV-CMV-H2B-3xFLAG-2A-G(N2c) was a gift from Marco Tripodi (European Plasmid Repository plasmid #740).
In the References:
Lee H et al.. Combining long-term circuit mapping and network transcriptomics with SiR-N2c.. Nat Methods 2023 doi:10.1038/s41592-023-01787-1 PubMed 36864202
Sequence: download sequence
CCTGCAGGCAGCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCGGGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCCATCACTAGGGGTTCCTGCGGCCGCACGCGTGGAGCTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGTCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAGATCGCCTGGAGACGCCATCCACGCTGTTTTGACCTCCATAGAAGACACCGGGACCGATCCAGCCTCCGCGGATTCGAATCCCGGCCGGGAACGGTGCATTGGAACGCGGATTCCCCGTGCCAAGAGTGACGTAAGTACCGCCTATAGAGTCTATAGGCCCACAAAAAATGCTTTCTTCTTTTAATATACTTTTTTGTTTATCTTATTTCTAATACTTTCCCTAATCTCTTTCTTTCAGGGCAATAATGATACAATGTATCATGCCTCTTTGCACCATTCTAAAGAATAACAGTGATAATTTCTGGGTTAAGGCAATAGCAATATTTCTGCATATAAATATTTCTGCATATAAATTGTAACTGATGTAAGAGGTTTCATATTGCTAATAGCAGCTACAATCCAGCTACCATTCTGCTTTTATTTTATGGTTGGGATAAGGCTGGATTATTCTGAGTCCAAGCTAGGCCCTTTTGCTAATCATGTTCATACCTCTTATCTTCCTCCCACAGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTGGGATTCGAACATCGATTGAaTTCGGTACCCTTCAGGTCGACccaccATGCCAGAGCCAGCGAAGTCTGCTCCCGCCCCGAAAAAGGGCTCCAAGAAGGCGGTGACTAAGGCGCAGAAGAAAGGCGGCAAGAAGCGCAAGCGCAGCCGCAAGGAGAGCTATTCCATCTATGTGTACAAGGTTCTGAAGCAGGTCCACCCTGACACCGGCATTTCGTCCAAGGCCATGGGCATCATGAAcTCGTTTGTGAACGACATTTTCGAGCGCATCGCAGGTGAGGCTTCCCGCCTGGCGCATTACAACAAGCGCTCGACCATCACCTCCAGGGAGATCCAGACGGCCGTGCGCCTGCTGCTGCCTGGGGAGTTGGCCAAGCACGCCGTGTCCGAGGGTACTAAGGCCGTCACCAAGTACACCAGCGCTAAGGGATCTGGGGGTGGAGGCTCCGGCGGGGGTGGATCTGGAGGTGGGGGCAGCGGCGGAGGTGGGCTAGCGCTACCGCGGGCCACCGACTACAAAGACCATGACGGTGATTATAAAGATCATGACATCGACTACAAGGATGACGATGACAAGGGATCgGGAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGACGTGGAAGAAAACCCCGGTCCTATGGTGCCTCAGGTGCTGCTGTTTGTGCTGCTGCTGGGCTTCAGCCTGTGCTTCGGCAAGTTCCCCATCTACACCATCCCCGACGAGCTGGGCCCTTGGAGCCCTATCGATATCCACCACCTGAGCTGCCCCAACAACCTGGTGGTGGAAGATGAGGGCTGCACCAACCTGAGCGAGTTCAGCTACATGGAACTGAAAGTGGGCTACATCAGCGCCATCAAAGTGAACGGCTTCACCTGTACCGGCGTCGTGACCGAGGCCGAGACATACACCAACTTCGTGGGCTACGTGACCACCACCTTCAAGAGAAAGCACTTCAGACCCACCCCCGACGCCTGCAGAGCCGCCTACAATTGGAAGATGGCCGGCGACCCCAGATACGAGGAAAGCCTGCACAACCCCTACCCCGACTACCACTGGCTGAGAACCGTGCGGACCACCAAAGAGAGCCTGATCATCATCAGCCCCAGCGTGACCGACCTGGACCCCTACGACAAGAGCCTGCACTCCAGAGTGTTCCCTGGCGGCAAGTGCAGCGGCATCACCGTGTCTAGCACCTACTGCAGCACCAACCACGACTACACAATCTGGATGCCCGAGAACCCCAGACCCAGAACCCCCTGCGACATCTTCACCAACAGCAGAGGCAAGAGAGCCAGCAACGGCAACAAGACCTGCGGCTTCGTGGACGAGAGAGGCCTGTACAAGTCCCTGAAGGGCGCCTGCCGGCTGAAGCTGTGTGGCGTGCTGGGACTGAGACTGATGGACGGCACATGGGTGGCCATGCAGACCAGCGACGAGACAAAGTGGTGCCCCCCTGATCAGCTCGTGAACCTGCACGACTTCAGATCCGACGAGATCGAGCATCTGGTGGTGGAAGAACTCGTGAAGAAAAGGGAAGAGTGCCTGGACGCCCTGGAATCCATCATGACCACCAAGAGCGTGTCCTTCAGAAGGCTGAGCCACCTGAGAAAGCTGGTGCCCGGCTTTGGCAAGGCTTACACCATCTTCAACAAGACACTGATGGAAGCCGACGCCCACTACAAGTCTGTGCGGACCTGGAACGAGATCATCCCCAGCAAGGGCTGCCTGAAAGTGGGAGGCAGATGCCACCCTCACGTGAACGGCGTGTTCTTCAACGGCATCATCCTGGGCCCCGACGACCACGTGCTGATCCCTGAGATGCAGAGCAGCCTGCTGCAGCAGCATATGGAACTGCTGGAAAGCAGCGTGATCCCCCTGATGCACCCTCTGGCCGACCCTAGCACCGTGTTCAAAGAGGGCGACGAGGCTGAGGACTTCGTGGAAGTGCATCTGCCCGACGTGTACAAGCAGATCAGCGGCGTGGACCTGGGCCTGCCCAACTGGGGCAAATATGTGCTGATGACCGCTGGCGCCATGATCGGCCTGGTGCTGATCTTCTCCCTGATGACCTGGTGCAGAAGGGCCAACAGACCCGAGAGCAAGCAGAGAAGCTTCGGCGGCACAGGCGGCAACGTGTCCGTGACAAGCCAGAGCGGCAAAGTGATCCCTAGCTGGGAGAGCTACAAGAGCGGCGGAGAGATCAGACTGTGATGATGAAAGCTTGCCtggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaatcatcgtcctttccttggctgctcgcctatgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcatcgataccgagcgctgctcgagagatcgatctgcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggacacgtgcgGACCGAGCGGCCGCAGGAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTCAGTGAGCGAGCGAGCGCGCAGCTGCCTGCAGGGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATACGTCAAAGCAACCATAGTACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTTGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGGCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTTTATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGT
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