EPR Plasmid #58: pABM112-35S:VCS-GFP
Sequence: download sequence
| Purpose of plasmid | VCS-GFP Plant vector |
| Depositing Lab |
Alexis Maizel Heidelberg University |
| Depositing Scientist | Alexis Maizel |
| Antibiotic Resistance | Spectinomycin |
| Bacterial Growth Temp | 37 |
| Copy Number | High |
| Selectable Marker | |
| Reference |
Cytoplasmic and nuclear quality control and turnover of single-stranded RNA modulate post-transcriptional gene silencing in plants. Moreno AB et al. Nucleic Acids Res 2013 PubMed DOI |
| MTA and Terms |
MTA (PDF download) |
How to cite this reagent:
In the Materials & Methods:
pABM112-35S:VCS-GFP was a gift from Alexis Maizel (European Plasmid Repository plasmid #58).
In the References:
Moreno AB et al.. Cytoplasmic and nuclear quality control and turnover of single-stranded RNA modulate post-transcriptional gene silencing in plants.. Nucleic Acids Res 2013 doi:10.1093/nar/gkt152 PubMed 23482394
pABM112-35S:VCS-GFP was a gift from Alexis Maizel (European Plasmid Repository plasmid #58).
In the References:
Moreno AB et al.. Cytoplasmic and nuclear quality control and turnover of single-stranded RNA modulate post-transcriptional gene silencing in plants.. Nucleic Acids Res 2013 doi:10.1093/nar/gkt152 PubMed 23482394
Sequence: download sequence
GGCCCTCTAGAGGATCCCCGGGTACCCTCGAATTATCATACATGAGAATTAAGGGAGTCACGTTATGACCCCGCCGATGACGCGGGACAAGCCGTTTTACGTTTGGAACTGACAGAACCGCAACGTTGAAGGAGCCACTGAGCCGCGGGTTTCTGGAGTTTAATGAGCTAAGCACATACGTCAGAAACCATTATTGCGCGTTCAAAAGTCGCCTAAGGTCACTATCAGCTAGCAAATATTTCTTGTCAAAAATGCTCCACTGACGTTCCATAAATTCCCCTCGGTATCCAATTAGAGTCTCATATTCACTCTCAACTCGAATCCCCCCTATGAAAAAGCCTGAACTCACCGCGACGTCTGTCGAGAAGTTTCTGATCGAAAAGTTCGACAGCGTCTCCGACCTGATGCAGCTCTCGGAGGGCGAAGAATCTCGTGCTTTCAGCTTCGATGTAGGAGGGCGTGGATATGTCCTGCGGGTAAATAGCTGCGCCGATGGTTTCTACAAAGATCGTTATGTTTATCGGCACTTTGCATCGGCCGCGCTCCCGATTCCGGAAGTGCTTGACATTGGGGAGTTCAGCGAGAGCCTGACCTATTGCATCTCCCGCCGTGCACAGGGTGTCACGTTGCAAGACCTGCCTGAAACCGAACTGCCCGCTGTTCTTCAGCCGGTCGCGGAGGCTATGGATGCTATCGCTGCGGCCGATCTTAGCCAGACGAGCGGGTTCGGCCCATTCGGACCGCAAGGAATCGGTCAATACACTACATGGCGTGATTTCATATGCGCGATTGCTGATCCCCATGTGTATCACTGGCAAACTGTGATGGACGACACCGTCAGTGCGTCCGTCGCGCAGGCTCTCGATGAGCTGATGCTTTGGGCGAGGACTGCCCCGAAGTCCGGCACTCGTGCACGCGGATTCGGCTCCAACAATGTCCTGACGGACAATGGCCGCATAACAGCGGTCATTGACTGGAGCGAGGCGATGTTCGGGGATTCCCAATACGAGGTCGCCAACATCTTCTTCTGGAGGCCGTGGTTGGCTTGTATGGAGCAGCAGACGCGCTACTTCGAGCGGAGGCATCCGGAGCTTGCAGGATCGCCAAGGCTCCGGGCGTATATGCTCCGCATTGGTCTTGACCAACTCTATCAGAGCTTGGTTGACGGCAATTTCGATGATGCAGCTTGGGCGCAGGGTCGATGCGACGCAATCGTCCGATCCGGAGCCGGGACTGTCGGGCGTACACAAATCGCCCGCAGAAGCGCGGCCGTCTGGACCGATGGCTGTGTAGAAGTACTCGCCGATAGTGGAAACCGACGCCCCAGCACTCGTCCGAGGGCAAAGGAATAGAGTAGATGCCGACCGAACAAGAGCTGATTTCGAGAACGCCTCAGCCAGCAACTCGCGCGAGCCTAGCAAGGCAAATGCGAGAGAACGGCCTTACGCTTGGTGGCACAGTTCTCGTCCACAGTTCGCTAAGCTCGCTCGGCTGGGTCGCGGGAGGGCCGGTCGCAGTGATTCAGGAATTAATTCCCTAGAGTCAAGCAGATCGTTCAAACATTTGGCAATAAAGTTTCTTAAGATTGAATCCTGTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATGTAATGCATGACGTTATTTATGAGATGGGTTTTTATGATTAGAGTCCCGCAATTATACATTTAATACGCGATAGAAAACAAAATATAGCGCGCAAACTAGGATAAATTATCGCGCGCGGTGTCATCTATGTTACTAGATCGACCGGCATGCAAGCTGATAATTCAATTCGGCGTTAATTCAGTACATTAAAAACGTCCGCAATGTGTTATTAAGTTGTCTAAGCGTCAATTTGTTTACACCACAATATATCCTGCCACCAGCCAGCCAACAGCTCCCCGACCGGCAGCTCGGCACAAAATCACCACTCGATACAGGCAGCCCATCAGTCCGGGACGGCGTCAGCGGGAGAGCCGTTGTAAGGCGGCAGACTTTGCTCATGTTACCGATGCTATTCGGAAGAACGGCAACTAAGCTGCCGGGTTTGAAACACGGATGATCTCGCGGAGGGTAGCATGTTGATTGTAACGATGACAGAGCGTTGCTGCCTGTGATCAATTCGGGCACGAACCCAGTGGACATAAGCCTCGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTAACTGCCGTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTCTTGTTATGACATGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGGGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAATGTCTAGCTAGAAATTCGTTCAAGCCGACGCCGCTTCGCCGGCGTTAACTCAAGCGATTAGATGCACTAAGCACATAATTGCTCACAGCCAAACTATCAGGTCAAGTCTGCTTTTATTATTTTTAAGCGTGCATAATAAGCCCTACACAAATTGGGAGATATATCATGCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGGGTGCCTTGATGTGGGCGCCGGCGGTCGAGTGGCGACGGCGCGGCTTGTCCGCGCCCTGGTAGATTGCCTGGCCGTAGGCCAGCCATTTTTGAGCGGCCAGCGGCCGCGATAGGCCGACGCGAAGCGGCGGGGCGTAGGGAGCGCAGCGACCGAAGGGTAGGCGCTTTTTGCAGCTCTTCGGCTGTGCGCTGGCCAGACAGTTATGCACAGGCCAGGCGGGTTTTAAGAGTTTTAATAAGTTTTAAAGAGTTTTAGGCGGAAAAATCGCCTTTTTTCTCTTTTATATCAGTCACTTACATGTGTGACCGGTTCCCAATGTACGGCTTTGGGTTCCCAATGTACGGGTTCCGGTTCCCAATGTACGGCTTTGGGTTCCCAATGTACGTGCTATCCACAGGAAAGAGACCTTTTCGACCTTTTTCCCCTGCTAGGGCAATTTGCCCTAGCATCTGCTCCGTACATTAGGAACCGGCGGATGCTTCGCCCTCGATCAGGTTGCGGTAGCGCATGACTAGGATCGGGCCAGCCTGCCCCGCCTCCTCCTTCAAATCGTACTCCGGCAGGTCATTTGACCCGATCAGCTTGCGCACGGTGAAACAGAACTTCTTGAACTCTCCGGCGCTGCCACTGCGTTCGTAGATCGTCTTGAACAACCATCTGGCTTCTGCCTTGCCTGCGGCGCGGCGTGCCAGGCGGTAGAGAAAACGGCCGATGCCGGGATCGATCAAAAAGTAATCGGGGTGAACCGTCAGCACGTCCGGGTTCTTGCCTTCTGTGATCTCGCGGTACATCCAATCAGCTAGCTCGATCTCGATGTACTCCGGCCGCCCGGTTTCGCTCTTTACGATCTTGTAGCGGCTAATCAAGGCTTCACCCTCGGATACCGTCACCAGGCGGCCGTTCTTGGCCTTCTTCGTACGCTGCATGGCAACGTGCGTGGTGTTTAACCGAATGCAGGTTTCTACCAGGTCGTCTTTCTGCTTTCCGCCATCGGCTCGCCGGCAGAACTTGAGTACGTCCGCAACGTGTGGACGGAACACGCGGCCGGGCTTGTCTCCCTTCCCTTCCCGGTATCGGTTCATGGATTCGGTTAGATGGGAAACCGCCATCAGTACCAGGTCGTAATCCCACACACTGGCCATGCCGGCCGGCCCTGCGGAAACCTCTACGTGCCCGTCTGGAAGCTCGTAGCGGATCACCTCGCCAGCTCGTCGGTCACGCTTCGACAGACGGAAAACGGCCACGTCCATGATGCTGCGACTATCGCGGGTGCCCACGTCATAGAGCATCGGAACGAAAAAATCTGGTTGCTCGTCGCCCTTGGGCGGCTTCCTAATCGACGGCGCACCGGCTGCCGGCGGTTGCCGGGATTCTTTGCGGATTCGATCAGCGGCCGCTTGCCACGATTCACCGGGGCGTGCTTCTGCCTCGATGCGTTGCCGCTGGGCGGCCTGCGCGGCCTTCAACTTCTCCACCAGGTCATCACCCAGCGCCGCGCCGATTTGTACCGGGCCGGATGGTTTGCGACCGTCACGCCGATTCCTCGGGCTTGGGGGTTCCAGTGCCATTGCAGGGCCGGCAGACAACCCAGCCGCTTACGCCTGGCCAACCGCCCGTTCCTCCACACATGGGGCATTCCACGGCGTCGGTGCCTGGTTGTTCTTGATTTTCCATGCCGCCTCCTTTAGCCGCTAAAATTCATCTACTCATTTATTCATTTGCTCATTTACTCTGGTAGCTGCGCGATGTATTCAGATAGCAGCTCGGTAATGGTCTTGCCTTGGCGTACCGCGTACATCTTCAGCTTGGTGTGATCCTCCGCCGGCAACTGAAAGTTGACCCGCTTCATGGCTGGCGTGTCTGCCAGGCTGGCCAACGTTGCAGCCTTGCTGCTGCGTGCGCTCGGACGGCCGGCACTTAGCGTGTTTGTGCTTTTGCTCATTTTCTCTTTACCTCATTAACTCAAATGAGTTTTGATTTAATTTCAGCGGCCAGCGCCTGGACCTCGCGGGCAGCGTCGCCCTCGGGTTCTGATTCAAGAACGGTTGTGCCGGCGGCGGCAGTGCCTGGGTAGCTCACGCGCTGCGTGATACGGGACTCAAGAATGGGCAGCTCGTACCCGGCCAGCGCCTCGGCAACCTCACCGCCGATGCGCGTGCCTTTGATCGCCCGCGACACGACAAAGGCCGCTTGTAGCCTTCCATCCGTGACCTCAATGCGCTGCTTAACCAGCTCCACCAGGTCGGCGGTGGCCCATATGTCGTAAGGGCTTGGCTGCACCGGAATCAGCACGAAGTCGGCTGCCTTGATCGCGGACACAGCCAAGTCCGCCGCCTGGGGCGCTCCGTCGATCACTACGAAGTCGCGCCGGCCGATGGCCTTCACGTCGCGGTCAATCGTCGGGCGGTCGATGCCGACAACGGTTAGCGGTTGATCTTCCCGCACGGCCGCCCAATCGCGGGCACTGCCCTGGGGATCGGAATCGACTAACAGAACATCGGCCCCGGCGAGTTGCAGGGCGCGGGCTAGATGGGTTGCGATGGTCGTCTTGCCTGACCCGCCTTTCTGGTTAAGTACAGCGATAACCTTCATGCGTTCCCCTTGCGTATTTGTTTATTTACTCATCGCATCATATACGCAGCGACCGCATGACGCAAGCTGTTTTACTCAAATACACATCACCTTTTTAGACGGCGGCGCTCGGTTTCTTCAGCGGCCAAGCTGGCCGGCCAGGCCGCCAGCTTGGCATCAGACAAACCGGCCAGGATTTCATGCAGCCGCACGGTTGAGACGTGCGCGGGCGGCTCGAACACGTACCCGGCCGCGATCATCTCCGCCTCGATCTCTTCGGTAATGAAAAACGGTTCGTCCTGGCCGTCCTGGTGCGGTTTCATGCTTGTTCCTCTTGGCGTTCATTCTCGGCGGCCGCCAGGGCGTCGGCCTCGGTCAATGCGTCCTCACGGAAGGCACCGCGCCGCCTGGCCTCGGTGGGCGTCACTTCCTCGCTGCGCTCAAGTGCGCGGTACAGGGTCGAGCGATGCACGCCAAGCAGTGCAGCCGCCTCTTTCACGGTGCGGCCTTCCTGGTCGATCAGCTCGCGGGCGTGCGCGATCTGTGCCGGGGTGAGGGTAGGGCGGGGGCCAAACTTCACGCCTCGGGCCTTGGCGGCCTCGCGCCCGCTCCGGGTGCGGTCGATGATTAGGGAACGCTCGAACTCGGCAATGCCGGCGAACACGGTCAACACCATGCGGCCGGCCGGCGTGGTGGTGTCGGCCCACGGCTCTGCCAGGCTACGCAGGCCCGCGCCGGCCTCCTGGATGCGCTCGGCAATGTCCAGTAGGTCGCGGGTGCTGCGGGCCAGGCGGTCTAGCCTGGTCACTGTCACAACGTCGCCAGGGCGTAGGTGGTCAAGCATCCTGGCCAGCTCCGGGCGGTCGCGCCTGGTGCCGGTGATCTTCTCGGAAAACAGCTTGGTGCAGCCGGCCGCGTGCAGTTCGGCCCGTTGGTTGGTCAAGTCCTGGTCGTCGGTGCTGACGCGGGCATAGCCCAGCAGGCCAGCGGCGGCGCTCTTGTTCATGGCGTAATGTCTCCGGTTCTAGTCGCAAGTATTCTACTTTATGCGACTAAAACACGCGACAAGAAAACGCCAGGAAAAGGGCAGGGCGGCAGCCTGTCGCGTAACTTAGGACTTGTGCGACATGTCGTTTTCAGAAGACGGCTGCACTGAACGTCAGAAGCCGACTGCACTATAGCAGCGGAGGGGTTGGATCAAAGTACTTTGATCCCGAGGGGAACCCTGTGGTTGGCATGCACATACAAATGGACGAACGGATAAACCTTTTCACGCCCTTTTAAATATCCGTTATTCTAATAAACGCTCTTTTCTCTTAGGTTTACCCGCCAATATATCCTGTCAAACACTGATAGTTTAAACTGAAGGCGGGAAACGACAATCTGATCCAAGCTCAAGCTAAGCTTGAGCTCTCCCATATGGTCGACTAGAGCCAAGCTGATCTCCTTTGCCCCGGAGATCACCATGGACGACTTTCTCTATCTCTACGATCTAGGAAGAAAGTTCGACGGAGAAGGTGACGATACCATGTTCACCACCGATAATGAGAAGATTAGCCTCTTCAATTTCAGAAAGAATGCTGACCCACAGATGGTTAGAGAGGCCTACGCGGCAGGTCTCATCAAGACGATCTACCCGAGTAATAATCTCCAGGAGATCAAATACCTTCCCAAGAAGGTTAAAGATGCAGTCAAAAGATTCAGGACTAACTGCATCAAGAACACAGAGAAAGATATATTTCTCAAGATCAGAAGTACTATTCCAGTATGGACGATTCAAGGCTTGCTTCATAAACCAAGGCAAGTAATAGAGATTGGAGTCTCTAAGAAAGTAGTTCCTACTGAATCAAAGGCCATGGAGTCAAAAATTCAGATCGAGGATCTAACAGAACTCGCCGTGAAGACTGGCGAACAGTTCATACAGAGTCTTTTACGACTCAATGACAAGAAGAAAATCTTCGTCAACATGGTGGAGCACGACACTCTCGTCTACTCCAAGAATATCAAAGATACAGTCTCAGAAGACCAAAGGGCTATTGAGACTTTTCAACAAAGGGTAATATCGGGAAACCTCCTCGGATTCCATTGCCCAGCTATCTGTCACTTCATCAAAAGGACAGTAGAAAAGGAAGGTGGCACCTACAAATGCCATCATTGCGATAAAGGAAAGGCTATCGTTCAAGATGCCTCTGCCGACAGTGGTCCCAAAGATGGACCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGATATCTCCACTGACGTAAGGGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACTCCGGTATTTTTACAACAATACCACAACAAAACAAACAACAAACAACATTACAATTTACTATTCTAGTCGACCTGCAGGCGGCCGCACTAGTATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGGATATCACAAGTTTGTACAAAAAAGCAGGCTTTAAAGGAACCAATTCAGTCGACATGGCGTCTTCTCCTGGTAATACTAACCCTCACAATACTCCTCCGTTTGATCTCGGTATTCTCTTCAAACCTTCATCCAATCCTTATCCGCCACCGGCGGCATCTTATCCTCCGCCTACAGGTCCTTTTCTTCACAACCAGTACGATCAGCAGCACTACGCTCCGCCTGGTATCTCCGCTCAACCATCTCCGGTGACTCAGCAGCAACAGGATGTGTCTTCCTCTTCCGCCGCGACTAACTTGCATCCTCAGAGAACGCTGTCTTATCCCACGCCACCTCTCAATCTACAATCCCCCCGTTCCAATCACAATCCCGGTACGCACATCCTCGCTCTCCTTAACAATACCAACAACGGAGCCCCCGTGGCTAACCAAGAGCCGTCGCATCAGCTCCCAGTAGTCAATCACAACGAGATCGCTCGCTCTTTTCCCGGCGGTTCAGGTCCTATTCGTGTACCGAGCTGTAAATTGCCCAAGGGAAGGCGATTGATTGGTGAACACGCTGTGTACGATGTTGATGTGAGATTACAAGGTGAGATTCAGCCGCAGCTGGAGGTGACTCCGATTACCAAATACGGGTCGGATCCTCAGCTCGTAGTGGGTAGGCAAATCGCCGTGAATAAGGTTTACATTTGCTATGGATTGAAAGGAGGAAACATTAGGGTTCTCAATATCAACACAGCATTGAGGTCTCTGTTCCGTGGCCATTCACAGAGAGTGACGGATATGGCTTTCTTTGCCGAGGATGTTGATATGTTGGCCAGTGTTAGCCTAGATGGAAAAGTTTTTGTGTGGAAAATTTCTGAAGGGTCTGAGGGAGAGGATCAGCCCCAAATAACTGGGAAGATAGTACTTGCTCTTCAGATACTAGGAGAGGAAGACACCAAACATCCACGCGTTTGTTGGCACTGCCACAAACAGGAAATTTTGGTAGTTTCAATTGGTAAACATGTACTGCGTATTGATACTACAAAAGTTGGCAGAGGTGAAGTATTCTCTGCTGAGGCTCCTCTCCAGTGTCCCCTTGATAAGCTGATCGATGGTGTTCAGATTGTTGGTAAGCATGATGGAGAAGTGACAGATTTGTCAATGTGCCAATGGATGACCACGCGTCTGGTTTCTTCTTCAGTTGATGGCACGATTAAGATATGGCAAGATCGTAAGGCACAACCACTTGTAGTTTTGAGGCCTCATGATGGACATCCAGTCAGTTCAGCCACGTTTGTGACATCTCCTGAGAGACCTGATCACATCATACTTATCACGGGGGGTCCTCTAAATCGAGAAATGAAAATCTGGGTCTCTGCTGGGGAAGAAGGGTGGCTTTTACCAGCTGATGCTGAATCATGGAGGTGTACCCAGACTCTTGACTTGAAAAGTTCAACCGAGCCACGAGCTGAAGAGGCATTTTTTAACCAAGTCATAGCATTATCTGAAGCAGGCTTGCTTTTACTTGCAAATGCAAAAAGGAACGCCTTATATGCTGTGCATTTGGACTATGGTTCTTCTCCAGTTGGTACGCGGATGGATTACCTATCAGAGTTTACAGTCACTATGCCCATATTGAGTTTCATTGGGACAAATGATCCTCCAGAAGAACCCATTGTTAAGGTTTATTGTGTTCAGACTCTAGCAATCCAGCAGTATACATTAGACTTGTGCTTATGCTTGCCACCACCTATAGAAAATATGGGTTTGGAGAAGTCAGATTCTAGTGTATCACGAGAGGCAAATCTTGTTGAAGGCATGTCAGAACCATCTGGACTTAAACCTACTGACTTACCTTCAGTTGATTCAGTGCCAAAACCTTCGATTATAGTGAATAGATCAGAAAGTGCTAATAAGTTGAGTTTTCCATCAGCGGAAGCCACATCACAAGCAATTGTTCCACCCAACGGTGAACCTAAAACTTCTGGCCTGCCATCTCAAACCAGTGGTGCAGGTTCTGCGTATGCTACTTTACCCCAACTTCCTCTCAGTCCCAGACTATCAAGTAAACTTTCTGGCTATCACACTCCTGTAGAGGCGATTGAGCCAGTAATACCTCATCATGAGCTTGGTGGCAAAACACCTTCTGCTGATTACTCTGTTGATAGGCAAATGGATGCTGTTGGAGAAAGAAATTTGGACGTGTCATCCGTAGAAGAAATCTCAAGAAGCAAGGACTCAAATGTTACGCCTGATGATGATGTGTCTGGGATGCGAAGCCCATCAGCTTTTTTCAAACACCCCACTCATCTTGTAACTCCTTCAGAGATATTGATGGGCGTTTCTTCCGCTGAAGCCTCCATTACTACTGAAGACAGGAGAGATAGGGATGCAAATATTCAGGACGTGAATAATGATCCAAGAGACACCGAAGTAGAGGTGAAAGAAATAAGTGAAGCAAGGTCAACACAGAATGGTGAAATCAATGATCATGACGAAACTGAGAATTGCACTTCAGAAAATAGAGAAAAAGTCTTCTGCTCACAGGTTTCAAATCTCAGCACTGAGATGGCAAGAGACTGTTATCCTAGTACTGAGGGAACTTTCATTCCAGGGGAATCTAAGGCATATGGACAACCTATAAAGGCTGGAGATGAAAGTGGTGTTGACTCGAGAGGTGGGCCTGCAAAGCTTCTTAAAGGAAAGAAGCAGAAGGCCAAAAATTCACAGGGTCCCGGTTTGTCATCTACATCCTCAAATGTTGCTAATCTGGCTGACTCCTTCAATGAGCAAAGTCAGAGTTTAAGTCATCCCATGACAGATTTACTTCCTCAGTTGTTAGCAATGCAAGAAACGATGAATCAGGTGATGGCTTCGCAGAAGGAGATGCAGAGACAACTATCAAATGCTGCCACTGGCCCTATCGGAAAAGAAAGTAAAAGACTAGAAGTTGCGTTAGGGAGAATGATTGAGAAATCCAGCAAGTCAAATGCTGATGCTCTATGGGCCCGCATCCAGGAGGAGACTGTTAAGAATGAAAAGGCATTACGTGACCATGCCCAGCAAATTGTGAATGCAACGACAAACTTCATGAGCAAGGAGTTAAATGCCATGTTTGAGAAAACGATAAAGAAAGAATTGGCTGCAATTGGTCCAGCCCTAGCACGTTCAGTAGTACCAGTTATTGAAAAAACTGTATCTTCTGCAATCACAGAGTCCTTTCAGAGAGGAATAGGTGACAAAGCAGTTAATCAGCTTGACAAATCTGTTAATATAAAGCTTGAAGCAACCGTAGCTAGGCAAATTCAAGCCCAATTTCAGACCTCTGGCAAGCAAGCCCTCCAGGAAGGTCTTAGGTCAAGTGTGGAGTCCTCAGTCATACCTTCCTTTGAGAAGGCATGCAAGGCCATGTTTGACCAAATAGACTCAGCCTTCCAGAAAGGTATTGCTGAGCATACAAATGCAGCCCAGCAACGGTTTGACTCTGGACACTCCCAGCTTGCTCATACTCTAAAGGAATCAATTACTTCTGCGTCGTCAGTTGCTCAAGCCTTAAGTCGTGAGTTAGCCGAGACCCAAAGGAATCTCTTAGCTCTCGCAGCTGCTGGAGCAAATTCTGGTGGGTCTAATTCCTTGGTTACTCAACTAAGTGGCGGACCTTTGGGTGCTCTTCTTGAAAAGGTTGAAGCACCTATGGACCCAACAACAGAACTATCGAGGTTGATATCTGAACGGAAGTACGAAGAATCTTTCACTTCGGCCCTACAGAGAAGCGATGTCTCTATAGTATCATGGCTTTGCTCACAGGTGGATCTACGTGGATTACTGGCGATGAATCCGCTTCCACTGAGCCAAGGCGTGCTTCTTTCACTGCTGCAGCAGCTAGCCTGTGACATTAGCAAGGACACATCCCGTAAGCTGGCTTGGATGACTGATGTGGTTGCAGCCATAAACCCATCAGACCAGATGATTGCGGTCCACGCTCGGCCAATCTTTGAACAGGTATATCAGATTCTGCACCATCACCGCAACGCACCGGGAAGCGATGTCTCAGCCATCAGACTGATAATGCACGTGATCAACTCCATGCTTATGGGTTGCAAAKGAGCAAAGGAACCAATTCAGGGCCGCACTCGAGATATCTAGACCCAGCTTTCTTGTACAAAGTGGTGATATCCCGCGGCCATGCTAGAGTCCGCAAAAATCACCAGTCTCTCTCTACAAATCTATCTCTCTCTATTTTTCTCCAGAATAATGTGTGAGTAGTTCCCAGATAAGGGAATTAGGGTTCTTATAGGGTTTCGCTCATGTGTTGAGCATATAAGAAACCCTTAGTATGTATTTGTATTTGTAAAATACTTCTATCAATAAAATTTCTAATTCCTAAAACCAAAATCCAGTGACCTGCAGGCATGCGACGTCG
next plasmid