EPR Plasmid #566: GA06_CMV-Mb3Cas12a-hU6-BbsI-CMV-GFP
Sequence: download sequence
| Purpose of plasmid | Expression of Mb3Cas12a and one gRNA under a hU6 promoter. BbsI entry site to incorporate gRNA. Expression as eGFP as reporter. |
| Depositing Lab |
Ana Martin-Villalba Deutsches Krebsforschungszentrum (DKFZ) |
| Depositing Scientist | Katrin Volk |
| Antibiotic Resistance | Ampicillin |
| Bacterial Growth Temp | 37 |
| Copy Number | High |
| Selectable Marker | |
| Reference | This plasmid is from an unpublished project. Please acknowledge the depositing scientist. |
| MTA and Terms |
MTA (PDF download) |
How to cite this reagent:
In the Materials & Methods:
GA06_CMV-Mb3Cas12a-hU6-BbsI-CMV-GFP was a gift from Ana Martin-Villalba (European Plasmid Repository plasmid #566).
In the References:
No reference available. (This plasmid is not yet published.)
GA06_CMV-Mb3Cas12a-hU6-BbsI-CMV-GFP was a gift from Ana Martin-Villalba (European Plasmid Repository plasmid #566).
In the References:
No reference available. (This plasmid is not yet published.)
Sequence: download sequence
cctagagctcgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagagaatagcaggcatgctggggagcggccgccgcgttgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctctggctaactagaGAACCCACTGCGCCGCCACCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAAgagcagacatgataagatacattgatgagtttggacaaaccacaactagaatgcagtgaaaaaaatgctttatttgtgaaatttgtgatgctattgctttatttgtaaccattataagctgcaataaacaagttaacaacaacaattgcattcattttatgtttcaggttcagggggaggtgtgggaggttttttaaagcaagtaaaacctctacaaatgtggtagcggccgcaggaacccctagtgatggagttggccactccctctctgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcagctgcctgcaggggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatacgtcaaagcaaccatagtacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccttagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaactctatctcgggctattcttttgatttataagggattttgccgatttcggtctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaattttatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtggaagccgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcagctagcGTCATCATTAGTGTgTAcATGCCACCCCGAAAATACCGCCTAGAAATGTCTATGATTGAAAACGACTGCTAAATCCGCGTGACTCGAGgagggcctatttcccatgattccttcatatttgcatatacgatacaaggctgttagagagataattggaattaatttgactgtaaacacaaagatattagtacaaaatacgtgacgtagaaagtaataatttcttgggtagtttgcagttttaaaattatgttttaaaatggactatcatatgcttaccgtaacttgaaagtatttcgatttcttggctttatatatcttgtggaaaggacgaaacaccgggtcttcgagaagacctTTTTTTTTTATCTCTCCGTCCGAGCACGATAGAGAACgccgcgttgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctcctctggctaactaccggtcGCCACCatgctgttccaggacttcacccacctgtaccccctgagcaagaccgtcagattcgagctgaagcccatcggcaagaccctggaacacatccacgccaagaactttctgaaccaggacgagacaatggccgacatgtaccagaaagtgaaggccatcctggacgactaccaccgggactttatcgccgacatgatgggcgaagtgaagctgaccaagctggccgagttctacgacgtgtacctgaagttccggaagaaccccaaggacgacggcctgcagaagcagctgaaagacctgcaggctgtgctgcggaaagaaatcgtgaagcctatcggaaacggcggcaagtacaaggccggctacgacagactgttcggcgccaagctgttcaaggacggcaaagagctgggcgacctggccaagttcgtgatcgcccaggaaggcgagagcagccctaagctggcccacctggcccatttcgagaagttcagcacctacttcaccggcttccacgacaaccgcaagaacatgtacagcgacgaggacaagcacaccgccattgcctaccggctgatccacgagaacctgccccggttcatcgacaacctgcagatcctggccaccatcaagcagaagcacagcgccctgtacgaccagatcatcaacgagctgaccgccagcggcctggatgtgtctctggcctctcacctggatggctaccacaagctgctgacacaggaaggcatcaccgcctacaacaccctgctgggcggaatctctggcgaggccggcagcagaaagatccagggcatcaatgaactgatcaacagccaccacaaccagcactgccacaagagcgagcggattgccaagctgcggcccctgcacaagcagattctgagcgacggaatgggcgtgtccttcctgcccagcaagttcgccgacgacagcgaagtgtgccaggccgtgaatgagttctaccggcactacgccgacgtgttcgccaaggtgcagagcctgttcgacggcttcgacgactatcagaaagacggcatctacgtggagtacaagaacctgaacgagctgtccaagcaggccttcggcgacttcgctctgctgggaagagtgctggacggctactatgtggacgtcgtgaaccccgagttcaacgagagatttgccaaggccaagaccgacaacgccaaagccaagctgacaaaagagaaggacaagttcatcaagggcgtgcactccctggcttctctggaacaggccatcgagcactacaccgccagacacgacgacgagtctgtgcaggccggaaagctgggccagtacttcaagcacggactggccggcgtggacaaccccatccagaagatccacaacaaccactctacaatcaagggcttcctggaaagagagcggcctgccggcgaaagagccctgcccaagatcaagagcgacaagagccccgagatcagacagctgaaagagctgctggacaacgccctgaacgtggcccacttcgccaaactgctgaccaccaagaccacactgcacaatcaggatggcaacttctacggcgagttcggagccctgtatgatgagctggccaagatcgccaccctgtacaacaaagtgcgggactacctgagccagaagcccttcagcaccgagaagtacaagctgaatttcggcaaccctaccctgctgaacggctgggacctgaacaaagagaaagataacttcggcgtgatcctgcagaaggacggctgctactacctggccctgctggataaggcccacaagaaggtgttcgataacgcccccaacaccggcaagtctgtgtatcagaagatgatctataagctgctgcctggccccaacaagatgctgcccaaggtgttcttcgccaagagcaacctggactactacaaccccagcgccgaactgctggacaaatacgcccagggcacacacaagaaaggcgacaacttcaacctgaaggactgccacgccctgatcgatttcttcaaggccggaatcaacaagcaccccgagtggcagcacttcggcttcaagttcagccccaccagcagctaccaggacctgagcgacttctacagagaggtggaaccccagggctaccaagtgaagttcgtggacatcaacgccgactacatcaatgagctggtggaacagggccagctgtacctgtttcagatctacaacaaggactttagccccaaggcccacggcaagcccaacctgcacaccctgtatttcaaggccctgtttagcgaggacaacctcgtgaatcccatctacaaactgaacggggaggccgagatcttctaccggaaggcctccctggacatgaacgagacaaccatccacagagccggcgaggtgctggaaaacaagaaccctgacaaccctaagaaacggcagttcgtgtacgacatcatcaaggacaagcggtacacccaggataagttcatgctgcacgtgcccatcaccatgaacttcggagtgcagggcatgaccatcaaagagttcaacaaaaaagtgaaccagagcatccagcagtacgacgaagtgaacgtgatcggcatcgaccggggcgagcggcatctgctgtatctgaccgtgatcaactccaagggcgagattctggaacagagatccctgaacgacatcaccaccgcctccgccaacggcacccagatgaccaccccttaccacaagatcctggacaagcgcgagatcgagcggctgaacgccagagtgggatggggagagatcgagacaatcaaagaactgaagtccggctacctgtcccacgtggtgcatcagatctcccagctgatgctgaagtacaacgccatcgtggtgctggaagatctgaattttggcttcaagaggggccggttcaaggtggaaaagcagatctaccagaacttcgagaatgccctgatcaagaaactgaaccacctggtgctgaaagacaaggccgacgacgagatcggcagctacaagaacgccctgcagctgactaacaacttcaccgatctgaagagtatcggcaagcagaccggctttctgttctacgtgcccgcctggaataccagcaagatcgaccccgagacaggcttcgtggacctgctgaagcctagatacgagaatatcgcccagagccaggccttcttcggcaagttcgacaagatctgctacaacgccgataggggctacttcgagttccacatcgactacgccaagttcaatgacaaggccaaaaacagccggcagatctggaaaatctgcagccacggcgataagcgctacgtgtacgataagaccgccaaccagaacaagggcgccaccatcggagtgaatgtgaacgatgagctgaagtccctgttcacccgctaccacatcaacgataagcagcccaatctcgtgatggacatctgccagaacaacgacaaagagtttcacaagagcctgatgtatctgctgaaaacactgctggctctgcggtacagcaacgccagctccgacgaggatttcatcctgagccccgtggccaacgacgagggcgtgttcttcaatagcgccctggccgacgatacccagccccagaatgccgatgccaacggcgcctaccacattgccctgaagggactgtggctgctgaacgaactgaagaacagcgacgatctgaacaaagtgaagctggccatcgacaaccagacctggctgaatttcgcccagaacagaaagaggccggcggccacgaagaaggccggccaggccaagaagaagaagggatcctacccatacgatgttccagattacgcttatccctacgacgtgcctgattatgcatacccatatgatgtccccgactatgcctaa
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