EPR Plasmid #457: pAP27
Sequence: download sequence
| Purpose of plasmid | Generation of Knock-In donors containing mNeonGreen (Linker::3xFlag::mNeonGreen::HAtag::Linker). Insert in pUC19, no expression sequence |
| Depositing Lab |
Alexandre Paix European Molecular Biology Laboratory (EMBL) |
| Depositing Scientist | Alexandre Paix |
| Antibiotic Resistance | Ampicillin |
| Bacterial Growth Temp | 37 |
| Copy Number | High |
| Selectable Marker | |
| Reference |
Endogenous protein tagging in medaka using a simplified CRISPR/Cas9 knock-in approach. Seleit A et al. Elife 2021 PubMed DOI |
| MTA and Terms |
MTA (PDF download) |
How to cite this reagent:
In the Materials & Methods:
pAP27 was a gift from Alexandre Paix (European Plasmid Repository plasmid #457).
In the References:
Seleit A et al.. Endogenous protein tagging in medaka using a simplified CRISPR/Cas9 knock-in approach.. Elife 2021 doi:10.7554/eLife.75050 PubMed 34870593
pAP27 was a gift from Alexandre Paix (European Plasmid Repository plasmid #457).
In the References:
Seleit A et al.. Endogenous protein tagging in medaka using a simplified CRISPR/Cas9 knock-in approach.. Elife 2021 doi:10.7554/eLife.75050 PubMed 34870593
Sequence: download sequence
TCTAGCGGACCTTCAGGTAGTTCGAGCGACTACAAGGACCACGACGGGGATTATAAAGATCATGATATAGATTATAAAGATGATGATGATAAAGTCTCCAAAGGGGAGGAAGACAACATGGCGTCCCTCCCAGCGACGCACGAACTCCATATCTTTGGGTCCATAAACGGTGTCGATTTCGACATGGTAGGACAAGGGACCGGAAATCCAAATGATGGATATGAGGAGCTAAACCTCAAGAGTACCAAGGGGGACCTCCAATTTTCCCCATGGATACTCGTCCCCCATATCGGGTACGGATTCCATCAATACCTACCATATCCAGATGGGATGTCCCCATTTCAAGCGGCCATGGTAGACGGAAGTGGATACCAAGTACATCGTACGATGCAATTTGAGGACGGGGCCTCCCTAACCGTCAACTATCGTTACACGTACGAGGGAAGTCACATCAAAGGAGAGGCGCAAGTCAAAGGAACTGGATTTCCAGCCGATGGACCAGTCATGACGAATTCCCTAACCGCCGCCGATTGGTGTCGTAGTAAAAAGACGTACCCAAATGACAAGACCATAATCTCCACGTTCAAGTGGTCCTATACCACCGGGAACGGAAAGCGGTACCGTAGTACGGCCCGGACGACGTACACGTTCGCCAAGCCCATGGCCGCTAATTACCTGAAGAATCAGCCAATGTATGTCTTCCGTAAAACCGAGCTAAAGCACTCCAAAACCGAGCTAAACTTCAAGGAATGGCAAAAGGCGTTCACGGACGTAATGGGAATGGACGAACTCTACAAGTACCCATATGATGTCCCGGATTACGCTAGCTCTGGTCCAAGTGGATCAAGCTCG
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