EPR Plasmid #443: Tol2(HSE:mCherrry-Cry2olig-zf_caspase-8_cmlc2:tagRFP)
Sequence: download sequence
| Purpose of plasmid | Expression of mCherry-Cry2olig-zf_caspase-8 under heat shock promoter, contains Tol2 sites for genome integration and cmlc2:tagRFP for selection |
| Depositing Lab |
Maria Leptin European Molecular Biology Laboratory (EMBL) |
| Depositing Scientist | Eva Hasel de Carvalho |
| Antibiotic Resistance | Ampicillin |
| Bacterial Growth Temp | 37 |
| Copy Number | High |
| Selectable Marker | |
| Retransformation Info: | Retransforms well in XL1blue. |
| Reference |
Optogenetic activators of apoptosis, necroptosis, and pyroptosis. Shkarina K et al. J Cell Biol 2022 PubMed DOI |
| MTA and Terms |
MTA (PDF download) |
How to cite this reagent:
In the Materials & Methods:
Tol2(HSE:mCherrry-Cry2olig-zf_caspase-8_cmlc2:tagRFP) was a gift from Maria Leptin (European Plasmid Repository plasmid #443).
In the References:
Shkarina K et al.. Optogenetic activators of apoptosis, necroptosis, and pyroptosis.. J Cell Biol 2022 doi:10.1083/jcb.202109038 PubMed 35420640
Tol2(HSE:mCherrry-Cry2olig-zf_caspase-8_cmlc2:tagRFP) was a gift from Maria Leptin (European Plasmid Repository plasmid #443).
In the References:
Shkarina K et al.. Optogenetic activators of apoptosis, necroptosis, and pyroptosis.. J Cell Biol 2022 doi:10.1083/jcb.202109038 PubMed 35420640
Sequence: download sequence
ATCACTAGTGACTGACTAGGATCTGGTTACCACTAAACCAGCCTCAAGAACACCCGAATGGAGTCTCTAAGCTACATAATACCAACTTACACTTTACAAAATGTTGTCCCCCAAAATGTAGCCATTCGTATCTGCTCCTAATAAAAAGAAAGTTTCTTCACATTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCGTAGATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAATTCGCGGCCGCCACCGCGGTGGAGCTCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGCGCNNNNNNNNNNNNNNNNNNNNNNNNGGTACCATCGATGATGATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTATGGCTGATTATGATCCTCTAGATCAGATCTCTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGGATCTACGTATCAATTAAGTTTGTGCCCCAGTTTGCTAGGGAGGTCGCAGTATCTGGCCACAGCCACCTCGTGCTGCTCGACGTAGGTCTCTTTGTCGGCCTCCTTGATTCTTTCCAGTCTGTGGTCCACATAGTAGACGCCGGGCATCTTGAGGTTCTTAGCGGGTTTCTTGGATCTGTATGTGGTCTTGAAGTTGCAGATCAGGTGGCCCCCGCCCACGAGCTTCAGGGCCATGTCGCTTCTGCCTTCCAGGCCGCCGTCAGCGGGGTACAGCATCTCGGTGTTGGCCTCCCAGCCGAGTGTTTTCTTCTGCATCACAGGGCCGTTGGATGGGAAGTTCACCCCTCTGATCTTGACGTTGTAGATGAGGCAGCCGTCCTGGAGGCTGGTGTCCTGGGTAGCGGTCAGCACGCCCCCGTCTTCGTATGTGGTGACTCTCTCCCATGTGAAGCCCTCAGGGAAGGACTGCTTAAAGAAGTCGGGGATGCCCTGGGTGTGGTTGATGAAGGTTCTGCTGCCGTACATGAAGCTGGTAGCCAGGATGTCGAAGGCGAAGGGGAGAGGGCCGCCCTCGACCACCTTGATTCTCATGGTCTGGGTGCCCTCGTAGGGCTTGCCTTCGCCCTCGGATGTGCACTTGAAGTGGTGGTTGTTCACGGTGCCCTCCATGTACAGCTTCATGTGCATGTTCTCCTTAATCAGCTCTTCGCCCTTAGACACCATGGTCACTGTCTGCTTTGCTGTTGGTCTGGGCTCCTGGGTCACTGGCTTACTAATGGAGTCTTTATGTATGAGGACTCTTATCAATTGTTCTTCTATAAAGGTCTGCAGTGTTTCTGTTCGTCCCCTACATGGACACCCAGAGCCTCCTAAATACAGGAGCCCTGATAACTGCACAAGTGCTCAGATTCCAGCAGGGTGGAAAATGAGATAAAGTGTGCAGATGGGGAGGGGGACGTGAATGAGAGATTTGAGGGATGAAAAGGATGGATGAACGCATTGAAAATAGCCCCTTTCACAAAATAATACCAGTAAATTGTCATATAATTAATGACCAGACCTTTACCTGTAAATGATGTGATGTTCATATACAGAAGAATGCCAACAATTTACAGATGATTTTACAAATTCTTAATTTTCCTTTTCTAAATGGATTTGTTCACACATTATCTCTTCAAAGCAATTGAATTTTCTGGAAAAGATTGGCTGTGTGTGTAAAAGGTGCTACTGAGGAATTTAATGTGACATGGAATGAAGCAAACAGCAGCCTAAGACAGGATGGCAGGAAAAATGTCACATGTTTAAAATAAGAGTGAAACCAACGACCTGGACTTATAGAGACTGTGTGTCCTTAGGCAAAGCTCTTGTACAAACAGTGCTTGTGACTGTGAAATTCGGGGTTTGCCTGGATTGTGTTAAATGTGTGCTTGGATGTATCATAAAAAAGAATCGGTTCTCTGTATTTGACATTTTCTGTAATTTATCTAAAGTTACAACTGGCTATGCCTGATTTTTATCACTATTGAATCAATCATTTTAAAGAATGTTAAGTCTGCTGATTTGGAGAGCTCATTTACATTTATTTTGAATGTCTCTTATTTAACACAACTGATTTAAGCTTTAGATCTGCGAAGATACGGCCACGGGTGCTCTTGATCCTGTGGCTGATTTTGGACTGTGCTGCTCGCAGCTGCTGATGAATCACATACTTCCTCCATTTTCTTCCACTGATTGACTGTTATAATTTCCCTAATTTCCAGGTCAAGGTGCTGTGCATTGTGGTAATAGATGTGACATGACGTCACTTCCAAAGGACCAATGAACATGTCTGACCAATTTCATATAATGTGAAAACGATTTTCATAGGCAGAATAAATAACATTTAAATTAAACTGGGCATCAGCGCAATTCAATTGGTTTGGTAATAGCAAGGGAAAATAGAATGAAGTGATCTCCAAAAAATAAGTACTTTTTGACTGTAAATAAAATTGTAAGGAGTAAAAAGTACTTTTTTTTCTAAAAAAATGTAATTAAGTAAAAGTAAAAGTATTGATTTTTAATTGTACTCAAGTAAAGTAAAAATCCCCAAAAATAATACTTAAGTACAGTAATCAAGTAAAATTACTCAAGTACTTTACACCTCTGGTTCTTGACCCCCTACCTTCAGCAAGCCCAGCAGATCCACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGCGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACGCTGGCTAAGAACTCATCAGCCTCCCCGGTCCATCTACCCACGTACCAATGCACCAATTGGCCACAATGACGGCTACTACATGGTGCCATTCCTTCCTCTTTATAGGAATGGAGACTACCTCCTGTCCAACAAGGCTCTTGGATACGAGTACGCCTACCTGTTGGACCCAGGTCATTGCACAACACCAGAAATGCCCTCTGATCTGCAAAAGACGTGAATATCTGTTCAGACACCCATATCCACTCTGTTCCACACAGGTCAGAGGTTTGTCCAGGAGTTCTTGACAGAGGTGTAAAAAGTACTCAAAAATTTTACTCAAGTGAAAGTACAAGTACTTAGGGAAAATTTTACTCAATTAAAAGTAAAAGTATCTGGCTAGAATCTTACTTGAGTAAAAGTAAAAAAGTACTCCATTAAAATTGTACTTGAGTATTAAGGAAGTAAAAGTAAAAGCAAGAAAGAAAACTAGAGATTCTTGTTTAAGCTTTTAATCTCAAAAAACATTAAATGAAATGCATACAAGGTTTTATCCTGCTTTAGAACTGTTTGTATTTAATTATCAAACTATAAGACAGACAATCTAATGCCAGTACACGCTACTCAAAGTTGTAAAACCTCAGATTTAACTTCAGTAGAAGCTGATTCTCAAAATTGTTAGTGTCAAGCCTAGCTCTTTTGGGGCTGAAAAGCAATCCTGCAGTGCTGAAAAGCCTCTCACAGGCAGCCGATGCGGGAAGAGGTGTATTAGTCTTGATAGAGAGGCTGCAAATAGCAGGAAACGTGAGCAGAGACTCCCTGGTGTCTGAAACACAGGCCAGATGGGCCCTCGAGCAGGAAACAGCTATGACCATGATTACGCCAAGCTATCAACTTTGTATAGAAAAGTTGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATCCAGTCACTATGGTCGACCTCGAAACGTTCTAGAACTGGAGAACGTTCTAGAACGTCGAGAACGTTCTAGAACTGGAGAACGTTCTAGAACGTCGAGAACGTTCTAGAACTGGAGAACGTTCTAGAACGTCGAGAACGTTCTAGAACTGGAGAACGTTCTAGAACGTCGAGGGGGGGCCCGCGTGAGCTCGGTACGGGTCGAGGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCTGGTTTAGTGAACCCTCATTCTGCAGTAATACGACTCACTATAGGGCTAGCTTGATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGGATCAGCTTGCTTGTTCTTTTTGCAGAAGCTCAGAATAAACGCTCAACTTTGGCAGATCTATCGATAAGCTTGAATTCGGATCCATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGCAGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGGGAGGAGGAGGAATGATGAAGATGGACAAAAAGACTATAGTTTGGTTTAGAAGAGACCTAAGGATTGAGGATAATCCTGCATTAGCAGCAGCTGCTCACGAAGGATCTGTTTTTCCTGTCTTCATTTGGTGTCCTGAAGAAGAAGGACAGTTTTATCCTGGAAGAGCTTCAAGATGGTGGATGAAACAATCACTTGCTCACTTATCTCAATCCTTGAAGGCTCTTGGATCTGACCTCACTTTAATCAAAACCCACAACACGATTTCAGCGATCTTGGATTGTATCCGCGTTACCGGTGCTACAAAAGTCGTCTTTAACCACCTCTATGATCCTGTTTCGTTAGTTCGGGACCATACCGTAAAGGAGAAGCTGGTGGAACGTGGGATCTCTGTGCAAAGCTACAATGGAGATCTATTGTATGAACCGTGGGAGATATACTGCGAAAAGGGCAAACCTTTTACGAGTTTCAATTCTTACTGGAAGAAATGCTTAGATATGTCGATTGAATCCGTTATGCTTCCTCCTCCTTGGCGGTTGATGCCAATAACTGCAGCGGCTGAAGCGATTTGGGCGTGTTCGATTGAAGAACTAGGGCTGGAGAATGAGGCCGAGAAACCGAGCAATGCGTTGTTAACTAGAGCTTGGTCTCCAGGATGGAGCAATGCTGATAAGTTACTAAATGAGTTCATCGAGAAGCAGTTGATAGATTATGCAAAGAACAGCAAGAAAGTTGTTGGGAATTCTACTTCACTACTTTCTCCGTATCTCCATTTCGGGGAAATAAGCGTCAGACACGTTTTCCAGTGTGCCCGGATGAAACAAATTATATGGGCAAGAGATAAGAACAGTGAAGGAGAAGAAAGTGCAGATCTTTTTCTTAGGGGAATCGGTTTAAGAGAGTATTCTCGGTATATATGTTTCAACTTCCCGTTTACTCACGAGCAATCGTTGTTGAGTCATCTTCGGTTTTTCCCTTGGGATGCTGATGTTGATAAGTTCAAGGCCTGGAGACAAGGCAGGACCGGTTATCCGTTGGTGGATGCCGGAATGAGAGAGCTTTGGGCTACCGGATGGATGCATAACAGAATAAGAGTGATTGTTTCAAGCTTTGCTGTGAAGTTTCTTCTCCTTCCATGGAAATGGGGAATGAAGTATTTCTGGGATACACTTTTGGATGCTGATTTGGAATGTGACATCCTTGGCTGGCAGTATATCTCTGGGAGTATCCCCGATGGCCACGAGCTTGATCGCTTGGACAATCCCGCGTTACAAGGCGCCAAATATGACCCAGAAGGTGAGTACATAAGGCAATGGCTTCCCGAGCTTGCGAGATTGCCAACTGAATGGATCCATCATCCATGGGACGCTCCTTTAACCGTACTCAAAGCTTCTGGTGTGGAACTCGGAACAAACTATGCGAAACCCATTGTAGACATCGACACAGCTCGTGAGCTACTAGCTAAAGCTATTTCAAGAACCCGTGGAGCACAGATCATGATCGGAGCAGCAGCCCGGGATCCACCGGTCGCCACCCAGGAATCAGAGTCACAGAGACCAAGAACAAGGAGGCAGACTTCCTCTAGAAGAAGTTTTTCTCAACAACCCTGTATCTGAAACAATGGAGAGAGAAAGGAGGAGAAACTCTTCGGCTGGCACAATTACTACAGACGCAGAAACTCCATTAAATCCAAATGAATATTACATACTGACCCAGCGGCCTCTTGGATACTGTCTAATCATCAACAACTGCAACTTCTTGAAAAGTACTAACCTGCTCAAACGAACAGGCACTGATATGGACAAAGATAGACTTGCCAAATTGTTTAGCAGAATGCATTTCCAGATTGAGGTACGGAATGATCTGGAAGCCTGGGCTATAAAAGATGAGATAAAGCAGTTTGCAAATAGGAATCATGCTTCAATGGGTGCTTTTGTTTGCTGTATTCTCTCACACGGAGAGAAGGGCACAGTTTTGGGCACAGATGGTAAACCAGTTGAAATCCGAGAAGTCACACTGCCTTTTGCCGGATGCAGAACACTAGCAAGCAAGCCCAAGCTTTTCTTTATTCAAGCCTGCCAAGGAGATGAGAACCAGGCTGGTGTTTGGACGTCGGATGGACGCGAGGATGCTCCTGAGGAAGACGAAAAATATGAGGAGGATGCAGGAATAATAGTGCTCCGCAAAATTCCAATCGAAGCTGATTTCCTGATCGGAATGGCCACAGTAGAGCACTACCTCTCCTACAGACACACTAAAGAGGGCTCTATCTTTATCCAGGAGCTCTGTAAAAAGATGGAGGAATTGTGTCCCAAGAAAGAGGACATGTTGTCCATTCTGACAAAGGTGAACTTTGAAGTGAGCAAAAGGATTTTGAAGGGCTACAAGCAGATGCCAGAACCACGCTACACGCTGACCAAGAAACTAGTGCTGCCCATAGACTAAGAT
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