EPR Plasmid #433: Tol2(HSE:zASC_Y152F-mKate2_cmlc2:tagRFP)
Sequence: download sequence
| Purpose of plasmid | Expression of zebrafish Y152F mutant variant of ASC under heatshock promter, Tol2 based genome integration |
| Depositing Lab |
Maria Leptin European Molecular Biology Laboratory (EMBL) |
| Depositing Scientist | Eva Hasel de Carvalho |
| Antibiotic Resistance | Ampicillin |
| Bacterial Growth Temp | 37 |
| Copy Number | High |
| Selectable Marker | |
| Reference |
Dynamics of in vivo ASC speck formation. Kuri P et al. J Cell Biol 2017 PubMed DOI |
| MTA and Terms |
MTA (PDF download) |
How to cite this reagent:
In the Materials & Methods:
Tol2(HSE:zASC_Y152F-mKate2_cmlc2:tagRFP) was a gift from Maria Leptin (European Plasmid Repository plasmid #433).
In the References:
Kuri P et al.. Dynamics of in vivo ASC speck formation.. J Cell Biol 2017 doi:10.1083/jcb.201703103 PubMed 28701426
Tol2(HSE:zASC_Y152F-mKate2_cmlc2:tagRFP) was a gift from Maria Leptin (European Plasmid Repository plasmid #433).
In the References:
Kuri P et al.. Dynamics of in vivo ASC speck formation.. J Cell Biol 2017 doi:10.1083/jcb.201703103 PubMed 28701426
Sequence: download sequence
CTAGAGATTCTTGTTTAAGCTTTTAATCTCAAAAAACATTAAATGAAATGCATACAAGGTTTTATCCTGCTTTAGAACTGTTTGTATTTAATTATCAAACTATAAGACAGACAATCTAATGCCAGTACACGCTACTCAAAGTTGTAAAACCTCAGATTTAACTTCAGTAGAAGCTGATTCTCAAAATTGTTAGTGTCAAGCCTAGCTCTTTTGGGGCTGAAAAGCAATCCTGCAGTGCTGAAAAGCCTCTCACAGGCAGCCGATGCGGGAAGAGGTGTATTAGTCTTGATAGAGAGGCTGCAAATAGCAGGAAACGTGAGCAGAGACTCCCTGGTGTCTGAAACACAGGCCAGATGGGCCCTCGAGCAGGAAACAGCTATGACCATGATTACGCCAAGCTATCAACTTTGTATAGAAAAGTTGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATCCAGTCACTATGGTCGACCTCGAAACGTTCTAGAACTGGAGAACGTTCTAGAACGTCGAGAACGTTCTAGAACTGGAGAACGTTCTAGAACGTCGAGAACGTTCTAGAACTGGAGAACGTTCTAGAACGTCGAGAACGTTCTAGAACTGGAGAACGTTCTAGAACGTCGAGGGGGGGCCCGCGTGAGCTCGGTACGGGTCGAGGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCTGGTTTAGTGAACCCTCATTCTGCAGTAATACGACTCACTATAGGGCTAGCTTGATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGGATCAGCTTGCTTGTTCTTTTTGCAGAAGCTCAGAATAAACGCTCAACTTTGGCAGATCTATCGATAAGCTTGAATTCACCATGGCGGAATCTTTCAAGGAGCAGCTGCAGGAGGCTTTTGAGGATCTGGGTGCTGATAATCTTAGAAAGTTTAAAAGCAAACTGGGCGATCGGAGGCAGGAACCGCGCGTCACAAAGTCTGCAATCGAAAAGCTGAAAGACGAGATCGATCTGGCTGATTTAATGGTCGGCGTGTTCACATCAAAAGACGCGGTTTCTGTAACAGTGGAGATTTTGAGGGCGATCAAGTGTAATGCAGTAGCAGATGATCTATTGAGGAACACAGGGCAATCAGAAAGCAAAGGTGCTCCCTCTGATGAAAGCAAAGGTGCTTCCTCTAAGGCTGTAAGCAAAGTTGCTTTCTCCAAGGTAAACTTCATCGATGAGCACTGGAAAGAATTGATAGACCGAGTTAATAATGTGGACCCCATCCTAGATATACTTCGGCAGAAAAAAGTCATCACAAATGAGGATTTCTGTACCATTCGTAATAAGGAGACTCCTCAAAAGAAGATGAGAGAGTTATTAACAGGCCCAATCACATGTGCTGGCAACAAAGGGAAAGAAGTCTTGTATGATGCTCTCAGAGAGAGCAATAAATTCCTGATGGATGACCTTGAGGATGCTGAGGGTGGAGGCGGTATGGTGAGCGAGCTGATTAAGGAGAACATGCACATGAAGCTGTACATGGAGGGCACCGTGAACAACCACCACTTCAAGTGCACATCCGAGGGCGAAGGCAAGCCCTACGAGGGCACCCAGACCATGAGAATCAAGGCGGTCGAGGGCGGCCCTCTCCCCTTCGCCTTCGACATCCTGGCTACCAGCTTCATGTACGGCAGCAAAACCTTCATCAACCACACCCAGGGCATCCCCGACTTCTTTAAGCAGTCCTTCCCCGAGGGCTTCACATGGGAGAGAGTCACCACATACGAAGACGGGGGCGTGCTGACCGCTACCCAGGACACCAGCCTCCAGGACGGCTGCCTCATCTACAACGTCAAGATCAGAGGGGTGAACTTCCCATCCAACGGCCCTGTGATGCAGAAGAAAACACTCGGCTGGGAGGCCTCCACCGAGACCCTGTACCCCGCTGACGGCGGCCTGGAAGGCAGAGCCGACATGGCCCTGAAGCTCGTGGGCGGGGGCCACCTGATCTGCAACTTGAAGACCACATACAGATCCAAGAAACCCGCTAAGAACCTCAAGATGCCCGGCGTCTACTATGTGGACAGAAGACTGGAAAGAATCAAGGAGGCCGACAAAGAGACCTACGTCGAGCAGCACGAGGTGGCTGTGGCCAGATACTGCGACCTCCCTAGCAAACTGGGGCACAGATGAGATATCACTAGTGACTGACTAGGATCTGGTTACCACTAAACCAGCCTCAAGAACACCCGAATGGAGTCTCTAAGCTACATAATACCAACTTACACTTTACAAAATGTTGTCCCCCAAAATGTAGCCATTCGTATCTGCTCCTAATAAAAAGAAAGTTTCTTCACATTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCGTAGATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAATTCGCGGCCGCCACCGCGGTGGAGCTCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGCGCNNNNNNNNNNNNNNNNNNNNNNNNGGTACCATCGATGATGATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTATGGCTGATTATGATCCTCTAGATCAGATCTCTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGGATCTACGTATCAATTAAGTTTGTGCCCCAGTTTGCTAGGGAGGTCGCAGTATCTGGCCACAGCCACCTCGTGCTGCTCGACGTAGGTCTCTTTGTCGGCCTCCTTGATTCTTTCCAGTCTGTGGTCCACATAGTAGACGCCGGGCATCTTGAGGTTCTTAGCGGGTTTCTTGGATCTGTATGTGGTCTTGAAGTTGCAGATCAGGTGGCCCCCGCCCACGAGCTTCAGGGCCATGTCGCTTCTGCCTTCCAGGCCGCCGTCAGCGGGGTACAGCATCTCGGTGTTGGCCTCCCAGCCGAGTGTTTTCTTCTGCATCACAGGGCCGTTGGATGGGAAGTTCACCCCTCTGATCTTGACGTTGTAGATGAGGCAGCCGTCCTGGAGGCTGGTGTCCTGGGTAGCGGTCAGCACGCCCCCGTCTTCGTATGTGGTGACTCTCTCCCATGTGAAGCCCTCAGGGAAGGACTGCTTAAAGAAGTCGGGGATGCCCTGGGTGTGGTTGATGAAGGTTCTGCTGCCGTACATGAAGCTGGTAGCCAGGATGTCGAAGGCGAAGGGGAGAGGGCCGCCCTCGACCACCTTGATTCTCATGGTCTGGGTGCCCTCGTAGGGCTTGCCTTCGCCCTCGGATGTGCACTTGAAGTGGTGGTTGTTCACGGTGCCCTCCATGTACAGCTTCATGTGCATGTTCTCCTTAATCAGCTCTTCGCCCTTAGACACCATGGTCACTGTCTGCTTTGCTGTTGGTCTGGGCTCCTGGGTCACTGGCTTACTAATGGAGTCTTTATGTATGAGGACTCTTATCAATTGTTCTTCTATAAAGGTCTGCAGTGTTTCTGTTCGTCCCCTACATGGACACCCAGAGCCTCCTAAATACAGGAGCCCTGATAACTGCACAAGTGCTCAGATTCCAGCAGGGTGGAAAATGAGATAAAGTGTGCAGATGGGGAGGGGGACGTGAATGAGAGATTTGAGGGATGAAAAGGATGGATGAACGCATTGAAAATAGCCCCTTTCACAAAATAATACCAGTAAATTGTCATATAATTAATGACCAGACCTTTACCTGTAAATGATGTGATGTTCATATACAGAAGAATGCCAACAATTTACAGATGATTTTACAAATTCTTAATTTTCCTTTTCTAAATGGATTTGTTCACACATTATCTCTTCAAAGCAATTGAATTTTCTGGAAAAGATTGGCTGTGTGTGTAAAAGGTGCTACTGAGGAATTTAATGTGACATGGAATGAAGCAAACAGCAGCCTAAGACAGGATGGCAGGAAAAATGTCACATGTTTAAAATAAGAGTGAAACCAACGACCTGGACTTATAGAGACTGTGTGTCCTTAGGCAAAGCTCTTGTACAAACAGTGCTTGTGACTGTGAAATTCGGGGTTTGCCTGGATTGTGTTAAATGTGTGCTTGGATGTATCATAAAAAAGAATCGGTTCTCTGTATTTGACATTTTCTGTAATTTATCTAAAGTTACAACTGGCTATGCCTGATTTTTATCACTATTGAATCAATCATTTTAAAGAATGTTAAGTCTGCTGATTTGGAGAGCTCATTTACATTTATTTTGAATGTCTCTTATTTAACACAACTGATTTAAGCTTTAGATCTGCGAAGATACGGCCACGGGTGCTCTTGATCCTGTGGCTGATTTTGGACTGTGCTGCTCGCAGCTGCTGATGAATCACATACTTCCTCCATTTTCTTCCACTGATTGACTGTTATAATTTCCCTAATTTCCAGGTCAAGGTGCTGTGCATTGTGGTAATAGATGTGACATGACGTCACTTCCAAAGGACCAATGAACATGTCTGACCAATTTCATATAATGTGAAAACGATTTTCATAGGCAGAATAAATAACATTTAAATTAAACTGGGCATCAGCGCAATTCAATTGGTTTGGTAATAGCAAGGGAAAATAGAATGAAGTGATCTCCAAAAAATAAGTACTTTTTGACTGTAAATAAAATTGTAAGGAGTAAAAAGTACTTTTTTTTCTAAAAAAATGTAATTAAGTAAAAGTAAAAGTATTGATTTTTAATTGTACTCAAGTAAAGTAAAAATCCCCAAAAATAATACTTAAGTACAGTAATCAAGTAAAATTACTCAAGTACTTTACACCTCTGGTTCTTGACCCCCTACCTTCAGCAAGCCCAGCAGATCCACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGCGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACGCTGGCTAAGAACTCATCAGCCTCCCCGGTCCATCTACCCACGTACCAATGCACCAATTGGCCACAATGACGGCTACTACATGGTGCCATTCCTTCCTCTTTATAGGAATGGAGACTACCTCCTGTCCAACAAGGCTCTTGGATACGAGTACGCCTACCTGTTGGACCCAGGTCATTGCACAACACCAGAAATGCCCTCTGATCTGCAAAAGACGTGAATATCTGTTCAGACACCCATATCCACTCTGTTCCACACAGGTCAGAGGTTTGTCCAGGAGTTCTTGACAGAGGTGTAAAAAGTACTCAAAAATTTTACTCAAGTGAAAGTACAAGTACTTAGGGAAAATTTTACTCAATTAAAAGTAAAAGTATCTGGCTAGAATCTTACTTGAGTAAAAGTAAAAAAGTACTCCATTAAAATTGTACTTGAGTATTAAGGAAGTAAAAGTAAAAGCAAGAAAGAAAA
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