EPR Plasmid #421: Tol2(HSE:mCherrry-Cry2olig-zf_ASC_cmlc2:tagRFP)
Sequence: download sequence
| Purpose of plasmid | Expression of mCherry-Cry2olig-zf_Asc under heat shock promoter, contains Tol2 sites for genome integration and cmlc2:tagRFP for selection |
| Depositing Lab |
Maria Leptin European Molecular Biology Laboratory (EMBL) |
| Depositing Scientist | Eva Hasel de Carvalho |
| Antibiotic Resistance | Ampicillin |
| Bacterial Growth Temp | 37 |
| Copy Number | High |
| Selectable Marker | |
| Reference |
The Opto-inflammasome in zebrafish: a tool to study cell and tissue responses to speck formation and cell death Hasel de Carvalho, E.; Dharmadhikari, S.; Shkarina, K.; Xiong, J. R.; Reversade, B.; Broz, P.; Lept... DOI |
| MTA and Terms |
MTA (PDF download) |
How to cite this reagent:
In the Materials & Methods:
Tol2(HSE:mCherrry-Cry2olig-zf_ASC_cmlc2:tagRFP) was a gift from Maria Leptin (European Plasmid Repository plasmid #421).
In the References:
Hasel de Carvalho, E.; Dharmadhikari, S.; Shkarina, K.; Xiong, J. R.; Reversade, B.; Broz, P.; Lept.... The Opto-inflammasome in zebrafish: a tool to study cell and tissue responses to speck formation and cell death . doi: 10.1101/2022.10.19.512883
Tol2(HSE:mCherrry-Cry2olig-zf_ASC_cmlc2:tagRFP) was a gift from Maria Leptin (European Plasmid Repository plasmid #421).
In the References:
Hasel de Carvalho, E.; Dharmadhikari, S.; Shkarina, K.; Xiong, J. R.; Reversade, B.; Broz, P.; Lept.... The Opto-inflammasome in zebrafish: a tool to study cell and tissue responses to speck formation and cell death . doi: 10.1101/2022.10.19.512883
Sequence: download sequence
ATCACTAGTGACTGACTAGGATCTGGTTACCACTAAACCAGCCTCAAGAACACCCGAATGGAGTCTCTAAGCTACATAATACCAACTTACACTTTACAAAATGTTGTCCCCCAAAATGTAGCCATTCGTATCTGCTCCTAATAAAAAGAAAGTTTCTTCACATTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCGTAGATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAATTCGCGGCCGCCACCGCGGTGGAGCTCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGCGCCCCCCCCCCCCCCCCCCCCCCCCCGGTACCATCGATGATGATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTATGGCTGATTATGATCCTCTAGATCAGATCTCTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGGATCTACGTATCAATTAAGTTTGTGCCCCAGTTTGCTAGGGAGGTCGCAGTATCTGGCCACAGCCACCTCGTGCTGCTCGACGTAGGTCTCTTTGTCGGCCTCCTTGATTCTTTCCAGTCTGTGGTCCACATAGTAGACGCCGGGCATCTTGAGGTTCTTAGCGGGTTTCTTGGATCTGTATGTGGTCTTGAAGTTGCAGATCAGGTGGCCCCCGCCCACGAGCTTCAGGGCCATGTCGCTTCTGCCTTCCAGGCCGCCGTCAGCGGGGTACAGCATCTCGGTGTTGGCCTCCCAGCCGAGTGTTTTCTTCTGCATCACAGGGCCGTTGGATGGGAAGTTCACCCCTCTGATCTTGACGTTGTAGATGAGGCAGCCGTCCTGGAGGCTGGTGTCCTGGGTAGCGGTCAGCACGCCCCCGTCTTCGTATGTGGTGACTCTCTCCCATGTGAAGCCCTCAGGGAAGGACTGCTTAAAGAAGTCGGGGATGCCCTGGGTGTGGTTGATGAAGGTTCTGCTGCCGTACATGAAGCTGGTAGCCAGGATGTCGAAGGCGAAGGGGAGAGGGCCGCCCTCGACCACCTTGATTCTCATGGTCTGGGTGCCCTCGTAGGGCTTGCCTTCGCCCTCGGATGTGCACTTGAAGTGGTGGTTGTTCACGGTGCCCTCCATGTACAGCTTCATGTGCATGTTCTCCTTAATCAGCTCTTCGCCCTTAGACACCATGGTCACTGTCTGCTTTGCTGTTGGTCTGGGCTCCTGGGTCACTGGCTTACTAATGGAGTCTTTATGTATGAGGACTCTTATCAATTGTTCTTCTATAAAGGTCTGCAGTGTTTCTGTTCGTCCCCTACATGGACACCCAGAGCCTCCTAAATACAGGAGCCCTGATAACTGCACAAGTGCTCAGATTCCAGCAGGGTGGAAAATGAGATAAAGTGTGCAGATGGGGAGGGGGACGTGAATGAGAGATTTGAGGGATGAAAAGGATGGATGAACGCATTGAAAATAGCCCCTTTCACAAAATAATACCAGTAAATTGTCATATAATTAATGACCAGACCTTTACCTGTAAATGATGTGATGTTCATATACAGAAGAATGCCAACAATTTACAGATGATTTTACAAATTCTTAATTTTCCTTTTCTAAATGGATTTGTTCACACATTATCTCTTCAAAGCAATTGAATTTTCTGGAAAAGATTGGCTGTGTGTGTAAAAGGTGCTACTGAGGAATTTAATGTGACATGGAATGAAGCAAACAGCAGCCTAAGACAGGATGGCAGGAAAAATGTCACATGTTTAAAATAAGAGTGAAACCAACGACCTGGACTTATAGAGACTGTGTGTCCTTAGGCAAAGCTCTTGTACAAACAGTGCTTGTGACTGTGAAATTCGGGGTTTGCCTGGATTGTGTTAAATGTGTGCTTGGATGTATCATAAAAAAGAATCGGTTCTCTGTATTTGACATTTTCTGTAATTTATCTAAAGTTACAACTGGCTATGCCTGATTTTTATCACTATTGAATCAATCATTTTAAAGAATGTTAAGTCTGCTGATTTGGAGAGCTCATTTACATTTATTTTGAATGTCTCTTATTTAACACAACTGATTTAAGCTTTAGATCTGCGAAGATACGGCCACGGGTGCTCTTGATCCTGTGGCTGATTTTGGACTGTGCTGCTCGCAGCTGCTGATGAATCACATACTTCCTCCATTTTCTTCCACTGATTGACTGTTATAATTTCCCTAATTTCCAGGTCAAGGTGCTGTGCATTGTGGTAATAGATGTGACATGACGTCACTTCCAAAGGACCAATGAACATGTCTGACCAATTTCATATAATGTGAAAACGATTTTCATAGGCAGAATAAATAACATTTAAATTAAACTGGGCATCAGCGCAATTCAATTGGTTTGGTAATAGCAAGGGAAAATAGAATGAAGTGATCTCCAAAAAATAAGTACTTTTTGACTGTAAATAAAATTGTAAGGAGTAAAAAGTACTTTTTTTTCTAAAAAAATGTAATTAAGTAAAAGTAAAAGTATTGATTTTTAATTGTACTCAAGTAAAGTAAAAATCCCCAAAAATAATACTTAAGTACAGTAATCAAGTAAAATTACTCAAGTACTTTACACCTCTGGTTCTTGACCCCCTACCTTCAGCAAGCCCAGCAGATCCACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGCGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACGCTGGCTAAGAACTCATCAGCCTCCCCGGTCCATCTACCCACGTACCAATGCACCAATTGGCCACAATGACGGCTACTACATGGTGCCATTCCTTCCTCTTTATAGGAATGGAGACTACCTCCTGTCCAACAAGGCTCTTGGATACGAGTACGCCTACCTGTTGGACCCAGGTCATTGCACAACACCAGAAATGCCCTCTGATCTGCAAAAGACGTGAATATCTGTTCAGACACCCATATCCACTCTGTTCCACACAGGTCAGAGGTTTGTCCAGGAGTTCTTGACAGAGGTGTAAAAAGTACTCAAAAATTTTACTCAAGTGAAAGTACAAGTACTTAGGGAAAATTTTACTCAATTAAAAGTAAAAGTATCTGGCTAGAATCTTACTTGAGTAAAAGTAAAAAAGTACTCCATTAAAATTGTACTTGAGTATTAAGGAAGTAAAAGTAAAAGCAAGAAAGAAAACTAGAGATTCTTGTTTAAGCTTTTAATCTCAAAAAACATTAAATGAAATGCATACAAGGTTTTATCCTGCTTTAGAACTGTTTGTATTTAATTATCAAACTATAAGACAGACAATCTAATGCCAGTACACGCTACTCAAAGTTGTAAAACCTCAGATTTAACTTCAGTAGAAGCTGATTCTCAAAATTGTTAGTGTCAAGCCTAGCTCTTTTGGGGCTGAAAAGCAATCCTGCAGTGCTGAAAAGCCTCTCACAGGCAGCCGATGCGGGAAGAGGTGTATTAGTCTTGATAGAGAGGCTGCAAATAGCAGGAAACGTGAGCAGAGACTCCCTGGTGTCTGAAACACAGGCCAGATGGGCCCTCGAGCAGGAAACAGCTATGACCATGATTACGCCAAGCTATCAACTTTGTATAGAAAAGTTGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATCCAGTCACTATGGTCGACCTCGAAACGTTCTAGAACTGGAGAACGTTCTAGAACGTCGAGAACGTTCTAGAACTGGAGAACGTTCTAGAACGTCGAGAACGTTCTAGAACTGGAGAACGTTCTAGAACGTCGAGAACGTTCTAGAACTGGAGAACGTTCTAGAACGTCGAGGGGGGGCCCGCGTGAGCTCGGTACGGGTCGAGGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCTGGTTTAGTGAACCCTCATTCTGCAGTAATACGACTCACTATAGGGCTAGCTTGATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGGATCAGCTTGCTTGTTCTTTTTGCAGAAGCTCAGAATAAACGCTCAACTTTGGCAGATCTATCGATAAGCTTGAATTCGGATCCATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGCAGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGGGAGGAGGAGGAATGATGAAGATGGACAAAAAGACTATAGTTTGGTTTAGAAGAGACCTAAGGATTGAGGATAATCCTGCATTAGCAGCAGCTGCTCACGAAGGATCTGTTTTTCCTGTCTTCATTTGGTGTCCTGAAGAAGAAGGACAGTTTTATCCTGGAAGAGCTTCAAGATGGTGGATGAAACAATCACTTGCTCACTTATCTCAATCCTTGAAGGCTCTTGGATCTGACCTCACTTTAATCAAAACCCACAACACGATTTCAGCGATCTTGGATTGTATCCGCGTTACCGGTGCTACAAAAGTCGTCTTTAACCACCTCTATGATCCTGTTTCGTTAGTTCGGGACCATACCGTAAAGGAGAAGCTGGTGGAACGTGGGATCTCTGTGCAAAGCTACAATGGAGATCTATTGTATGAACCGTGGGAGATATACTGCGAAAAGGGCAAACCTTTTACGAGTTTCAATTCTTACTGGAAGAAATGCTTAGATATGTCGATTGAATCCGTTATGCTTCCTCCTCCTTGGCGGTTGATGCCAATAACTGCAGCGGCTGAAGCGATTTGGGCGTGTTCGATTGAAGAACTAGGGCTGGAGAATGAGGCCGAGAAACCGAGCAATGCGTTGTTAACTAGAGCTTGGTCTCCAGGATGGAGCAATGCTGATAAGTTACTAAATGAGTTCATCGAGAAGCAGTTGATAGATTATGCAAAGAACAGCAAGAAAGTTGTTGGGAATTCTACTTCACTACTTTCTCCGTATCTCCATTTCGGGGAAATAAGCGTCAGACACGTTTTCCAGTGTGCCCGGATGAAACAAATTATATGGGCAAGAGATAAGAACAGTGAAGGAGAAGAAAGTGCAGATCTTTTTCTTAGGGGAATCGGTTTAAGAGAGTATTCTCGGTATATATGTTTCAACTTCCCGTTTACTCACGAGCAATCGTTGTTGAGTCATCTTCGGTTTTTCCCTTGGGATGCTGATGTTGATAAGTTCAAGGCCTGGAGACAAGGCAGGACCGGTTATCCGTTGGTGGATGCCGGAATGAGAGAGCTTTGGGCTACCGGATGGATGCATAACAGAATAAGAGTGATTGTTTCAAGCTTTGCTGTGAAGTTTCTTCTCCTTCCATGGAAATGGGGAATGAAGTATTTCTGGGATACACTTTTGGATGCTGATTTGGAATGTGACATCCTTGGCTGGCAGTATATCTCTGGGAGTATCCCCGATGGCCACGAGCTTGATCGCTTGGACAATCCCGCGTTACAAGGCGCCAAATATGACCCAGAAGGTGAGTACATAAGGCAATGGCTTCCCGAGCTTGCGAGATTGCCAACTGAATGGATCCATCATCCATGGGACGCTCCTTTAACCGTACTCAAAGCTTCTGGTGTGGAACTCGGAACAAACTATGCGAAACCCATTGTAGACATCGACACAGCTCGTGAGCTACTAGCTAAAGCTATTTCAAGAACCCGTGGAGCACAGATCATGATCGGAGCAGCAGCCCGGGATCCACCGGTCGCCACCGGAGGAGGAGGAAGCGGAGGAGGAGGAAGCGGAGGAGGAGGAAGCGGAGGAGGAGGAATGGCGGAATCTTTCAAGGAGCAGCTGCAGGAGGCTTTTGAGGATCTGGGTGCTGATAATCTTAGAAAGTTTAAAAGCAAACTGGGCGATCGGAGGCAGGAACCGCGCGTCACAAAGTCTGCAATCGAAAAGCTGAAAGACGAGATCGATCTGGCTGATTTAATGGTCGGCGTGTTCACATCAAAAGACGCGGTTTCTGTAACAGTGGAGATTTTGAGGGCGATCAAGTGTAATGCAGTAGCAGATGATCTATTGAGGAACACAGGGCAATCAGAAAGCAAAGGTGCTCCCTCTGATGAAAGCAAAGGTGCTTCCTCTAAGGCTGTAAGCAAAGTTGCTTTCTCCAAGGTAAACTTCATCGATGAGCACTGGAAAGAATTGATAGACCGAGTTAATAATGTGGACCCCATCCTAGATATACTTCGGCAGAAAAAAGTCATCACAAATGAGGATTACTGTACCATTCGTAATAAGGAGACTCCTCAAAAGAAGATGAGAGAGTTATTAACAGGCCCAATCACATGTGCTGGCAACAAAGGGAAAGAAGTCTTGTATGATGCTCTCAGAGAGAGCAATAAATTCCTGATGGATGACCTTGAGGATGCTGAGTAAGAT
next plasmid