EPR Plasmid #420: ol_Il-1:GFP-T2A-Il-1-HA
Sequence: download sequence
| Purpose of plasmid | Il-1 Reporter: Medaka interleukin-1 fused N-terminally to GFP via T2A and tagged with an HA tag. Il-1 is expressed under its own promoter (ca. 2 kb). The plasmid has I-SceI sites for meganuclease insertion |
| Depositing Lab |
Maria Leptin European Molecular Biology Laboratory (EMBL) |
| Depositing Scientist | Eva Hasel de Carvalho |
| Antibiotic Resistance | Ampicillin |
| Bacterial Growth Temp | 37 |
| Copy Number | High |
| Selectable Marker | |
| Reference |
Revisiting the origin of interleukin 1 in anamniotes and sub-functionalization of interleukin 1 in amniotes. Hasel de Carvalho E et al. Open Biol 2022 PubMed DOI |
| MTA and Terms |
MTA (PDF download) |
How to cite this reagent:
In the Materials & Methods:
ol_Il-1:GFP-T2A-Il-1-HA was a gift from Maria Leptin (European Plasmid Repository plasmid #420).
In the References:
Hasel de Carvalho E et al.. Revisiting the origin of interleukin 1 in anamniotes and sub-functionalization of interleukin 1 in amniotes.. Open Biol 2022 doi:10.1098/rsob.220049 PubMed 35975650
ol_Il-1:GFP-T2A-Il-1-HA was a gift from Maria Leptin (European Plasmid Repository plasmid #420).
In the References:
Hasel de Carvalho E et al.. Revisiting the origin of interleukin 1 in anamniotes and sub-functionalization of interleukin 1 in amniotes.. Open Biol 2022 doi:10.1098/rsob.220049 PubMed 35975650
Sequence: download sequence
GTAAAACGACGGCCAGTGAGCGCGTAGGGATAACAGGGTAATGCGCGCGTAATACGACTCACTATANGGCGAATTGGGTACCGGGCCCCCCCTCGAGGAGGAGGAGTGGAAGTTCCAGAGCGGAGGAAGCAGAAATCATTTATGATCTGTAGTCAGCTGACTAATGTGTGTGTGGGGGAGGGGGGTCTTCTTTTACTACTTTAAACTCTGTCAGCAGTTTTTTCTTCTAGATTTGCTTTTCTATCTGCTTGTAGTTTTTCCTCATTTCTGAATGTGTTCTCCATCTTTGAATCTTCCTTTGTCCTAACAAAATTCTCTGAGGGCCTGAAAATAAAACCCAGTTGGAACTAAAAGCCACCATGATGACACTCCCACCGCCTGCACCGGAACAGGGAGCGACACGCTGAAAGGCCGGGAGCAGAGCCGGAAGTGAGCCGCTTTTGTCTCGGGTTCAATTCCAAAGGTGAGAAATTCCACAGCAGCCCCTCCCAGACGCACCGGGGACTTTTCTGGCTGAAACGGATCGGGGAGGGAATCAGGGCGTGTGCTGCTCAGAGGCCGATGCTCACCTGCACAGCTTTTCCTGACGGCTCCGTTCCAGGAATTCTGGCTGAGGGGGAAACTCTGCTCCGAGTCGGAGTTTCACCGGAAGAGTTACGCTGTGACGTGACCCTGAGGTCAAACCGCTGTGGGGGCCTCCATGATCCTGCTGAGACCAGATCTGGAAGAAGTGTGAACAGCCGGGCGTTAAATAAAGTTTATGAACCAAACTGCAAAAACTGGACTGAACAATAGATTTTTGTTGTTTATATAAGTCAAACAATTTTGTCTGCTGTTGTCTTATTTTGGTTTTCTACATTTGTTTTCACTTTTCCAAAATAGATTTCGACAAAAAGGACAACGCTGCAGACTTTTGTTTTCTTCTGAAATGTTCTTGATTATAAAGTTTTTTTCCTGGAAAATGATCCTTATTTCAGACTCCACCCGCCTTCATTACACTGCAAGGTTCATTTATTCAGTTCCAAACAGGAACGTTTCAAAGGTTCTGGTCACATCCTGGCTCCTTAGACATTTCTGAACAGATTCAAGGATCTTTAATTGTTGGGGTGAAACTAAACGAGTCCCTCTGGTTCTGGGCCGGCCAATCACAGAGCAAATAAAAATATGATTAAAAAAACGGAGCTAGATGCCATAAAAGAGTAGAAAAGCAACACTATAAATATAAACGGGTGTGTTGGTCCGTGGTTGTGGGTAAAGGGGGGTGAGGGGGTCAGCAGTCTTACAGCTTCCGGGTTGAAGCTGCTCCTTTAAGGTGGTGGTCCTGCTCTCTCTGGAGCATCGCTCTGATTGGTTAAAGTATTTGATCATTTTCACTGAGTCAGACGCGTCATTGCGCTTTTGCTGAGTCATGGAAATGGAAAAGTTTCTCATTTCTCTTCCTGTTTTGTGGCTTTACTAGGTTTGGATTTTTAGATGACCTCTTTGGGTTTTTTCTGAGATCAGATCCATTTTGCTGCCGTTACAATGAAGATTTATTTTGATTTCTACATTAAACTGATGAGGACCTGGACTTTGGTTCTGACTCGGTCTTTGGCCACTTTCACACTGGAATATTTGGTCCAGTTTGAAAGGGAATGCTGAGCTTTTGCCTCTTCCTGGTTGAAGAAAGTGTCTTTCTTCTCATGTTCTCGTCTTTGTAGAAGACTGTCTTTGTCTTTTTCCGGGACAGGAGTGTCTATTTGATCCTTTGTTCTTAGGCCTCTGCATGTTGGAGTCTAGAGCCGTGGCTTCAGAGGTTTTTATTTTCCTCCTCCAGGAGAATAGAGGAAGCTTTTGTCTCCTCGGCGTGGGCCTGCACGGACCTGCCTCGTCCAACCTGAATGTCCTGCAGCTGATCTCAACAGGATCATTTTGTCTTAGAAAAACTGGGGCGGAGATTTAGCGACGGATGTTTTTCATTCAGCTGTTGAAACCGTTTGTTCCTTTAAACACAGTTTACGAGGTTTCATCCTGGAAGCGTCCTTACAAAAAAAACAGGGACTTCTACAAGAAAAAGTCCAAGAGCAAAATCCAGATGACTTGTCCATTCGTGCAGGAAGTGAAGTACTGAACCATTAATGAATGTTTTCTGAAGATAATCATTTTGTTATTAAAAAGAATCTATCGGATCAGAACCGGATACAAAAACTTTAAAACTGTAATATGAATTAGGGGGTGAGATTATATAAATTCACTTCTTCCCGCTCCTTTTCAAGCAATAAATCAAACTTTACTAGATTTTAGTTACTGATCACTGTTTTTAATTAAGCAAAATGTGATTTAAGTGACTTTTGTTTGTTAAACTATGCATTTCAACATTTCTGTAATGAGTTTGATAAATATGTGTAAATTCTTTATTGCTTTGACTACAATGCTTGAGATAAATCAATAAATCAAATCAATCAATCAATCACTGCGCAGCACAGACAGAGTGGCGTGTAAATTCAGTCGCGTCTCTTCGTCACAGGACTTCCTCCTCCGCCCTGAAGCACGATGCAGATGCGAAAGTGTCCCTCCGGCGCTCAAATCAGCTGCAGATTGAAGCCGACAGGAAGTGCGGTATTAGTCAGGAGCAATTAAAACCTCAGGAATTCATGCAAACGCCATGAGGCTCAAACACGCCCTGGTCATGGAGGTCAAACACGATCAGACGGTGGAAGTCTGGGTGTGAAACACGTTTATGAGAGAAAACAGGGCAGCTGTGACAAAGAAGAAGAAAAAACAGAAAATAAGCAGGACTGCTGTCCAGCAGGCCTTTGAGGATGCACAGGTACAAACTCGCACAGGTGTGGTGGGAAATTCCTGAGTGGCAGTTCTGTGTTTGACCCAGATTAAAGGAAACAGTTTTCTAAAATCCTCTCATCAAACGTCTGACTGAAGCTGTGAAAGGAAAGAAAAGCTTTAAAACCTCAGGAACACCCCCCCCCCCCCCCCCCCCAAAAACCAANNNNNNNNNNCTCACAAAAACCAGAAGTTTATTTAGTAAAGTACACTTGAGTATAAGTATGGCAAGTATATACAGACCAAGTAAACAAACTGAATACTTCTTCAGACTGGAACGTTTCAGTTTGACTGCTTTCTGCTGCCTTCATATCGTGACTTTATTGTTGTTTCCATCATTCTGAACCTCACGGAGGAACAAGCTGAAACCAGAAAAACATGACAAAAGTGACACTTTTACTTCTTTTTTTTGGTTTTTCTCTCTTTCAAAATACAAATGTGCAGTTTTTGTCTTCCTGTTTCCCACAATTCAGAAGAATAAATCTGAAACCAAATGAACCTGAGACTTGTTTGTCCTTTTTTTTGGATGAAAACGTTTAACTTTTGCGTCTTTTGTTGTGTTTCTGTTTGCAGTCATGAAGCCAGTCGCCCCGATGTGTGAATGTTTGTTTTATGGTCCTATCAGTGCTTCATTTGGATCTTCTGAGGATCAACAAGTGAAAAGGTTGTTGATCTGAATCTTTCTAAAATGTCGACTTCTCCAGCTTGTGGTCGACTGGAGCAGCTGAAGATCCGGTTCATGGAACTGAAAGTTAAAGTTGGACATTTTGAACTCAAAATAAATCAAAGGGTTAACTCTGTATTTTCCGTGTTGTTCCCCCTCCCTGTTGTTGACTCAGATGAGCATCTCTTGTGTGATCATCGCTCTGAGGACTTTCATCAGTGACGTGCTTTGACTTCCTGAACAGATCCAGCCGTTTGGACCAGACCGGGTCTCTGCTGCTGACGCAGTTCTCCTTCGTCGCACACTAACGAGGATGTGAGGAAATCTGAGATTCAGCCGCTGTTGTGCAACAAACAAGCTTTTGAAGACTTGTGAAAACTTTTCTCACAACAAAAGAACCACAAAACTCCATAGGGTTAACCAAAGAGATGAGAAAAGTTTGGAAATAACAGAAGAAAAAGTCTCATTTCTTTAGGTGAGCATTTCCAAATTCACTGCAAGACGTGTTTTCAGATGATCTGGAGTGTTTTTTTTTTTACACGCAATAGTAATGGAAAGGGTTTCTTTCAATCGATCAGAACAAAAAACATCTTTTTGATCAACACAGAAACTAATTTAAAGAATCCTTCGGCTTTTCATCAGTAAAATAACTGCAATGACAGATTTACTCAAAGTCTTGATGCTGAGCTTTAGTTCTACAGCTGGATCAACAAATATCCCTAAAAAAACATTTGACAAACTTTTATTTCCTTTAGACCAAATGGATCATCTCACTAAATGTGAATATATGTTAAAAAAAAAATTTTTTATTGACGACAAGAGATGGAAAAAAAAGTTGTCCACTACAATTCCCAGAATCCCGTACTTTAACATTTGGAGTCTTGAAAAGCCCCAAATGTCCTCTGCAGAAACCAAAAGGAGTCCATTCAATAATCTGCGGTGGTTGGAGATGTTCAGGTGTGGAAATGTCCTCTACAGGGGACACATTTGTGGTAGGCAGGAGGTAAAAGAACTTTAAGACGTGCCCCAGGATACCAATCAAATCCAGAATGCTCTTTTTTTATCTTTAGAAAGAGTTTGCTATTAGCTCCATACATTTTTCTGCACTGGAAACCCTCCATCCCGTTGTTCCATTTGTGTCTCTCAAATATTCTGGAATAAGTGCTGCTGGAGGCACCCACGAACGATTGCATCGATCAGGTCATATGGCGTGCAAACACCTGCACCACAGACCATGCAGAAAGGTTTTCATTAGCATGAGCTGCAAAAGGCAAGAAAGTGTTCATGCAAATGTCAAATGTGATCCAAAATAAAGGCTGTTGCAGAATCCTGTTTTTGACTTTCCTTTTTCTAGATTCAAACCTTTTTCTCCCTTTACTGTTAAAGTTCCAATAATGGGATGACATCCCCAATCAAACTCTGAAATACCACTAAATAAAGTCTTGAAAGATTGAACAGTTTAAATTCTCCTGATCACCAGAGAAATAGCGCGGTGGTGGCAGCATCATGCTGTGTGTGTAGTTTGTTCATATTTAAACGAGTTTATGCATGTTGACAGTCTCCTGAAGTTTTGGGTCCCCCTTCACCGATACCTGACGACGCCTTGGTGTCGTTTAGGCAACATTTCCACCAGAAGATTGGTTTCTTCCTTAAAGTCCCACTCCGATCATCTTCTGATCTCTTCCCAAACCGGTCCCAGTCGTCTTTTCACGATGATGATGCCGCTGTTTTTAGCCAAAATTAAAGAAAAAAGGTGTTTTTTAGGACATAGTTTCTGCAGAGCGGCAGGAGTTTATCAGAAATTCACCTCTGAGTTGTGAACAGGACTGTTCCGCCCCTCTTTCCATCACCTATCTGCATAAACTTTCACCCACTAGCTTACAGCCCCTCAGAACCCCAAGCTAGCATTAGCGGTGCAGCAAAAATGGCTGCAATATCAGATCCATCCATCCGTCCGTCCGTCCAGTTCTGATCCAGATTCCAGCTCAGACGAGGAAAACAAAGACGATCATGGATCTGTTGGTCTGCAGGTGGAGGATCAGAAAGGGCGGAGCAGGGAGACTTTAGCCCCGCCCAGAGTATTTCCTACGATCTTTTTCAAACTGCATTATTTTCGTCTGCTCCTGGTTCACAGCGATTTGATTTGATTTGATTGATCTTTTTCAAGCATTGTAGTCAAAGCAATAAAGAATATACACATATTTATCAAACTCATTACAGAAATGATGAAATGCATAGATTAAAAAGAAAGAAAGAAAAAAACAAAACAAAAACATCACTTAAATCACTTTGCTTAATTAAATACAGCCATAATTAACTAAAGTAAAGTAGAGTTTGATTTATTGCTTGAAAAGGAGCGGGAAGAAGTGAGATTATATAATCCCCCCCCCCGACTGCATTCATTCATTTGATAGTTTTACAGTGTTTTAAATCCGGTTCTGATCCGATTCTCTTCAAGTAACAAAATCCGGTGGCTATTAATGATTGATGATCTTCAGAAAACATTCTTTCATGATTTCAGTAAACGGTAACCATTATGTAATGATCATTGATTATCATTAGAACACATGATCACAATAAACCAGTAATTATTGCTACACATTGCAGATCAAGAAAAGAGAATCAATAGTTATTGGTTGTAATTCAAACCTTTCAGTAATCATTAATGCACATTACACATTAATACTCATTGGTTGTAATTACAACAGCTTTGATTATTTCACCACAATCAAGTTCACACATGGATTTGAATTATTCAAACACCTGTTGATCCTTAAATCTTCTTATCTTCATATCCTGAAATCAGAGTTTCTTTCTACATTTTCTTGAATATTTGAATATTGTTTGAGCTCATTACTTAGACCGTTTAAAGAAGAAATACTCAGACATGCAGATTTTAAGCTGAATTTTCTTCATACATGTCCTCCGTCATCAGAAAAATGCTACAAGAATGTGTTAGAAACCCCAAAAACACTGTTTCCAACAGAGTGGAGCTTTAGGGAGGACTGGAGGACGTATCCAGGACATAATTCCAGGAAGGTCTTTTTTAGTTCAAACATCGTAAAAGATTGTTTTTCCAGGTTAAAGGAGAAATGTGACCAACGGTAAATTATAATCAACCATAAAAGAGTCATAACAGAGTTCACTGCTCTTTTAAAGGGTCTGAATTTTCAGTTTCTCTGCTTTGTTGACAGTGAAACCTTCCTCTCCTGACAGTTTATTGGGAATCATTTGTGATGATTCTCTGTTAACGTCTGTCCCAAAGAACTGACTGAAAGTGGTTCAAAGCCGGATCTTCTAATCCATGAAACCTGTCTGCAGTAAAAAAGCGTGAGTGTGAAGCTGCCTGCAGTGAAGCAACATGTGCAATTACAGCATTTCATCATCGCAGCGAGAGCGGCTGGTAGCCCGTCCCCGTCTGCGGATATAAAAGGCTGCAGGTCAGCACAGGCAGGCAGAACCGCAGCACAGCTCACAGCAAGCACACAGCAACACAGGATCTGACAGGTAACGGGACCTCTCTGCCTTCATCTGCAGGTTTGAGTCCGCAAAGAAGGTTGCTGTTGAAACTAAGAATGTCTCTGTTTGCCGGCACCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGGAGGGCAGAGGAAGTCTTCTAACATGCGGTGACGTGGAGGAGAATCCCGGCCCTATGGAATCTGAGATGGCGTGCCACAAGAGCGACACGTACAGAATGAAGATGTCCCACGGCCTGCATTTGGAGATCTCGCATCATCCTCACTCGATGAGGAGCGTTGTCAATCTCATCATTGCCATTGACCGCCTGAAGGCCGGCGGGATGGAGGAGGTGATGGGTACTGACTTCAGGGACGAGCACCTTCTCAGCGTCATGCTGGAGAGCATTTTGGAAGAGCGACATGTTGCTGAGAGGTCTTCTGCTCCCAGCCAGTTCAGCAGAACCGGGGAGTATCAGTGCAGCGTGACGGACAAGCAGAAGAGGAGCTTGGTTCTGGTCAAGAACAGCATGAAGCTGCTCGCCGTGATGCTGCAGGGAGGCAGCGACAGCCGCAAAGTCCAGCTGAACATGTCTACTTACCTTCACCGGGACCGCACGCCTGAGGCCAGGCCGGTGGCTCTGGGCATCAAGGACACCAAACTCTACCTGTCCTGCCACCAAGAAGGAGACAAACCAACTCTGTTTCTGGAGGAGGTGGAGGACAACAGCACCCTTTCCACAATTGGCCAGGAGAGCGAGAATGTGCGCTTCCTCTTCTACAAACAGGACACGGGTGGAAACCTCAGCACCCTGGCGTCCGCCCGCTTCCCCGGCTGGTACATCAGCACGGCGGCGATCGACAGTGAACCCGTGGAGATGTGCCAGGAGAGCGACAGCCGCTTCAGGACCTTCCACATCCGCCAGAGCGGCGGTTACCCATACGATGTTCCAGATTACGCTTAAACTAGTGACTGACTAGGATCTGGTTACCACTAAACCAGCCTCAAGAACACCCGAATGGAGTCTCTAAGCTACATAATACCNACTTACACTTTACAAAATGTTGTCCCCCAAAATGTAG
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