EPR Plasmid #353: pRSFDuet-1 6xHis-HRV3C-Naa35 | Naa38ΔC11 | Naa30ΔC17
Sequence: download sequence
Purpose of plasmid | Coexpression of the three NatC subunits from S. cerevisiae in E. coli for affinity purification via a cleavable His-tag on Naa35 |
Depositing Lab |
Oliver Daumke Max-Delbrueck-Center for Molecular Medicine |
Depositing Scientist | Oliver Daumke |
Antibiotic Resistance | Kanamycin |
Bacterial Growth Temp | 37 |
Copy Number | High |
Selectable Marker | |
Reference |
Divergent architecture of the heterotrimeric NatC complex explains N-terminal acetylation of cognate substrates. Grunwald S et al. Nat Commun 2020 PubMed DOI |
MTA and Terms |
MTA (PDF download) |
How to cite this reagent:
In the Materials & Methods:
pRSFDuet-1 6xHis-HRV3C-Naa35 | Naa38ΔC11 | Naa30ΔC17 was a gift from Oliver Daumke (European Plasmid Repository plasmid #353).
In the References:
Grunwald S et al.. Divergent architecture of the heterotrimeric NatC complex explains N-terminal acetylation of cognate substrates.. Nat Commun 2020 doi:10.1038/s41467-020-19321-8 PubMed 33139728
pRSFDuet-1 6xHis-HRV3C-Naa35 | Naa38ΔC11 | Naa30ΔC17 was a gift from Oliver Daumke (European Plasmid Repository plasmid #353).
In the References:
Grunwald S et al.. Divergent architecture of the heterotrimeric NatC complex explains N-terminal acetylation of cognate substrates.. Nat Commun 2020 doi:10.1038/s41467-020-19321-8 PubMed 33139728
Sequence: download sequence
GGGGAATTGTGAGCGGATAACAATTCCCCTGTAGAAATAATTTTGTTTAACTTTAATAAGGAGATATACCATGGGCAGCA
GCCATCACCATCATCACCACAGCCAGGATCCGCTGGAAGTTCTGTTCCAGGGGCCCATGGAAGTAGACAGTATATTAGGT
TCCTTGAGCATAACTGATGATTTTGACCAATTGGTTGACGTCACAAGTCTGTTTGATGAATTGTGTTCAAAACTAAAACC
AGAGGCTATCGTGAAAGATCCCAGGTTTGATTTGTTCGAGGGTACACATTCTTTAGAGGTCAACAACTCCAAATTAGACT
CCAGTCTGATAGAATTGACAGCAGAAGAAATAGAATTTGATGTGAACGTGGCCTACGATCCACCGTTGGCAAGTGTAGCA
GCCATAGCGGATAGATTACTTAGATGTGTTATCTCCTGGCTGAATGACTACCAGACTTTACCCACTACTGTATTAAGCTG
CAGGTATACAGAAAGCTTATTATCGTCATTAGTTAAGGGAACAACTGCCGGTTCGTCCTGGTGTACAGGGAATATTTTGT
ATGATAAAGTACTAGGAAGTTGCATACTTGGCGTTTGTTATTTAACAAAATTTGTGCAGAAACTCTTGAGTGCTGGTATT
GTTTTTGAAGAGGAGGATTTAAATTTTAATAACATGGGGTTTAATACATTCGATAATTTGCCGGGGCAAGATGTTGTTAT
CAATTCTCTCACCGAGAGCCTTCAGATATTAGAGGCATATTCTGATGATAGCTTACATTTAACTATGCTGAAGCATATAC
TAAAAATTATCATTTGCTTAGTCCACTTAGAAGACCATCTGACAGACTATTCCACAAAGACAAGCCACCTGGATGAGCTA
ATTGAAAACGCTAACTCGGTTAATGGCATATTTCCACAATTACAGCTGTCTCCACCAAAGGGTGCGTTCTCAACATATAT
ACAAAAGCACAGGTCTAATCAATTCCCTCCCAGGAAGATTACGAAGTTACCGACGGATTACAGCGGCTTTATTACACTGG
CAAATGATGTTAAAACAATACTCCTTGTAGATAAAGCCGAGTCTGCGTTGGAGACCTACCAGTTTGCCAAATTCTTCAAC
AAGTTGGAACAAAGGCATGTCATTGCAAGGATCTTATTCCCCTTATTTTTCATTAGAGACGACAGAACAGTTTTGGGTAA
ATTTTCATATACCCAATTTTATTTGCTACATGTCAAGGAGTTTTCAGCACAAACTCCATCTGAATTTGAATCCTCTATTG
GTAATGAACTTATCCAAGAAAGCTCTAATATGCTTTTAGAATGGTACCAAAACTGCTCCCAAAACACATGTAGATATAGA
CAAGGTTTTAACAGGCAACTTATTCTATGGGATTCTCTTCAAGCCCAGTTTGAAAGTGTCAATTCACAAGTTTACTGTTC
GTGGACATATTTTATGAAACTATCAAGTATGATCGAATTCTCTTTAAAGGGGTTTGATTTGGATATTTACAAACCCTTTG
AAGCATACTCTATGTTTTGGTATGTTTATTATTTAAGTCACCACTTAGAAACCTTTTTGAAAGATAGTCAAAATGATATT
GAATCAAATATTAATGCCATTCATTCCATGAACAAAAAACTTAAAAAATTGAAAGCTGGTGAAAAGAAGGACCAATTGAG
GCTAAAGTACAGGTTTGCTATGGATAATGAAATGGAACAATTACAAGCTACCAAACAATTTTTAAACTATCTTTTGAAGG
AAATTAACATTACCAAATCTTTATGTCTAATAGAGGTTTTCCAATTTGCAATTTTAAAGTCATTTGGTTTAATTGACAAC
AAGAATAGTACTCCGTCAAAATTCTCTAATGAACGGTTAATCCATAATTTGAGATTCAAGCCATTTAATTCAATTGGTGT
TCCTGAGTTACCAGAATATGAAGTATTTCAACAAACTTTAAAAGATTTTGTCATTGAAGAAAAAGGAGCTGCATTTGATA
TAAAGTTAGAAAGGGCCACTAACTTTATCGAAACTGAAGTGCGCAATGTCGTTAGTTCTATTGATGAGATAATGCAAGGA
ATCAAGGGAGGCGATAATAACGGCGTTCTAGTAACAGGAACAAGACTGGTTCAAGAGCTCTCTCTGGAGTACTATTGTAA
ACTAAAACATACTTCTAAAGCATTATCAGTCAACTCAAAAGTTATCGTCAACACTTTGAAAAAGAACATTAAAAACAAGG
ACTCCCACGAATACAAGGTTGAATTAGTTCATACAACAGAAGGATGGAACTATTTCCCCATACAAACATTGAGAATAAAG
CAAGACCGCTATAAATAAGCGGCCGCATAATGCTTAAGTCGAACAGAAAGTAATCGTATTGTACACGGCCGCATAATCGA
AATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCATCTTAGTATATTAGTTAAGTATAAGAAGGA
GATATACATATGGACATCTTGAAACTGTCAGATTTTATTGGAAATACTTTAATAGTTTCCCTTACAGAAGATCGTATTTT
AGTTGGAAGCTTGGTTGCTGTAGATGCCCAAATGAATTTGCTATTAGATCATGTTGAGGAACGTATGGGCTCCAGTAGTA
GAATGATGGGCCTAGTCAGCGTCCCTAGGCGTTCCGTTAAGACCATAATGATTGATAAGCCTGTTCTGCAGGAGCTTACT
TAGCAATTGGATATCGGCCGGCCACGCGATCGAAGGAGATATACCATGGAAATAGTGTACAAGCCATTGGACATACGTAA
CGAAGAGCAGTTTGCTAGTATTAAAAAACTAATAGATGCGGATCTATCAGAGCCGTACTCGATATACGTATATAGGTATT
TCTTGAACCAATGGCCCGAGTTAACGTACATTGCCGTTGACAACAAGAGTGGAACGCCTAACATACCCATTGGTTGCATA
GTGTGTAAGATGGACCCGCATAGAAACGTGAGACTGAGAGGATATATAGGAATGCTAGCCGTAGAGTCCACATACCGCGG
GCATGGCATAGCAAAGAAACTAGTGGAAATTGCCATAGATAAAATGCAAAGGGAACATTGCGATGAGATCATGTTAGAAA
CAGAGGTGGAAAATTCGGCGGCTCTAAACTTATATGAAGGAATGGGTTTCATCAGAATGAAACGGATGTTCCGCTACTAC
TTGAATGAAGGGGACGCCTTTAAACTGATATTGCCGTTGACTTAATGACTCGAGTCTGGTAAAGAAACCGCTGCTGCGAA
ATTTGAACGCCAGCACATGGACTCGTCTACTAGCGCAGCTTAATTAACCTAGGCTGCTGCCACCGCTGAGCAATAACTAG
CATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAACCTCAGGCATTTGAGAAGCACACGGTCACA
CTGCTTCCGGTAGTCAATAAACCGGTAAACCAGCAATAGACATAAGCGGCTATTTAACGACCCTGCCCTGAACCGACGAC
AAGCTGACGACCGGGTCTCCGCAAGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATA
CATTCAAATATGTATCCGCTCATGAATTAATTCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATAT
CAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATG
GCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAG
GTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGTTTATGCATTTCTTTCCAGACTT
GTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGA
GCGAGACGAAATACGCGGTCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAG
CGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGA
GTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTG
ACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATA
CAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGG
AATTTAATCGCGGCCTAGAGCAAGACGTTTCCCGTTGAATATGGCTCATACTCTTCCTTTTTCAATATTATTGAAGCATT
TATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGCATGCAGCGCTCTTC
CGCTTCCTCGCTCACTGACTCGCTACGCTCGGTCGTTCGACTGCGGCGAGCGGTGTCAGCTCACTCAAAAGCGGTAATAC
GGTTATCCACAGAATCAGGGGATAAAGCCGGAAAGAACATGTGAGCAAAAAGCAAAGCACCGGAAGAAGCCAACGCCGCA
GGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGCCAGAGGTGGCGAAACCCGACAG
GACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATAC
CTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTTGGTATCTCAGTTCGGTGTAGGTCGT
TCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGT
CCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCATTGGTAACTGATTTAGAGGACTTTGTCTTGAAGTTATG
CACCTGTTAAGGCTAAACTGAAAGAACAGATTTTGGTGAGTGCGGTCCTCCAACCCACTTACCTTGGTTCAAAGAGTTGG
TAGCTCAGCGAACCTTGAGAAAACCACCGTTGGTAGCGGTGGTTTTTCTTTATTTATGAGATGATGAATCAATCGGTCTA
TCAAGTCAACGAACAGCTATTCCGTTACTCTAGATTTCAGTGCAATTTATCTCTTCAAATGTAGCACCTGAAGTCAGCCC
CATACGATATAAGTTGTAATTCTCATGTTAGTCATGCCCCGCGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCTCAA
GGGCATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGT
CGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGT
GGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGT
CCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTA
TCGTCGTATCCCACTACCGAGATGTCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCAT
CTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGG
CACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCCAGCCAGACGCAGA
CGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCGCGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAG
TCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACAT
TAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCG
AGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTG
ATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCA
ACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCC
CGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGAC
ATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGG
TTTTGCGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCCCTTATGCGACTCCTGCATTAGGAAATTAATACGACTCAC
TATA
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