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EPR Plasmid #353: pRSFDuet-1 6xHis-HRV3C-Naa35 | Naa38ΔC11 | Naa30ΔC17
Purpose of plasmid Coexpression of the three NatC subunits from S. cerevisiae in E. coli for affinity purification via a cleavable His-tag on Naa35
Depositing Lab Oliver Daumke
Max-Delbrueck-Center for Molecular Medicine
Depositing Scientist Oliver Daumke
Antibiotic Resistance Kanamycin
Bacterial Growth Temp 37
Copy Number High
Selectable Marker
Reference Divergent architecture of the heterotrimeric NatC complex explains N-terminal acetylation of cognate substrates.
Grunwald S et al. Nat Commun 2020
PubMed DOI
MTA and Terms MTA (PDF download)

How to cite this reagent:
In the Materials & Methods:
pRSFDuet-1 6xHis-HRV3C-Naa35 | Naa38ΔC11 | Naa30ΔC17 was a gift from Oliver Daumke (European Plasmid Repository plasmid #353).

In the References:
Grunwald S et al.. Divergent architecture of the heterotrimeric NatC complex explains N-terminal acetylation of cognate substrates.. Nat Commun 2020 doi:10.1038/s41467-020-19321-8 PubMed 33139728


Sequence: download sequence
GGGGAATTGTGAGCGGATAACAATTCCCCTGTAGAAATAATTTTGTTTAACTTTAATAAGGAGATATACCATGGGCAGCA GCCATCACCATCATCACCACAGCCAGGATCCGCTGGAAGTTCTGTTCCAGGGGCCCATGGAAGTAGACAGTATATTAGGT TCCTTGAGCATAACTGATGATTTTGACCAATTGGTTGACGTCACAAGTCTGTTTGATGAATTGTGTTCAAAACTAAAACC AGAGGCTATCGTGAAAGATCCCAGGTTTGATTTGTTCGAGGGTACACATTCTTTAGAGGTCAACAACTCCAAATTAGACT CCAGTCTGATAGAATTGACAGCAGAAGAAATAGAATTTGATGTGAACGTGGCCTACGATCCACCGTTGGCAAGTGTAGCA GCCATAGCGGATAGATTACTTAGATGTGTTATCTCCTGGCTGAATGACTACCAGACTTTACCCACTACTGTATTAAGCTG CAGGTATACAGAAAGCTTATTATCGTCATTAGTTAAGGGAACAACTGCCGGTTCGTCCTGGTGTACAGGGAATATTTTGT ATGATAAAGTACTAGGAAGTTGCATACTTGGCGTTTGTTATTTAACAAAATTTGTGCAGAAACTCTTGAGTGCTGGTATT GTTTTTGAAGAGGAGGATTTAAATTTTAATAACATGGGGTTTAATACATTCGATAATTTGCCGGGGCAAGATGTTGTTAT CAATTCTCTCACCGAGAGCCTTCAGATATTAGAGGCATATTCTGATGATAGCTTACATTTAACTATGCTGAAGCATATAC TAAAAATTATCATTTGCTTAGTCCACTTAGAAGACCATCTGACAGACTATTCCACAAAGACAAGCCACCTGGATGAGCTA ATTGAAAACGCTAACTCGGTTAATGGCATATTTCCACAATTACAGCTGTCTCCACCAAAGGGTGCGTTCTCAACATATAT ACAAAAGCACAGGTCTAATCAATTCCCTCCCAGGAAGATTACGAAGTTACCGACGGATTACAGCGGCTTTATTACACTGG CAAATGATGTTAAAACAATACTCCTTGTAGATAAAGCCGAGTCTGCGTTGGAGACCTACCAGTTTGCCAAATTCTTCAAC AAGTTGGAACAAAGGCATGTCATTGCAAGGATCTTATTCCCCTTATTTTTCATTAGAGACGACAGAACAGTTTTGGGTAA ATTTTCATATACCCAATTTTATTTGCTACATGTCAAGGAGTTTTCAGCACAAACTCCATCTGAATTTGAATCCTCTATTG GTAATGAACTTATCCAAGAAAGCTCTAATATGCTTTTAGAATGGTACCAAAACTGCTCCCAAAACACATGTAGATATAGA CAAGGTTTTAACAGGCAACTTATTCTATGGGATTCTCTTCAAGCCCAGTTTGAAAGTGTCAATTCACAAGTTTACTGTTC GTGGACATATTTTATGAAACTATCAAGTATGATCGAATTCTCTTTAAAGGGGTTTGATTTGGATATTTACAAACCCTTTG AAGCATACTCTATGTTTTGGTATGTTTATTATTTAAGTCACCACTTAGAAACCTTTTTGAAAGATAGTCAAAATGATATT GAATCAAATATTAATGCCATTCATTCCATGAACAAAAAACTTAAAAAATTGAAAGCTGGTGAAAAGAAGGACCAATTGAG GCTAAAGTACAGGTTTGCTATGGATAATGAAATGGAACAATTACAAGCTACCAAACAATTTTTAAACTATCTTTTGAAGG AAATTAACATTACCAAATCTTTATGTCTAATAGAGGTTTTCCAATTTGCAATTTTAAAGTCATTTGGTTTAATTGACAAC AAGAATAGTACTCCGTCAAAATTCTCTAATGAACGGTTAATCCATAATTTGAGATTCAAGCCATTTAATTCAATTGGTGT TCCTGAGTTACCAGAATATGAAGTATTTCAACAAACTTTAAAAGATTTTGTCATTGAAGAAAAAGGAGCTGCATTTGATA TAAAGTTAGAAAGGGCCACTAACTTTATCGAAACTGAAGTGCGCAATGTCGTTAGTTCTATTGATGAGATAATGCAAGGA ATCAAGGGAGGCGATAATAACGGCGTTCTAGTAACAGGAACAAGACTGGTTCAAGAGCTCTCTCTGGAGTACTATTGTAA ACTAAAACATACTTCTAAAGCATTATCAGTCAACTCAAAAGTTATCGTCAACACTTTGAAAAAGAACATTAAAAACAAGG ACTCCCACGAATACAAGGTTGAATTAGTTCATACAACAGAAGGATGGAACTATTTCCCCATACAAACATTGAGAATAAAG CAAGACCGCTATAAATAAGCGGCCGCATAATGCTTAAGTCGAACAGAAAGTAATCGTATTGTACACGGCCGCATAATCGA AATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCATCTTAGTATATTAGTTAAGTATAAGAAGGA GATATACATATGGACATCTTGAAACTGTCAGATTTTATTGGAAATACTTTAATAGTTTCCCTTACAGAAGATCGTATTTT AGTTGGAAGCTTGGTTGCTGTAGATGCCCAAATGAATTTGCTATTAGATCATGTTGAGGAACGTATGGGCTCCAGTAGTA GAATGATGGGCCTAGTCAGCGTCCCTAGGCGTTCCGTTAAGACCATAATGATTGATAAGCCTGTTCTGCAGGAGCTTACT TAGCAATTGGATATCGGCCGGCCACGCGATCGAAGGAGATATACCATGGAAATAGTGTACAAGCCATTGGACATACGTAA CGAAGAGCAGTTTGCTAGTATTAAAAAACTAATAGATGCGGATCTATCAGAGCCGTACTCGATATACGTATATAGGTATT TCTTGAACCAATGGCCCGAGTTAACGTACATTGCCGTTGACAACAAGAGTGGAACGCCTAACATACCCATTGGTTGCATA GTGTGTAAGATGGACCCGCATAGAAACGTGAGACTGAGAGGATATATAGGAATGCTAGCCGTAGAGTCCACATACCGCGG GCATGGCATAGCAAAGAAACTAGTGGAAATTGCCATAGATAAAATGCAAAGGGAACATTGCGATGAGATCATGTTAGAAA CAGAGGTGGAAAATTCGGCGGCTCTAAACTTATATGAAGGAATGGGTTTCATCAGAATGAAACGGATGTTCCGCTACTAC TTGAATGAAGGGGACGCCTTTAAACTGATATTGCCGTTGACTTAATGACTCGAGTCTGGTAAAGAAACCGCTGCTGCGAA ATTTGAACGCCAGCACATGGACTCGTCTACTAGCGCAGCTTAATTAACCTAGGCTGCTGCCACCGCTGAGCAATAACTAG CATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAACCTCAGGCATTTGAGAAGCACACGGTCACA CTGCTTCCGGTAGTCAATAAACCGGTAAACCAGCAATAGACATAAGCGGCTATTTAACGACCCTGCCCTGAACCGACGAC AAGCTGACGACCGGGTCTCCGCAAGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATA CATTCAAATATGTATCCGCTCATGAATTAATTCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATAT CAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATG GCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAG GTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGTTTATGCATTTCTTTCCAGACTT GTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGA GCGAGACGAAATACGCGGTCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAG CGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGA GTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTG ACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATA CAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGG AATTTAATCGCGGCCTAGAGCAAGACGTTTCCCGTTGAATATGGCTCATACTCTTCCTTTTTCAATATTATTGAAGCATT TATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGCATGCAGCGCTCTTC CGCTTCCTCGCTCACTGACTCGCTACGCTCGGTCGTTCGACTGCGGCGAGCGGTGTCAGCTCACTCAAAAGCGGTAATAC GGTTATCCACAGAATCAGGGGATAAAGCCGGAAAGAACATGTGAGCAAAAAGCAAAGCACCGGAAGAAGCCAACGCCGCA GGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGCCAGAGGTGGCGAAACCCGACAG GACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATAC CTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTTGGTATCTCAGTTCGGTGTAGGTCGT TCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGT CCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCATTGGTAACTGATTTAGAGGACTTTGTCTTGAAGTTATG CACCTGTTAAGGCTAAACTGAAAGAACAGATTTTGGTGAGTGCGGTCCTCCAACCCACTTACCTTGGTTCAAAGAGTTGG TAGCTCAGCGAACCTTGAGAAAACCACCGTTGGTAGCGGTGGTTTTTCTTTATTTATGAGATGATGAATCAATCGGTCTA TCAAGTCAACGAACAGCTATTCCGTTACTCTAGATTTCAGTGCAATTTATCTCTTCAAATGTAGCACCTGAAGTCAGCCC CATACGATATAAGTTGTAATTCTCATGTTAGTCATGCCCCGCGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCTCAA GGGCATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGT CGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGT GGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGT CCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTA TCGTCGTATCCCACTACCGAGATGTCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCAT CTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGG CACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCCAGCCAGACGCAGA CGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCGCGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAG TCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACAT TAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCG AGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTG ATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCA ACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCC CGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGAC ATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGG TTTTGCGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCCCTTATGCGACTCCTGCATTAGGAAATTAATACGACTCAC TATA



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