EPR Plasmid #3: pCasper4-Tubulin promoter-TSC1(wt)
Sequence: download sequence
| Purpose of plasmid | To make transgenic Drosophila that ubiquitously express wildtype Tsc1 |
| Depositing Lab |
Aurelio Teleman Deutsches Krebsforschungszentrum (DKFZ) |
| Depositing Scientist | Aurelio Teleman |
| Antibiotic Resistance | Ampicillin |
| Bacterial Growth Temp | 37 |
| Copy Number | High |
| Selectable Marker | Drosophila w+ |
| Reference |
Akt phosphorylates both Tsc1 and Tsc2 in Drosophila, but neither phosphorylation is required for normal animal growth. Schleich S et al. PLoS One 2009 PubMed DOI |
| MTA and Terms |
MTA (PDF download) |
How to cite this reagent:
In the Materials & Methods:
pCasper4-Tubulin promoter-TSC1(wt) was a gift from Aurelio Teleman (European Plasmid Repository plasmid #3).
In the References:
Schleich S et al.. Akt phosphorylates both Tsc1 and Tsc2 in Drosophila, but neither phosphorylation is required for normal animal growth.. PLoS One 2009 doi:10.1371/journal.pone.0006305 PubMed 19609361
pCasper4-Tubulin promoter-TSC1(wt) was a gift from Aurelio Teleman (European Plasmid Repository plasmid #3).
In the References:
Schleich S et al.. Akt phosphorylates both Tsc1 and Tsc2 in Drosophila, but neither phosphorylation is required for normal animal growth.. PLoS One 2009 doi:10.1371/journal.pone.0006305 PubMed 19609361
Sequence: download sequence
gaattcgatatcaagcttgcacaggtcctgttcgataacgtcgtactcgggaatcacctttaacttgtccgctattgggtatttgtccttaaacacattcgtaatctctcccacaatcattgccctggcctctgcaatgtaaaaagtatttataaaatatcgctggctctaaagaaaacatattaatcaaagtattgaggctttatcaatctaacatttacaagtagtcagaccacaatttttagactgcacttctgcgtaagtaaatgaaatgaaatggtgacctactcttgggtagccgtactgttatatgctgacgagccttcacctgggcctgctttttctcctgtttcgtctgatgcgtcggatgcggctggactggctgattgttgggattgggttttggatgctgctccgaaaggtgggccacagcttttgcgatggtggtgcccactatgcggttgctgcaggaactacagctgaatgttcgctttattgtctgcagctcctggggctggacgaaggccacgccctcacccggtgcggccatctcctgcggcaaaacgtatgtgggattcctgtccaagtagctctcgatgcccaccataatgcgctccagcaggttggccaggtgcgggtctagctgcggcggattcgtcgtctggttcactgagtttagggtctgcagaaagaagtgtgcctccatggtaagcggattcatctgcacgatcagcgggctttagtctaaaaattcgaatgatacaaactcgtactcacgtaaagcagtttgtttggcatcatctcggccatcgtcttattcttcttcgtggcctttgttaccggagcggctatgttgggcgcggtggagcccggaagctgaaaggttaatcgaatgcgttaacttttctgcaactcgaaagtttgccgcctttgttcgactgccaataactgttgattcgaaaattcgaatcgaagcggttgaatttcgtagggtggccaactacaccaaagttcgccggcggtgtatgcatgtgagtgtgagtgtgacagtgggtgtacgtgtgctttaagcgacacaatcgcaccgcgatgtacatatttttgtagaaatgtgtgtgcatttttcgtccggatcacggctccataaaataaatcatatcacagggctcagggatttgtgagtagaagttcccgccacttaaacgtcacttgtgaaatcgccatcgacggtcaacttttactcacactggtcttccgcggacggaccgtctcaaagtactgcctttctgcgttggaaaacatcgcctttttcgtccaaaaggagtccccaggttcgatccgcatggcgttgtgcgtgcgtgcctttcttttcaaatgattacggctattaacttgggggcgttaagttggaaacacgtaaattgcagactgcgattagagtgaccatgagtaggagttcaaaatctcctgacatcattttcttaaaacctgctttgttttttacatttctatttaatataactcctatttgaataaaaaaacaaaacaagtttagatgttaagatattaactacatcctttgctccaaagggagaggggaagttatggagttaattaatttgctgttggaaatcaatatggagtcagaaatataatgatttactaaaccttattgaatcggtaacgatgcgaatttatattaaaatagcttttatgaaacattcaacaaaaatattattaatgttggcccactttagcaaccggttaggtctaccggttgggcaagcaaagattcacgccctggttcgagtcccaactagtcctgcaaaataccgcagcaagttttagagagaccaagtgccattacctctcccacttcagttatcggttatgcggcgtttaagtcgacagcttgccgtctctagctccggtgcctatataaagcagcccgctttccacatttcatattcgttttacgtttgtcaagcctcatagccggcagttcgaacgtatacgctctctgagtcagacctcgaaatcgtagctctacacaattctgtgaattttccttgtcgcgtgtgaaacacttccaataaaaactcctgcaggtgagtactttaaaaaaaaatctagtgaaataatgctgaaaagaaatttgtgtgggcaaaattcaatgggcaaaaacgcgatgcggctttttctcaaaatggcggccggcctgcgttttttcctcaaaagtgatgacgtcatgcctgttttttttttttgttcgcaatgaggaatggctcttaaaatctactagataaaaaaaatattcattatttctatgctgctggaacgcttcattaatcttaaaaattctaaattcggttaccatgatacttcgacgcataactgtagattttggatagaattaaagagaaaatggcgagagagtaaaattccggcgtcggcaaagtagagcaaaaaaatcagtataccatttagctacctctctcactcgcacgcagtgccggctcaagttgggcgcggctctgcaattatcgattttcttggggtgtgtaactaatcatccgttttcccttcctcctcatccacagcgtgaaggtaccaaaaATGGAGCAAAAGCTCATTTCTGAAGAGGACTTGAATGAAATTGAGAAGATCATTGGTGACCTGGAGTCCAACATGACGCTGGAGAACGAGGAGGCCAAGCGCAAGCTTGTGGAGTTGCTATCCCAGAACAAGGAGCAGTGGGTGGTAAAGTTCATGCTGGACTACTTCTTTACAACTGGATCTCAGCGCATTTTGGAGGTACTGGTCAAAGCCCAGGCACCTCACGATGGGTACATCTTTGACAAGCTGGACGACTGCCTAAAGCAGTCGCAGCACCGAGTGCAGAGCCTCCAGGTGTTCTGCTTCATTGTGCGCCATCACCCTACTTGGCTGTACAAGATCGAGAAACACCGGCTGATCAAAAGTGTTTTTAAGCTTATGACGCACGAGAAGGAGATAGTTCCGCTGATGAGCGCCCTGTTGTGCATAATTACTCTGCTGCCGATCATACCGAATTCTGTGCCCAACTTTCTTAACGATCTGTTTGAGGTGTTCGGGCATTTGGCCTCGTGGAAGCTGCAGAATAGCAATAAACTGCCGGACGAGAAGCTCGTCCACCTGCAGTTGGGTCTACAGATGCTATTTCACCGCCTGTACGGCATGTATCCGTGCAGCTTTATTGCCTATTTAGTGGAGTTCATCAAGCGAGGCAACGGCGGGGGCATCTTCCAGCATACAATCAAGCCGCTGTTGAACACTGTGCGAGTGCATCCCATGCTGGTGACGGCCACGCCAGAGACTGAGGTAAACAATACGCGATGGAAGGAGATGGAGCCGCATGACGTGGTTATGGAGTGCGCCAACCTATCGCTGCCCGTCCTCTTGCCCGAGACGAGCAACGAAGACGGCAGCTATGCGTATCCCATGACGCCAGGATACAGTCGCATGACTTCAAATACCTCGAATACGGACTACAGCTATCAGCTGAGGGAGTTTCAGCAATCGAGAAATGTCTACACCCGCTTCGATTCGTTTGCCTCGGGTGATGATGTGGGTCCGATCTGGAGTCCGCATAACGAGATTGCCACGACCAGTAGCGGCATACCGCTTACACCCACCACATCGTTTATTTTGCCACTACAACCGGCTATGAACTCTCAGCTTATGGTTGGCATGACTGGCTCCTCACCGCCCGAAGCAGCTGTGGAGGCCACACCGGAAACCACCCCCTTAAAGGATATGAGGGATATCAAGCAGCCGGGACGTGCGGTCAATTCGCATGCTGTGAGAGCTATCTTCGCCGTAAGCCAGCCTTCTTCACCCATGCGCAAGGACCAGCAGAGTCAGTTCAGTTTCCCGGATGTCTCTCGCGAGGCGGAGGAGAGCAGCCACTCATATCTGGAGGTCAACAGAGGAACTGCCTATGACCGTCGCCTGTCGCAGGTCATCCAGGACAGGCATAACGTGGAGCGATCTGTAAACACACCTTGTCCAAGCAGCCTGCCAGAAATTAACTCCGATTTATCCCTTGTTGGTGGTTCCGTCTATCCATCTGTCACGCAGGAGGTCGCTGCAGTTTGTGGCGAGTGCAACGAGACGGATAGGAACCTCTGCAGTGTGGGTGGACTTCATATGCCCACCAGCCGATCCATGCACCAGCTGGCAAAGAAGCGCCGCAATCGTATGGCAAGCTACAGTGGAAATGGTTCCTGTGCGGACAGCAGAAGTTCGGCGGCAAAGAAGGCAAGTTGGAGTACTGAGGCAGAGAACCCAATGCGACGAACCAAATCCTGCTCGGCCCTTTCCGGAATGCGGCAGCAGCATCTGGAGGAGAATGATGACGAGGCCGATTGTTCGAGCCAAAGACAAAGAGGGGAGAATGGAAATACGCAAAAGACTGGCAGCCGCCTGCAGAGGAGCGGCCGGAACCTGGCCATTTCGGCGCCCAAGGATACGGCTAGAAGCTGCACCCATGCCTCCACCCAGACGGTGGAAGGACTGGACAGTGCTCCAGCGCAGTACGAGAATTGGCTTATTGAACTCCTGCTGGAGTGCAAGGAGCAAAGAATCGACTATGAAAGGAACCTTCTGTACCCGCAAGATATTCTAGACGAATACATTAAGCATGCGATCAAGGCCAATGAGTCCTTTGACGCCGAGCAGGGTCAACTGATGTGCCTACAGCTGGAATACGAAAGCTACCGTCGATCCATTCACGCAGAGCGCAATCGACGACTCATGGGGCGAAGCAGGGACAAGCGCAGCCTGGAAATGGAGCGGGATCGGTTAAGGGAGCAGCTTAAGAACTTCGATGCGAAGAACAAGGATCTGGCAAACAAAATGGATCAGGCCATTCGGTTGGCCAACGAGCGCCAGAACATCCACCAGGAGGAGCTGGGCGAAATGAGGGCTAAGTACCAGCACGAACTGGAGGAAAAGAAGTGCCTGCGGCAGGCAAACGATGACCTGCAGACGCGACTCACCAGCGAGTTGGCGCGCCACAAGGAGATGAACTATGAACTGGAGTCTCTGCGAGGTCAGGTCTTCAGTTTGGGAACCGAGCTTCAACACACCCAGCAGCAGGCGGACATTGGGCTGCAGTGCAAGCAGGAGCTGGCACGACTGGAGGCCGAGTTTATTATCATGGGTGAGGTGCAAGTGCGTTGCCGCGACCGTCTGGCTGAGATCGATAACTTCAGGGCCCGCGACGAGGAACTGCAGATGCTACAAGAGAGCAGCAACCTGGAACTGAAGGATCTGAGGCACAGCCTGGACGAGAAGACATCACAGCTGGAAAGCATGAAGCACAAGATCAGCGACCTGCAGGCCCAGCTAGCCAACAGCGAGAAGGCCATGACGGAGCAAAAGCGACTTCTAAGCACCGTCAAGGATGAGTACGAAGAAAAGTTTAAGTCCGTGAACAAGAAGTATGACGTGCAAAAGAAGATAATTATGCAGATGGAGGAGAAGCTGATGATGATGATGCAGCAGCCGCAAGGAACAACAGGTCATAACACCTGTTCCCCGGACACGGACAGAACTGACATAGCTTCATCCATTGAACGCAACTCACCGCTATCCACGTCGCTGGCCTCGAGCGAGAGCTTATCCGCCAGCCTACGCTCCACGGAGCTGAAGAACCTGCACCAGCTAGTGGACACGCCCACTATTCCGGATGTGCTGAACAGCATGGCCGGTGGCGCTCAGTTCGAGGACGAAGTGCGTCCGCCGGCCGTGGATCTGGCCTCCTCGGCAAGCACCGCCAGTGCCATCAACATCGTGCCGCACGCCTTGGACTTGCCGTCGACCTCCGGCGGCATCGGTCACACGCTCACCCACCCACATCCGCATCCGCACCTGCACCTGCAGCAACAGCAACAGGATCAACTGCAGTAGtagcagcagacgcagtttgtaaaaacaaataagaactagctttccttcgctaaacaaatgtctattgggaaaacccgcacagaaaatttacaaaaaaaagttattggttcttcatattttcatattcagttcaaaactgtaactaaggtttaataaatcgctgactccacgcaataattataaaggttataaaaaatattaatgtttgaaattttaacttgtttagcacattgcaatttgttgtaaaaaagtggttttgatacgaggcaataagtaagacgcgcattcatattataataaacatttgattaagatcagcaaacataaaaaaaaaaaaaaaaaaaaaaaCTCGAGTCTAGAGGGCCCGCGGTTCGGGCCGCataggccactagtggatctcctaggtctagagtcgacctcgaacgttaacgttaacgtaacgttaaCTCGAGCTAGATCATAATCAGCCATACCACATTTGTAAAGGTTTTACCGCGAGAAAAAACCTCCCACACCTCCCCCTGAACCTGAAACATAACTTGTCTGCGTTGTTGTTGTTAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATgtcgaggtcgacctgcagcccaagctttgcgtactcgcaaattattaaaaataaaactttaaaaataatttcgtctaattaatattatgagttaattcaaaccccacggacatgctaagggttaatcaacaatcatatcgctgtctcactcagactcaatacgacactcagaatactattcctttcactcgcacttattgcaagcatacgttaagtggatgtctcttgccgacgggaccaccttatgttatttcatcatggtctggccattctcatcgtgagcttccgggtgctcgcatatctggctctaagacttcgggcccgacgcaaggagtagccgacatatatccgaaataactgcttgtttttttttttaccattattaccatcgtgtttactgtttattgccccctcaaaaagctaatgtaattatatttgtgccaataaaaacaagatatgacctatagaatacaagtatttccccttcgaacatccccacaagtagactttggatttgtcttctaaccaaaagacttacacacctgcataccttacatcaaaaactcgtttatcgctacataaaacaccgggatatattttttatatacatacttttcaaatcgcgcgccctcttcataattcacctccaccacaccacgtttcgtagttgctctttcgctgtctcccacccgctctccgcaacacattcaccttttgttcgacgaccttggagcgactgtcgttagttccgcgcgattcggtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagcattgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccttcttcttgaactcgggctcggtgccagtatacctcaaatggttgtcgtacctctcatggttccgttacgccaacgagggtctgctgattaaccaatgggcggacgtggagccgggcgaaattagctgcacatcgtcgaacaccacgtgccccagttcgggcaaggtcatcctggagacgcttaacttctccgccgccgatctgccgctggactacgtgggtctggcccatgatgaaataacataaggtggtcccgtcgatagccgaagcttaccgaagtatacacttaaattcagtgcacgtttgcttgttgagaggaaaggttgtgtgcggacgaatttttttttgaaaacattaacccttacgtggaataaaaaaaaatgaaatattgcaaattttgctgcaaagctgtgactggagtaaaattaattcacgtgccgaagtgtgctattaagagaaaattgtgggagcagagccttgggtgcagccttggtgaaaactcccaaatttgtgatacccactttaaagattcgcagtggaaggctgcacctgcaaaaggtcagacatttaaaaggaggcgactcaacgcagatgccgtacctagtaaagtgatagagcctgaaccagaaaagataaaagaaggctataccagtgggagtacacaaacagagtaagtttgaatagtaaaaaaaatcatttatgtaaacaataacgtgactgtgcgttaggtcctgttcattgtttaatgaaaataagagcttgagggaaaaaattcgtactttggagtacgaaatgcgtcgtttagagcagcagccgaattaattctagttccagtgaaatccaagcattttctaaattaaatgtattcttattattatagttgttatttttgatatatataaacaacactattatgcccaccatttttttgagatgcatctacacaaggaacaaacactggatgtcactttcagttcaaattgtaacgctaatcactccgaacaggtcacaaaaaattaccttaaaaagtcataatattaaattagaataaatatagctgtgagggaaatatatacaaatatattggagcaaataaattgtacatacaaatatttattactaatttctattgagacgaaatgaaccactcggaaccatttgagcgaaccgaatcgcgcggaactaacgacagtcgctccaaggtcgtcgaacaaaaggtgaatgtgttgcggagagcgggtgggagacagcgaaagagcaactacgaaacgtggtgtggtggaggtgaattatgaagagggcgcgcgatttgaaaagtatgtatataaaaaatatatcccggtgttttatgtagcgataaacgagtttttgatgtaaggtatgcaggtgtgtaagtcttttggttagaagacaaatccaaagtctacttgtggggatgttcgaaggggaaatacttgtattctataggtcatatcttgtttttattggcacaaatataattacattagctttttgagggggcaataaacagtaaacacgatggtaataatggtaaaaaaaaaaacaagcagttatttcggatatatgtcggctactccttgcgtcgggcccgaagtcttagagccagatatgcgagcacccggaagctcacgatgagaatggccagacccacgtagtccagcggcagatcggcggcggagaagttaagcgtctccaggatgaccttgcccgaactggggcacgtggtgttcgacgatgtgcagctaatttcgcccggctccacgtccgcccattggttaatcagcagaccctcgttggcgtaacggaaccatgagaggtacgacaaccatttgaggtatactggcaccgagcccgagttcaagaagaagccgccaaagagcaggaatggtatgataaccggcggacccacagacagcgccatcgaggtcgaggagctggcgcaggatattagatatccgaaggacgttgacacattggccaccagagtgaccagcgccaggcagttgaagaagtgcagcactccggcccgcagtccgatcatcggataggcaatcgccgtgaagaccagtggcactgtgagaaaaagcggtaattcggcaatcgttttgcccagaaagtatgtgtcacagcgataaagtcgacttcgggcctccctcataaaaactggcagctctgaggtgaacacctaaatcgaatcgattcattagaaagttagtaaattattaatatgcaaatgtattctaaacaagacttacatttatcgtggcaaagacgttttgaaaggtcatgttggtcaggaagaggaagatggctccgttgatattcatcacgcccacttgcgtgagttgttggcccaaaaagatgaggccaatcaagatggcaaccatctgcaaattaaaatgttactcgcatctcattaatattcatatcttcaacatgttcgcgagttaaatgaaatttatttattttctgcaaaactataaactatacatctcattgaaaaaaactaagaagggtgtggaatcaggcaattctaactaaaatctagcgaatttgtttccaagaattgtaagcgttatatcatttgtttccactggaaccactcaccgttgtctgaataagtcgcacttttacgaggagtggttccttgagcaccgacagccaggatcgccacaggaccgcccggaactgcatgaaccaggtggccttgtaggtgtacccattctccggctgctccagtggcttctccaaatttttggtggccaacaactgctccatatcccgggctactttgctaatagcaaaattgtcgccatatcttggcgatccgatcacgggactcgatctcccgtccgggcacaacggccaacacctgtacgtaaaagtccgccggattgtagttggtaggacactgggcacccacgctggataggagttgagatgtaatgtaatgctagatacccttaataaacacatcgaactcactaggaaaagaagtcgacggcttcgctgggagtgcccaagaaagctaccctgccctcggccatcagaaggatcttgtcaaagagctcaaacagctcggaagacggctgatgaatggtcaggatgacggtcttgcccttctgcgacagcttcttcagcacctggacgacgctgtgggcggtaaatgagtccagtccggaggtgggctcatcgcagatcagaagcggcggatcggttagtgcctcggaggcgaatgccagacgcttcctttctccgccggacagacctttcaccctgccgggcacaccgatgatcgtgtgctgacatttgctgagcgaaagctcctggatcacctgatccacgcgggccactcgctgccgataggtcagatgtcgtggcatccgcaccatggcctggaaaatcaggtgttccctggccgttagggagccgataaagaggtcatcctgctggacataggcgcacctggcctgcatctccttggcgtccacaggttggccattgagcagtcgcatcccggatggcgatacttggatgccctgcggcgatcgaaaggcaagggcattcagcagggtcgtctttccggcaccggaactgcccatcacggccaaaagttcgcccggataggccacgccgcaaactgagtttcaaattggtaattggaccctttattaagatttcacacagatcagccgactgcgaatagaaactcaccgttcttgagcaaatgtttcctgggcgccggtatgtgtcgctcgttgcagaatagtccgcgtgtccggttgaccagctgccgccatccggagcccggctgattgaccgccccaaagatgtccatattgtgccaggcataggtgaggttctcggctagttggccgctccctgaaccggagtcctccggcggactgggtggcaggagcgtgccgtagtttttggcctgcccgaagccctggttaatgcagctctgcgaagcgtccgctgtcaccctgcaatgataggggatctcaaatatcaactacaagcgttatgctcatctaaccccgaacaaaacgaagtatcctacgaagtaggtttatacttttatttattttttgtgcatctaggatcagcttaaaatatctggttgttatattttttgtaaaaaagaatgtagtcgaaaatgaatgcctttagatgtcttgatcatgatatgatcttaaaaattgtcttatatagcgagcacagctaccagaataatctgtttcgtgtcactatttgtttgtgcgattgcggtttgggatttttgtgggtcgcagttctcacgccgcagacaatttgatgttgcaatcgcagttcctatagatcaagtgaacttaagatgtatgcacatgtactactcacattgttcagatgctcggcagatgggtgtttgctgcctccgcgaattaatagctcctgatcctcttggcccattgccgggatttttcacactttcccctgcttacccacccaaaaccaatcaccaccccaatcactcaaaaaacaaacaaaaataagaagcgagaggagttttggcacagcactttgtgtttaattgatggcgtaaaccgcttggagcttcgtcacgaaaccgctgacaaagtgcaactgaaggcggacattgacgctaggtaacgctacaaacggtggcgaaagagatagcggacgcagcggcgaaagagacggcgatatttctgtggacagagaaggaggcaaacagcgctgactttgagtggaatgtcattttgagtgagaggtaatcgaaagaacctggtacttcaaatacccttggatcgaagtaaatttaaaactgatcagataagttcaatgatatccagtgcagtaaaaaataaaaaaaaaatatgtttttttaaatctacattctccaaaaaagggttttattaacttacatacatacta
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