EPR Plasmid #272: pYM29
Sequence: download sequence
| Purpose of plasmid | PCR template for C-terminal EGFP: marker pKlTRP1-KlTRP1-tKlTRP1, K. lactis TRP1 complements the trytophane auxotrophy of a S.cerevisiae trp1 mutant. |
| Depositing Lab |
Michael Knop Heidelberg University |
| Depositing Scientist | Daniel Kirrmaier |
| Antibiotic Resistance | Ampicillin |
| Bacterial Growth Temp | 37 |
| Copy Number | High |
| Selectable Marker | |
| Reference |
A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes. Janke C et al. Yeast 2004 PubMed |
| MTA and Terms |
MTA (PDF download) |
How to cite this reagent:
In the Materials & Methods:
pYM29 was a gift from Michael Knop (European Plasmid Repository plasmid #272).
In the References:
Janke C et al.. A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes.. Yeast 2004 PubMed 15334558
pYM29 was a gift from Michael Knop (European Plasmid Repository plasmid #272).
In the References:
Janke C et al.. A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes.. Yeast 2004 PubMed 15334558
Sequence: download sequence
gaacgcggccgccagctgaagcttcgtacgctgcaggtcgacggagcaggtgctggtgctggtgctggagcaatgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaaggatcctatccatatgacgttccagattacgcttctagctaaaaagtggaacgatcattcactatatatatatcaatttatatatacgtatgtgtaattgaagaaagatacgttttttcctctattgagaggcctgctggatgaatagctttaccttttctaaatccttgataccatcagtctctactcctccacttacatcgacaccgatcgcatttggtaacatattaatggcaacagaaacgttatcaggattcaatccaccagcgataatgaattttatctcgggatgacttgcagaccaactggaaattgcactccaattcaatttctcaccagtgccaccttcaccagaatcgaacaacgtcagcacattgtctacgtgttcatacaggtccagtagtaattcacaatcctgtgggaactggaacctcttaatgattggaattgaagatgggatcaaagatctgtattctttaatatcttcatctccatgtaattgtatcacatctagattatattcgtggtacagttgaaggacatcatcaacggactgatttctaaacaccccgactagtttagtacctttcacgttctcttgttggtgaactgcagttgaaatacctttcgcaacagatgagtcaatggttcttttcctaccgggaacacaaatgatacctaagtaatcagcaccatcatccacagcagtctttgcagcttcaacggtttgcaaaccacacactttaacgagcatcacagtagatttcgcaagagggtagctcgctcagagtacccaagtaaatgattagtaaactgatgtttgatagttcaatttttcaatgaaataaccttatattaaaattgatattactattatacaaaaataaagaataaaggatttgagtttatacataaaataccattattatttgttcagtgagagataccggggtatatgggatgtgtgtagtgataccatgcaatcatgtatcaaacatggaagaagtgaagggggagaaagaaacttgaaaaaaagaaaaccatttgaaaagaggaaaagtatctaattaagatctttcacctcttagtcttctggcctcgaggcgagctcgaattcatcgatgatatcagatccactagtggcctatgcggccgcggatctgccggtctccctatagtgagtcgtattaatttcgataagccaggttaacctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcaatgctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggccctttcgtctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggctggcttaactatgcggcatcagagcagattgtactgagagtgcaccatatggacatattgtcgttagaacgcggctacaattaatacataaccttatgtatcatacacatacgatttaggtgacactata
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