In the Materials & Methods:
pHD1701 was a gift from Christine Clayton (European Plasmid Repository plasmid #196).

In the References:
Voncken F et al.. Depletion of GIM5 causes cellular fragility, a decreased glycosome number, and reduced levels of ether-linked phospholipids in trypanosomes.. J Biol Chem 2003 PubMed 12829709

GAATTCGAGCTCATATAGTTGGTATGTATTCTAATTCCAGACTACTGGCG TGGGATAAACATGTCCCCCTGATTAAAAGGAAAGATTCCATAGCCCATTA GTGCAAAGATAATTGGTACACCGTCAAAAACACATGGGAGACCACACGAT ACGACACTGTGACCGTAGCATCAATTCCGCACTCGATATAACTCTATCGA AGAACTTCCATGGTACAAACTGGTCGCCACGGTCTGCTCCACACGGAGAT CATCGTATCATTTTTATCGATAGCGGCCGCTATCGATGTATGCCTTGGCC CTGATGGCATGCCAATTTCACTACAACGGACTATGTGGACCCCGTTATCA TGGAAATGCGCTAGTTGGAGGAAGTTAGACCGCGCCGGAAAAGAGAGGGG TAGAGAAAATGACAACTTGGAAGATATCCACACACGCACGGTGAAACGTT AGCAACAATTATTAGGGAAGCACGCTTGCCCTAGTCCCACGAGTAACCAA GACTCCAAAAGCCTTTCTGGCACAGAGAGCGAGCCGAAATGGAAAAGAGA AACAATGCCTGCACTAACACTACTGAGCGATTCGCCTCGCCGCGGAGGAC CGAATACTAATAACGACACTTGCGGTCAAAAAGTAGAAGAACAAATGCTC AACGATGAGTGAATCAGGTTAGGGTAGTTGGAAAATTATACAGAATGTCT TTGGCAACACACCGGTACCGTCATTGGGGTTAAGCGGAAAGGTGTGTGTC AGTAGGTTGTGAGGTGAAAGCGTTTTCAGATGCATAGTGAGCTTAATGTC CTTTTCACAGTATATCGTGTCTGATAGGTATCTCTTATTAGTATAGTCGA ATACTAGTCAATAGTGCGTTTTGTGCAAAATGTCCATTTTGTGGCAGTGA TGGGGTTGTTTTATGCTATTCCGTGTCTCTGGGTGGGCGTGCATTGAAAA TAGGGGTTATCGGGTagggatctccctatcagtgatagagatctccctat cagtgatagagatccctGAGTACTGAGTTTAACATGTTCTCGTCCCGGGC TGCACGCGCCTTCGAGTTTTTTTTCCTTTTCCCCATTTTTTTCAACTTGA AGACTTCAATTACACCAAAAAGTAAAATTCACATGGGCGAGCAAAAGCTC ATTTCTGAAGAGGACTTGGGCGGAGGCTTAATTAACGGCGAGCAAAAGCT CATTTCTGAAGAGGACTTGAAGCTTcgcggggcccacgcgttctagagtt aacGGATCCTAACACCGGGTTGTGTGGCCAAAATTGTTCTGTAGTCGCTG TGAGTTGACACGGCTAGTGCTTATGATTTTCCTCGCGTGTGGTGCCTGTA CTCAGCCCTATGCCTTATTTGCAACACATTTACGTACAGCGCACAAGAGA AGAGAAGATCACTTGAAGATAATAAATATAGGGTTGTAGGCATCTTGTTT AACTCAAATTTTCTCGTCTTGGTGTGTCGACATGATTGAAATAGTGCCAC CAGTTGTGTTTGATGCGTTTGTTATCTATGCAGTATTCTGCAGTTTAATT TGTTGGATGAGCTATTTCATTAATTTTTTTGCCTTCTCTTCTTTTGGGTT CGCTCGAGTTTCCACCCAGCGCGGGTGCATTCTGGCTCTTATATATACTT ATTGTCATGACAGAGTATATTGTACTGTGTTGATAAGGGACGGGTAACTG TATTGAAGAGCCGATGCTTTTGACATGTTAGATATAATATGTTTTATTGT AAAGTCAATACAACACACAATAGGATAATAATGATAAAGTTAAAAAAGTA TATATAGTAATAGAAATATATCTTATATAGGAAAGATTAAGCAGTAAAAG TAGCGCTTACGGCGTACGGAGCAGGAGAGCAACTGACCGCTCTCAGAgcc cGGGCACAGCAAGGTCTTCTGAAATTCATGTTTTTTTTTTTTTTACTCTG CATTGCAGTCTCCGCTCTTATTTAGTTTTGCTTTACGTAAGGTCTCGTTG CTGCCATAAAATAAGCTctagaACTAGTGATGAAAAAGCCTGAACTCACC GCGACGTCTGTCGAGAAGTTTCTGATCGAAAAGTTCGACAGCGTCTCCGA CCTGATGCAGCTCTCGGAGGGCGAAGAATCTCGTGCTTTCAGCTTCGATG TAGGAGGGCGTGGATATGTCCTGCGGGTAAATAGCTGCGCCGATGGTTTC TACAAAGATCGTTATGTTTATCGGCACTTTGCATCGGCCGCGCTCCCGAT TCCGGAAGTGCTTGACATTGGGGAATTCAGCGAGAGCCTGACCTATTGCA TCTCCCGCCGTGCACAGGGTGTCACGTTGCAAGACCTGCCTGAAACCGAA CTGCCCGCTGTTCTGCAGCCGGTCGCGGAGGCCATGGATGCGATCGCTGC GGCCGATCTTAGCCAGACGAGCGGGTTCGGCCCATTCGGACCGCAAGGAA TCGGTCAATACACTACATGGCGTGATTTCATATGCGCGATTGCTGATCCC CATGTGTATCACTGGCAAACTGTGATGGACGACACCGTCAGTGCGTCCGT CGCGCAGGCTCTCGATGAGCTGATGCTTTGGGCCGAGGACTGCCCCGAAG TCCGGCACCTCGTGCACGCGGATTTCGGCTCCAACAATGTCCTGACGGAC AATGGCCGCATAACAGCGGTCATTGACTGGAGCGAGGCGATGTTCGGGGA TTCCCAATACGAGGTCGCCAACATCTTCTTCTGGAGGCCGTGGTTGGCTT GTATGGAGCAGCAGACGCGCTACTTCGAGCGGAGGCATCCGGAGCTTGCA GGATCGCCGCGGCTCCGGGCGTATATGCTCCGCATTGGTCTTGACCAACT CTATCAGAGCTTGGTTGACGGCAATTTCGATGATGCAGCTTGGGCGCAGG GTCGATGCGACGCAATCGTCCGATCCGGAGCCGGGACTGTCGGGCGTACA CAAATCGCCCGCAGAAGCGCGGCCGTCTGGACCGATGGCTGTGTAGAAGT ACTCGCCGATAGTGGAAACCGACGCCCCAGCACTCGTCCGAGGGCAAAGG AATAGAGTAGATGCCGACCGGGATCGATCCCCCGATCCTAACACCGGGTT GTGTGGCCAAAATTGTTCTGTAGTCGCTGTGAGTTGACACGGCTAGTGCT TATGATTTTCCTCGCGTGTGGTGCCTGTACTCAGCCCTATGCCTTATTTG CAACACATTTACGTACAGCGCACAAGAGAAGAGAAGATCACTTGAAGATA ATAAATATAGGGTTGTAGGCATCTTGTTTAACTCAAATTTTCTCGTCTTG GTGTGTCGACATGATTGAAATAGTGCCACCAGTTGTGTTTGATGCGTTTG TTATCTATGCAGTATTCTGCAAGGCCTTGCAAGGCCTTGCAGGCATGCAA GCTAGCTTGTATTCTATAGTGTCACCTAAATCGTATGTGTATGATACATA AGGTTATGTATTAATTGTAGCCGCGTTCTAACGACAATATGTACAAGCCT AATTGTGTAGCATCTGGCTTACTGAAGCAGACCCTATCATCTCTCTCGTA AACTGCCGTCAGAGTCGGTTTGGTTGGACGAACCTTCTGAGTTTCTGGTA ACGCCGTTCCGCACCCCGGAAATGGTCAGCGAACCAATCAGCAGGGTCAT CGCTAGCCAGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCG CCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAA GATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTAT GGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCACC ATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCT TCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGATATGGTGCACTCTCA GTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCA ACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTA CAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCAC CGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTT TTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCAC TTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATAC ATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAA TAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCT TATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAA CGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGT TACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCC CGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCG CGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATA CACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCA TCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCA TGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCG AAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCT TGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTG ACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACT GGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGA GGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCT GGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATC ATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTA CACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTG AGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTAC TCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGAT CTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTG AGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCT TCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAA ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTC TTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTC CTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACC GCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTG GCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGAT AAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTT GGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAG AAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGC GGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGC CTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTC GATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGC AACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACAT GTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCT TTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAG TCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCC CGCGCGTTGGCCGATTCATTAATGCAGCTGGCTTATCGAAATTAATACGA CTCACTATAGGGAGACCG