In the Materials & Methods:
pHD1700 was a gift from Christine Clayton (European Plasmid Repository plasmid #195).

In the References:
Voncken F et al.. Depletion of GIM5 causes cellular fragility, a decreased glycosome number, and reduced levels of ether-linked phospholipids in trypanosomes.. J Biol Chem 2003 PubMed 12829709

GAATTCGAGCTCATATAGTTGGTATGTATTCTAATTCCAGACTACTGGCG TGGGATAAACATGTCCCCCTGATTAAAAGGAAAGATTCCATAGCCCATTA GTGCAAAGATAATTGGTACACCGTCAAAAACACATGGGAGACCACACGAT ACGACACTGTGACCGTAGCATCAATTCCGCACTCGATATAACTCTATCGA AGAACTTCCATGGTACAAACTGGTCGCCACGGTCTGCTCCACACGGAGAT CATCGTATCATTTTTATCGATAGCGGCCGCTATCGATGTATGCCTTGGCC CTGATGGCATGCCAATTTCACTACAACGGACTATGTGGACCCCGTTATCA TGGAAATGCGCTAGTTGGAGGAAGTTAGACCGCGCCGGAAAAGAGAGGGG TAGAGAAAATGACAACTTGGAAGATATCCACACACGCACGGTGAAACGTT AGCAACAATTATTAGGGAAGCACGCTTGCCCTAGTCCCACGAGTAACCAA GACTCCAAAAGCCTTTCTGGCACAGAGAGCGAGCCGAAATGGAAAAGAGA AACAATGCCTGCACTAACACTACTGAGCGATTCGCCTCGCCGCGGAGGAC CGAATACTAATAACGACACTTGCGGTCAAAAAGTAGAAGAACAAATGCTC AACGATGAGTGAATCAGGTTAGGGTAGTTGGAAAATTATACAGAATGTCT TTGGCAACACACCGGTACCGTCATTGGGGTTAAGCGGAAAGGTGTGTGTC AGTAGGTTGTGAGGTGAAAGCGTTTTCAGATGCATAGTGAGCTTAATGTC CTTTTCACAGTATATCGTGTCTGATAGGTATCTCTTATTAGTATAGTCGA ATACTAGTCAATAGTGCGTTTTGTGCAAAATGTCCATTTTGTGGCAGTGA TGGGGTTGTTTTATGCTATTCCGTGTCTCTGGGTGGGCGTGCATTGAAAA TAGGGGTTATCGGGTagggatctccctatcagtgatagagatctccctat cagtgatagagatccctGAGTACTGAGTTTAACATGTTCTCGTCCCGGGC TGCACGCGCCTTCGAGTTTTTTTTCCTTTTCCCCATTTTTTTCAACTTGA AGACTTCAATTACACCAAAAAGTAAAATTCACAAGCTTcgcggggcccac gcgttctagagttaacGGATCCaacgagcaaaagctcatttctgaagagg acttgttaattaacgagcaaaagctcatttctgaagaggacttgTAACAC CGGGTTGTGTGGCCAAAATTGTTCTGTAGTCGCTGTGAGTTGACACGGCT AGTGCTTATGATTTTCCTCGCGTGTGGTGCCTGTACTCAGCCCTATGCCT TATTTGCAACACATTTACGTACAGCGCACAAGAGAAGAGAAGATCACTTG AAGATAATAAATATAGGGTTGTAGGCATCTTGTTTAACTCAAATTTTCTC GTCTTGGTGTGTCGACATGATTGAAATAGTGCCACCAGTTGTGTTTGATG CGTTTGTTATCTATGCAGTATTCTGCAGTTTAATTTGTTGGATGAGCTAT TTCATTAATTTTTTTGCCTTCTCTTCTTTTGGGTTCGCTCGAGTTTCCAC CCAGCGCGGGTGCATTCTGGCTCTTATATATACTTATTGTCATGACAGAG TATATTGTACTGTGTTGATAAGGGACGGGTAACTGTATTGAAGAGCCGAT GCTTTTGACATGTTAGATATAATATGTTTTATTGTAAAGTCAATACAACA CACAATAGGATAATAATGATAAAGTTAAAAAAGTATATATAGTAATAGAA ATATATCTTATATAGGAAAGATTAAGCAGTAAAAGTAGCGCTTACGGCGT ACGGAGCAGGAGAGCAACTGACCGCTCTCAGAgcccGGGCACAGCAAGGT CTTCTGAAATTCATGTTTTTTTTTTTTTTACTCTGCATTGCAGTCTCCGC TCTTATTTAGTTTTGCTTTACGTAAGGTCTCGTTGCTGCCATAAAATAAG CTctagaACTAGTGATGAAAAAGCCTGAACTCACCGCGACGTCTGTCGAG AAGTTTCTGATCGAAAAGTTCGACAGCGTCTCCGACCTGATGCAGCTCTC GGAGGGCGAAGAATCTCGTGCTTTCAGCTTCGATGTAGGAGGGCGTGGAT ATGTCCTGCGGGTAAATAGCTGCGCCGATGGTTTCTACAAAGATCGTTAT GTTTATCGGCACTTTGCATCGGCCGCGCTCCCGATTCCGGAAGTGCTTGA CATTGGGGAATTCAGCGAGAGCCTGACCTATTGCATCTCCCGCCGTGCAC AGGGTGTCACGTTGCAAGACCTGCCTGAAACCGAACTGCCCGCTGTTCTG CAGCCGGTCGCGGAGGCCATGGATGCGATCGCTGCGGCCGATCTTAGCCA GACGAGCGGGTTCGGCCCATTCGGACCGCAAGGAATCGGTCAATACACTA CATGGCGTGATTTCATATGCGCGATTGCTGATCCCCATGTGTATCACTGG CAAACTGTGATGGACGACACCGTCAGTGCGTCCGTCGCGCAGGCTCTCGA TGAGCTGATGCTTTGGGCCGAGGACTGCCCCGAAGTCCGGCACCTCGTGC ACGCGGATTTCGGCTCCAACAATGTCCTGACGGACAATGGCCGCATAACA GCGGTCATTGACTGGAGCGAGGCGATGTTCGGGGATTCCCAATACGAGGT CGCCAACATCTTCTTCTGGAGGCCGTGGTTGGCTTGTATGGAGCAGCAGA CGCGCTACTTCGAGCGGAGGCATCCGGAGCTTGCAGGATCGCCGCGGCTC CGGGCGTATATGCTCCGCATTGGTCTTGACCAACTCTATCAGAGCTTGGT TGACGGCAATTTCGATGATGCAGCTTGGGCGCAGGGTCGATGCGACGCAA TCGTCCGATCCGGAGCCGGGACTGTCGGGCGTACACAAATCGCCCGCAGA AGCGCGGCCGTCTGGACCGATGGCTGTGTAGAAGTACTCGCCGATAGTGG AAACCGACGCCCCAGCACTCGTCCGAGGGCAAAGGAATAGAGTAGATGCC GACCGGGATCGATCCCCCGATCCTAACACCGGGTTGTGTGGCCAAAATTG TTCTGTAGTCGCTGTGAGTTGACACGGCTAGTGCTTATGATTTTCCTCGC GTGTGGTGCCTGTACTCAGCCCTATGCCTTATTTGCAACACATTTACGTA CAGCGCACAAGAGAAGAGAAGATCACTTGAAGATAATAAATATAGGGTTG TAGGCATCTTGTTTAACTCAAATTTTCTCGTCTTGGTGTGTCGACATGAT TGAAATAGTGCCACCAGTTGTGTTTGATGCGTTTGTTATCTATGCAGTAT TCTGCAAGGCCTTGCAAGGCCTTGCAGGCATGCAAGCTAGCTTGTATTCT ATAGTGTCACCTAAATCGTATGTGTATGATACATAAGGTTATGTATTAAT TGTAGCCGCGTTCTAACGACAATATGTACAAGCCTAATTGTGTAGCATCT GGCTTACTGAAGCAGACCCTATCATCTCTCTCGTAAACTGCCGTCAGAGT CGGTTTGGTTGGACGAACCTTCTGAGTTTCTGGTAACGCCGTTCCGCACC CCGGAAATGGTCAGCGAACCAATCAGCAGGGTCATCGCTAGCCAGATCCT CTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTG CTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCAC TTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGT GGCCGGGGGACTGTTGGGCGCCATCTCCTTGCACCATTCCTTGCGGCGGC GGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGT CGCATAAGGGAGAGCGTCGATATGGTGCACTCTCAGTACAATCTGCTCTG ATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCG CCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGAC CGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAAC GCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTC ATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGT GCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATC CGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGG AAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGC GGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAA AAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGAT CTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCC AATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTA TTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAAT GACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCAT GACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTG CGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCT TTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACC GGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTG TAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACT CTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGC AGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATA AATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGG CCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCA GGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCAC TGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAG ATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCT TTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACT GAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTT TTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGC GGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAA CTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCG TAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGC TCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTC TTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCG GGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTA CACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTC CCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACA GGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAG TCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCT CGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTA CGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTT ATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATA CCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAA GCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGAT TCATTAATGCAGCTGGCTTATCGAAATTAATACGACTCACTATAGGGAGA CCG