EPR Plasmid #12: pMT3b-REPTOR-HA
Sequence: download sequence
| Purpose of plasmid | Copper-inducible expression of HA-tagged Drosophila REPTOR in fly cell culture |
| Depositing Lab |
Aurelio Teleman Deutsches Krebsforschungszentrum (DKFZ) |
| Depositing Scientist | Aurelio Teleman |
| Antibiotic Resistance | Ampicillin |
| Bacterial Growth Temp | 37 |
| Copy Number | High |
| Selectable Marker | |
| Reference |
REPTOR and REPTOR-BP Regulate Organismal Metabolism and Transcription Downstream of TORC1. Tiebe M et al. Dev Cell 2015 PubMed DOI |
| MTA and Terms |
MTA (PDF download) |
How to cite this reagent:
In the Materials & Methods:
pMT3b-REPTOR-HA was a gift from Aurelio Teleman (European Plasmid Repository plasmid #12).
In the References:
Tiebe M et al.. REPTOR and REPTOR-BP Regulate Organismal Metabolism and Transcription Downstream of TORC1.. Dev Cell 2015 doi:10.1016/j.devcel.2015.03.013 PubMed 25920570
pMT3b-REPTOR-HA was a gift from Aurelio Teleman (European Plasmid Repository plasmid #12).
In the References:
Tiebe M et al.. REPTOR and REPTOR-BP Regulate Organismal Metabolism and Transcription Downstream of TORC1.. Dev Cell 2015 doi:10.1016/j.devcel.2015.03.013 PubMed 25920570
Sequence: download sequence
ggcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgacatgattaccgttgcaggacaggatgtggtgcccgatgtgactagctctttgctgcaggccgtcctatcctctggttccgataagagacccagaactccggccccccaccgcccaccgccacccccatacatatgtggtacgcaagtaagagtgcctgcgcatgccccatgtgccccaccaagagttttgcatcccatacaagtccccaaagtggagaaccgaaccaattcttcgcgggcagaacaaaagcttctgcacacgtctccactcgaatttggagccggccggcgtgtgcaaaagaggtgaatcgaacgaaagacccgtgtgtaaagccgcgtttccaaaatgtataaaaccgagagcatctggccaatgtgcatcagttgtggtcagcagcaaaatcaagtgaatcatctcagtgcaactaaagggaattcATGACAGAGAATCAGCTGTATCCCATGTCATCGGAGTTCTTTGACACCGGCTCGAACAGCAGCAGCAACACACTTAAATACGATCTGTATTCCCTGGGCTCCACCGTTGTGGGGGCGGCGCTGCCCACGTTGACGATCAACACGGGCTACGGCAGCAGCAGCAGCAACAATAACAACACTAATAACAACAATAACAACAGCAGTAGTCACAGCAGCAGCAGCAACTGCTCCACCAGCACCACAAATACCTGTAATGTAATGCTAAGTACAACGGCCATAACAATACCACGCAGCAGCAACCACAACCAGATCCATCATCATCCGTATCAGCAGTCCGATTTGCAGACACCGCGCCATCATCCCAGTCCTCTACACACGTTGCCCAGCATGACCCATCAGCAAAATCAGCTTCAGCAGCAGCAGCAGCAGCAACACCACAACCAACAGCAGCAGCTGCAGCAATCGCATTTGGCACTGGGAGAGCTTTCAGACTTTGGACTAGATGCACTGGATGCCGCTTCGCTATCGCCCACTCTGCTGCAGGATGTGAGCCTGAGTGCTGTTTCTCCTCTAAGCACCACACTCTACAATGGCAACACCAGTGGAGCAGGCAGCAGCAACGGAATAGGATCCGGCAGTGGTGGCTACTTCACGCCCGACATGTCGCACAGCCTCAGCCTGAATGTGGTCAGTGAGCAGGTTCTGCTGCAGGAAGCCACAACCCCGAATGAGTTGCTCTACGAGATGACACCCAACTCGAATGCCATGTGGAGTGATATCAGCAGTGCCATTATCCATACCAAACACGAGCCATTCAGTCTGGACGATGACTATATCTTTCCCAACGATAAGGCCGAAATACAGGCAGCCGATTTGTCTGACCTTAATGGCGGCGATTTTCTGGATGTGATCGGAAATATAGAGGATTTCTTGCCGCAGACCGCAGTCACTCAAAGTGTCAATTTCCTACTGTCGCCGCAAGCTCAAGGACAGGATGCCCTGGTGGCACCGCCCATGGAACTTTTGCAACAGCAACAACAGAATCACCAGCAGCTGCAGGTCGGCAGCCTGCCGCAGTTGCAAACGCTGCTCACGCTTTCCCAGCAGCAGCAGTCGAACAGTTCCTCCACTAGTCCGTATGAGATCTATCACAGTACGCCCCAAAAGCCGCAGCAGCAACAGCTGTCCGCCAGCTTCTCGCCCGGCAGTCAGGCATCCCAATCGCCATTGACCCCACCGCCGCCACCGCACGCCAATCGACCCCAGTATCAGATGGTTAAGTCGCGCAACATGCAGGAATTGATCAAGAAGGGCTTTCCCATGTCCTCGCCGCCAGAACGCTCGATCCTAAGTCAGTCCGCCGCCTTGAGTCCGGGTGGTAGCAGTGGTTTTGGCAGCTCGGCTTCTGGCAACAGTACAACTACCAGTAATCAGACATCCGGATCAGCCGTGCGCAAATCGTTTGGCTACCAGAGCGCCGTTGAGAACTCCCAGCTGAGTCGCCTCAGCTCGTCGGCTCCGACGCACTTGGGATTGGAGCACATCTGGATGCGGCGGGAGCCACGACAGCATTTACTCTCCACCGGCTCGCTGGCCGAGGCCGAGAGCTTCTCTTCACTGAGCACGGGCAGTGTCTTGTCCCCGGATGGCATTGACTTCTCGCAGGACGATGAGGATGACAATTCGAGTGAGAATAGCGATAACTACGACGATTGTAGCAGCGACAATGGACTCTCTGAGGATGAAGATGAAACGCGTACCTCGACACCAAACCATTTGTCCAGTAGCAAGGGCAAAGAGCGATTTTTCTGGCAGTACAATGTTCAGGCCAAAGGACCCAAGGGAAAGAGACTGGTCTTTCAATCGAAGCTCGAGGATCCTCATGTGCTGAACGAGGTGACAGACCCCGTCTTCAGTCCAACGTGCTCCGTGCGAGGCATTAAGGTGTACAAGCACAGTGGCAAGGCCCGCAAGGGCGATGGTAACGATTTAACTCCCAATGCCAGGAAGCTGCACAATATTGGCAAGGAACTGGACAAGCTAAGCCGCACCATCAATGACATGACTCCTGTTTCCGAGCTGCCATTCAATGTGCGCCCCAAGTCGCGCAAGGAGAAGAACAAGCTGGCCTCACGCGCCTGTCGCCTGAAGAAGAAGGCGCAGCACGAGGCCAACAAAATCAAGTTGTTCGGCCTTGAAATTGAACACAAGCGCCTCATGAATGGTATAGCCGAGCTAAAGCAGGCCTTGGTCGTCAAGCATCGCACCAAGAACCTGGGCGAATCCACCGAGGAGGTGGACCAACAAATTGCACGCATTTACGCTACCGCCTCTTCTGGAATCCGCATTGCGGGTGGTTCAACGGATTTTGTGAACAAGGTGCTGGAGAATATGCGCGGCGGCATGCCGAATGGAGGCCTCGAGGAGCTGCGTAAATCGTCGggatcctacccctacgacgtgcccgactacgcctaagtcgacctgcaggcatgcaagctattcgatgcacactcacattcttctcctaatacgataataaaactttccatgaaaaatatggaaaaatatatgaaaattgagaaatccaaaaaactgataaacgctctacttaattaaaatagataaatgggagcggcaggaatggcggagcatggccaagttcctccgccaatcagtcgtaaaacagaagtcgtggaaagcggatagaaagaatgttcgatttgacgggcaagcatgtctgctatgtggcggattgcggaggaattgcactggagaccagcaaggttctcatgaccaagaatatagcggtgagtgagcgggaagctcggtttctgtccagatcgaactcaaaactagtccagccagtcgctgtcgaaactaattaagtaaatgagtttttcatgttagtttcgcgctgagcaacaattaagtttatgtttcagttcggcttagatttcgctgaaggacttgccactttcaatcaatactttagaacaaaatcaaaactcattctaatagcttggtgttcatctttttttttaatgataagcattttgtcgtttatactttttatatttcgatattaaaccacctatgaagttcattttaatcgccagataagcaatatattgtgtaaatatttgtattctttatcaggaaattcagggagacggggaagttactatctactaaaagccaaacaatttcttacagttttactctctctactctagagtagctt
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