EPR Plasmid #1: pTubulin-EGFP
Sequence: download sequence
Purpose of plasmid | To express EGFP ubiquitously in Drosophila |
Depositing Lab |
Aurelio Teleman Deutsches Krebsforschungszentrum (DKFZ) |
Depositing Scientist | Aurelio Teleman |
Antibiotic Resistance | Ampicillin |
Bacterial Growth Temp | 37 |
Copy Number | High |
Selectable Marker | Drosophila w+ |
Reference |
Insulin/IGF signaling drives cell proliferation in part via Yorkie/YAP. Straßburger K et al. Dev Biol 2012 PubMed DOI |
MTA and Terms |
MTA (PDF download) |
How to cite this reagent:
In the Materials & Methods:
pTubulin-EGFP was a gift from Aurelio Teleman (European Plasmid Repository plasmid #1).
In the References:
Straßburger K et al.. Insulin/IGF signaling drives cell proliferation in part via Yorkie/YAP.. Dev Biol 2012 doi:10.1016/j.ydbio.2012.05.008 PubMed 22609549
pTubulin-EGFP was a gift from Aurelio Teleman (European Plasmid Repository plasmid #1).
In the References:
Straßburger K et al.. Insulin/IGF signaling drives cell proliferation in part via Yorkie/YAP.. Dev Biol 2012 doi:10.1016/j.ydbio.2012.05.008 PubMed 22609549
Sequence: download sequence
gaattcgatatcaagcttgcacaggtcctgttcgataacgtcgtactcgggaatcacctttaacttgtccgctattgggtatttgtccttaaacacattcgtaatctctcccacaatcattgccctggcctctgcaatgtaaaaagtatttataaaatatcgctggctctaaagaaaacatattaatcaaagtattgaggctttatcaatctaacatttacaagtagtcagaccacaatttttagactgcacttctgcgtaagtaaatgaaatgaaatggtgacctactcttgggtagccgtactgttatatgctgacgagccttcacctgggcctgctttttctcctgtttcgtctgatgcgtcggatgcggctggactggctgattgttgggattgggttttggatgctgctccgaaaggtgggccacagcttttgcgatggtggtgcccactatgcggttgctgcaggaactacagctgaatgttcgctttattgtctgcagctcctggggctggacgaaggccacgccctcacccggtgcggccatctcctgcggcaaaacgtatgtgggattcctgtccaagtagctctcgatgcccaccataatgcgctccagcaggttggccaggtgcgggtctagctgcggcggattcgtcgtctggttcactgagtttagggtctgcagaaagaagtgtgcctccatggtaagcggattcatctgcacgatcagcgggctttagtctaaaaattcgaatgatacaaactcgtactcacgtaaagcagtttgtttggcatcatctcggccatcgtcttattcttcttcgtggcctttgttaccggagcggctatgttgggcgcggtggagcccggaagctgaaaggttaatcgaatgcgttaacttttctgcaactcgaaagtttgccgcctttgttcgactgccaataactgttgattcgaaaattcgaatcgaagcggttgaatttcgtagggtggccaactacaccaaagttcgccggcggtgtatgcatgtgagtgtgagtgtgacagtgggtgtacgtgtgctttaagcgacacaatcgcaccgcgatgtacatatttttgtagaaatgtgtgtgcatttttcgtccggatcacggctccataaaataaatcatatcacagggctcagggatttgtgagtagaagttcccgccacttaaacgtcacttgtgaaatcgccatcgacggtcaacttttactcacactggtcttccgcggacggaccgtctcaaagtactgcctttctgcgttggaaaacatcgcctttttcgtccaaaaggagtccccaggttcgatccgcatggcgttgtgcgtgcgtgcctttcttttcaaatgattacggctattaacttgggggcgttaagttggaaacacgtaaattgcagactgcgattagagtgaccatgagtaggagttcaaaatctcctgacatcattttcttaaaacctgctttgttttttacatttctatttaatataactcctatttgaataaaaaaacaaaacaagtttagatgttaagatattaactacatcctttgctccaaagggagaggggaagttatggagttaattaatttgctgttggaaatcaatatggagtcagaaatataatgatttactaaaccttattgaatcggtaacgatgcgaatttatattaaaatagcttttatgaaacattcaacaaaaatattattaatgttggcccactttagcaaccggttaggtctaccggttgggcaagcaaagattcacgccctggttcgagtcccaactagtcctgcaaaataccgcagcaagttttagagagaccaagtgccattacctctcccacttcagttatcggttatgcggcgtttaagtcgacagcttgccgtctctagctccggtgcctatataaagcagcccgctttccacatttcatattcgttttacgtttgtcaagcctcatagccggcagttcgaacgtatacgctctctgagtcagacctcgaaatcgtagctctacacaattctgtgaattttccttgtcgcgtgtgaaacacttccaataaaaactcctgcaggtgagtactttaaaaaaaaatctagtgaaataatgctgaaaagaaatttgtgtgggcaaaattcaatgggcaaaaacgcgatgcggctttttctcaaaatggcggccggcctgcgttttttcctcaaaagtgatgacgtcatgcctgttttttttttttgttcgcaatgaggaatggctcttaaaatctactagataaaaaaaatattcattatttctatgctgctggaacgcttcattaatcttaaaaattctaaattcggttaccatgatacttcgacgcataactgtagattttggatagaattaaagagaaaatggcgagagagtaaaattccggcgtcggcaaagtagagcaaaaaaatcagtataccatttagctacctctctcactcgcacgcagtgccggctcaagttgggcgcggctctgcaattatcgattttcttggggtgtgtaactaatcatccgttttcccttcctcctcatccacagcgtgaaGGTACCGCGGGCCCGGGATCCACCGGTCGCCACCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAAAGCGGCCGCataggccactagtggatctcctaggtctagagtcgacctcgaacgttaacgttaacgtaacgttaaCTCGAGCTAGATCATAATCAGCCATACCACATTTGTAAAGGTTTTACCGCGAGAAAAAACCTCCCACACCTCCCCCTGAACCTGAAACATAACTTGTCTGCGTTGTTGTTGTTAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATgtcgaggtcgacctgcagcccaagctttgcgtactcgcaaattattaaaaataaaactttaaaaataatttcgtctaattaatattatgagttaattcaaaccccacggacatgctaagggttaatcaacaatcatatcgctgtctcactcagactcaatacgacactcagaatactattcctttcactcgcacttattgcaagcatacgttaagtggatgtctcttgccgacgggaccaccttatgttatttcatcatggtctggccattctcatcgtgagcttccgggtgctcgcatatctggctctaagacttcgggcccgacgcaaggagtagccgacatatatccgaaataactgcttgtttttttttttaccattattaccatcgtgtttactgtttattgccccctcaaaaagctaatgtaattatatttgtgccaataaaaacaagatatgacctatagaatacaagtatttccccttcgaacatccccacaagtagactttggatttgtcttctaaccaaaagacttacacacctgcataccttacatcaaaaactcgtttatcgctacataaaacaccgggatatattttttatatacatacttttcaaatcgcgcgccctcttcataattcacctccaccacaccacgtttcgtagttgctctttcgctgtctcccacccgctctccgcaacacattcaccttttgttcgacgaccttggagcgactgtcgttagttccgcgcgattcggtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagcattgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccttcttcttgaactcgggctcggtgccagtatacctcaaatggttgtcgtacctctcatggttccgttacgccaacgagggtctgctgattaaccaatgggcggacgtggagccgggcgaaattagctgcacatcgtcgaacaccacgtgccccagttcgggcaaggtcatcctggagacgcttaacttctccgccgccgatctgccgctggactacgtgggtctggcccatgatgaaataacataaggtggtcccgtcgatagccgaagcttaccgaagtatacacttaaattcagtgcacgtttgcttgttgagaggaaaggttgtgtgcggacgaatttttttttgaaaacattaacccttacgtggaataaaaaaaaatgaaatattgcaaattttgctgcaaagctgtgactggagtaaaattaattcacgtgccgaagtgtgctattaagagaaaattgtgggagcagagccttgggtgcagccttggtgaaaactcccaaatttgtgatacccactttaaagattcgcagtggaaggctgcacctgcaaaaggtcagacatttaaaaggaggcgactcaacgcagatgccgtacctagtaaagtgatagagcctgaaccagaaaagataaaagaaggctataccagtgggagtacacaaacagagtaagtttgaatagtaaaaaaaatcatttatgtaaacaataacgtgactgtgcgttaggtcctgttcattgtttaatgaaaataagagcttgagggaaaaaattcgtactttggagtacgaaatgcgtcgtttagagcagcagccgaattaattctagttccagtgaaatccaagcattttctaaattaaatgtattcttattattatagttgttatttttgatatatataaacaacactattatgcccaccatttttttgagatgcatctacacaaggaacaaacactggatgtcactttcagttcaaattgtaacgctaatcactccgaacaggtcacaaaaaattaccttaaaaagtcataatattaaattagaataaatatagctgtgagggaaatatatacaaatatattggagcaaataaattgtacatacaaatatttattactaatttctattgagacgaaatgaaccactcggaaccatttgagcgaaccgaatcgcgcggaactaacgacagtcgctccaaggtcgtcgaacaaaaggtgaatgtgttgcggagagcgggtgggagacagcgaaagagcaactacgaaacgtggtgtggtggaggtgaattatgaagagggcgcgcgatttgaaaagtatgtatataaaaaatatatcccggtgttttatgtagcgataaacgagtttttgatgtaaggtatgcaggtgtgtaagtcttttggttagaagacaaatccaaagtctacttgtggggatgttcgaaggggaaatacttgtattctataggtcatatcttgtttttattggcacaaatataattacattagctttttgagggggcaataaacagtaaacacgatggtaataatggtaaaaaaaaaaacaagcagttatttcggatatatgtcggctactccttgcgtcgggcccgaagtcttagagccagatatgcgagcacccggaagctcacgatgagaatggccagacccacgtagtccagcggcagatcggcggcggagaagttaagcgtctccaggatgaccttgcccgaactggggcacgtggtgttcgacgatgtgcagctaatttcgcccggctccacgtccgcccattggttaatcagcagaccctcgttggcgtaacggaaccatgagaggtacgacaaccatttgaggtatactggcaccgagcccgagttcaagaagaagccgccaaagagcaggaatggtatgataaccggcggacccacagacagcgccatcgaggtcgaggagctggcgcaggatattagatatccgaaggacgttgacacattggccaccagagtgaccagcgccaggcagttgaagaagtgcagcactccggcccgcagtccgatcatcggataggcaatcgccgtgaagaccagtggcactgtgagaaaaagcggtaattcggcaatcgttttgcccagaaagtatgtgtcacagcgataaagtcgacttcgggcctccctcataaaaactggcagctctgaggtgaacacctaaatcgaatcgattcattagaaagttagtaaattattaatatgcaaatgtattctaaacaagacttacatttatcgtggcaaagacgttttgaaaggtcatgttggtcaggaagaggaagatggctccgttgatattcatcacgcccacttgcgtgagttgttggcccaaaaagatgaggccaatcaagatggcaaccatctgcaaattaaaatgttactcgcatctcattaatattcatatcttcaacatgttcgcgagttaaatgaaatttatttattttctgcaaaactataaactatacatctcattgaaaaaaactaagaagggtgtggaatcaggcaattctaactaaaatctagcgaatttgtttccaagaattgtaagcgttatatcatttgtttccactggaaccactcaccgttgtctgaataagtcgcacttttacgaggagtggttccttgagcaccgacagccaggatcgccacaggaccgcccggaactgcatgaaccaggtggccttgtaggtgtacccattctccggctgctccagtggcttctccaaatttttggtggccaacaactgctccatatcccgggctactttgctaatagcaaaattgtcgccatatcttggcgatccgatcacgggactcgatctcccgtccgggcacaacggccaacacctgtacgtaaaagtccgccggattgtagttggtaggacactgggcacccacgctggataggagttgagatgtaatgtaatgctagatacccttaataaacacatcgaactcactaggaaaagaagtcgacggcttcgctgggagtgcccaagaaagctaccctgccctcggccatcagaaggatcttgtcaaagagctcaaacagctcggaagacggctgatgaatggtcaggatgacggtcttgcccttctgcgacagcttcttcagcacctggacgacgctgtgggcggtaaatgagtccagtccggaggtgggctcatcgcagatcagaagcggcggatcggttagtgcctcggaggcgaatgccagacgcttcctttctccgccggacagacctttcaccctgccgggcacaccgatgatcgtgtgctgacatttgctgagcgaaagctcctggatcacctgatccacgcgggccactcgctgccgataggtcagatgtcgtggcatccgcaccatggcctggaaaatcaggtgttccctggccgttagggagccgataaagaggtcatcctgctggacataggcgcacctggcctgcatctccttggcgtccacaggttggccattgagcagtcgcatcccggatggcgatacttggatgccctgcggcgatcgaaaggcaagggcattcagcagggtcgtctttccggcaccggaactgcccatcacggccaaaagttcgcccggataggccacgccgcaaactgagtttcaaattggtaattggaccctttattaagatttcacacagatcagccgactgcgaatagaaactcaccgttcttgagcaaatgtttcctgggcgccggtatgtgtcgctcgttgcagaatagtccgcgtgtccggttgaccagctgccgccatccggagcccggctgattgaccgccccaaagatgtccatattgtgccaggcataggtgaggttctcggctagttggccgctccctgaaccggagtcctccggcggactgggtggcaggagcgtgccgtagtttttggcctgcccgaagccctggttaatgcagctctgcgaagcgtccgctgtcaccctgcaatgataggggatctcaaatatcaactacaagcgttatgctcatctaaccccgaacaaaacgaagtatcctacgaagtaggtttatacttttatttattttttgtgcatctaggatcagcttaaaatatctggttgttatattttttgtaaaaaagaatgtagtcgaaaatgaatgcctttagatgtcttgatcatgatatgatcttaaaaattgtcttatatagcgagcacagctaccagaataatctgtttcgtgtcactatttgtttgtgcgattgcggtttgggatttttgtgggtcgcagttctcacgccgcagacaatttgatgttgcaatcgcagttcctatagatcaagtgaacttaagatgtatgcacatgtactactcacattgttcagatgctcggcagatgggtgtttgctgcctccgcgaattaatagctcctgatcctcttggcccattgccgggatttttcacactttcccctgcttacccacccaaaaccaatcaccaccccaatcactcaaaaaacaaacaaaaataagaagcgagaggagttttggcacagcactttgtgtttaattgatggcgtaaaccgcttggagcttcgtcacgaaaccgctgacaaagtgcaactgaaggcggacattgacgctaggtaacgctacaaacggtggcgaaagagatagcggacgcagcggcgaaagagacggcgatatttctgtggacagagaaggaggcaaacagcgctgactttgagtggaatgtcattttgagtgagaggtaatcgaaagaacctggtacttcaaatacccttggatcgaagtaaatttaaaactgatcagataagttcaatgatatccagtgcagtaaaaaataaaaaaaaaatatgtttttttaaatctacattctccaaaaaagggttttattaacttacatacatacta
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